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Literature summary extracted from

  • Grimshaw, J.P.; Stirnimann, C.U.; Brozzo, M.S.; Malojcic, G.; Gruetter, M.G.; Capitani, G.; Glockshuber, R.
    DsbL and DsbI form a specific dithiol oxidase system for periplasmic arylsulfate sulfotransferase in uropathogenic Escherichia coli (2008), J. Mol. Biol., 380, 667-680.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.22 thioredoxin-like protein DsbL besides the role of DsbL in the formation of the structural disulfide bond of ASST, it might improve the folding efficiency of ASST in vivo by preventing unspecific aggregation of ASST Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.22 expression in Escherichia coli K12 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.22 periplasm
-
 Escherichia coli
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.2.22 128000
-
 size-exclusion chromatography, homodimer Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.22 an aryl sulfate + a phenol Escherichia coli periplasmic ASST is an in vivo substrate of DsbL, the most reactive oxidizing thioredoxin-like protein known to date, ASST activity is detected only when both functional proteins DsbL and DsbI are coexpressed a phenol + an aryl sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.22 Escherichia coli Q8FDI4 virulent uropathogenic Escherichia coli strain CFT073, isolated from the blood of a patient with acute pyelonephritis, is cytotoxic for human renal cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.22 chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.22 an aryl sulfate + a phenol
-
 Escherichia coli a phenol + an aryl sulfate
-
 ?
2.8.2.22 an aryl sulfate + a phenol periplasmic ASST is an in vivo substrate of DsbL, the most reactive oxidizing thioredoxin-like protein known to date, ASST activity is detected only when both functional proteins DsbL and DsbI are coexpressed Escherichia coli a phenol + an aryl sulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.2.22 homodimer 1 * 128000Da, size-exclusion chromatography Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.8.2.22 arylsulfate sulfotransferase
-
 Escherichia coli
2.8.2.22 ASST
-
 Escherichia coli