EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.2.27 | gene TtUDGB, expression of wild-type and selenomethionine-labeled enzymes in Escherichia coli strain BL21(DE3) | Thermus thermophilus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.2.27 | recombinant family 5 UDGB, as free protein or selenomethionine-labeled protein, or in complex with rAP-G DNA and rAP-A DNA, hanging drop vapor diffusion method, 0.001 ml drops of 11 mg/ml TtUDGB are mixed with 0.001 ml of 9-13% v/v PEG 3350, 0.2 M ammonium acetate, 5% v/v glycerol and 0.1 M MES, pH 6.5, and equilibrated against 0.5 ml of the reservoir solution at 20°C. X-ray diffraction structure determination and analysis at 1.45-2.1 A resolution, molecular replacement | Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.2.27 | D75A | the D75A mutant shows low enzymatic activity for the removal of uracil from U-G or thymine from T-G. However, the mutant can distinguish between the C5-hydrogen and the C5-methyl group | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.27 | additional information | Thermus thermophilus | UDG removes uracil generated by the deamination of cytosine or misincorporation of deoxyuridine monophosphate. The fifth UDG family lacks a polar residue in the active-site motif, which mediates the hydrolysis of the glycosidic bond by activation of a water molecule in UDG families 1 to 4 | ? | - |
? | |
3.2.2.27 | additional information | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | UDG removes uracil generated by the deamination of cytosine or misincorporation of deoxyuridine monophosphate. The fifth UDG family lacks a polar residue in the active-site motif, which mediates the hydrolysis of the glycosidic bond by activation of a water molecule in UDG families 1 to 4 | ? | - |
? | |
3.2.2.27 | uracil-mismatched double-stranded DNA + H2O | Thermus thermophilus | - |
uracil + double-stranded DNA with abasic site | - |
? | |
3.2.2.27 | uracil-mismatched double-stranded DNA + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
uracil + double-stranded DNA with abasic site | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.27 | Thermus thermophilus | Q5SJG5 | family 5 UDGB, gene TtUDGB | - |
3.2.2.27 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SJG5 | family 5 UDGB, gene TtUDGB | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.2.27 | recombinant wild-type and selenomethionine-labeled enzymes from Escherichia coli strain BL21(DE3) by hydrophobic interaction and anion exchange chromatography, followed by hydroxyapatite adsorption chromatography and gel filtration | Thermus thermophilus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.2.2.27 | Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil | a conserved asparagine residue acts as a ligand to the catalytic water molecule, and another water molecule acts as a barrier during substrate recognition, slide-in mechanism for initial damage recognition, catalytic reaction mechanism, overview | Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.27 | additional information | UDG removes uracil generated by the deamination of cytosine or misincorporation of deoxyuridine monophosphate. The fifth UDG family lacks a polar residue in the active-site motif, which mediates the hydrolysis of the glycosidic bond by activation of a water molecule in UDG families 1 to 4 | Thermus thermophilus | ? | - |
? | |
3.2.2.27 | additional information | family 5 UDGB in complex with rAP-G DNA and rAP-A DNA, substrate specificity and binding structure analysis, modelling, overview | Thermus thermophilus | ? | - |
? | |
3.2.2.27 | additional information | UDG removes uracil generated by the deamination of cytosine or misincorporation of deoxyuridine monophosphate. The fifth UDG family lacks a polar residue in the active-site motif, which mediates the hydrolysis of the glycosidic bond by activation of a water molecule in UDG families 1 to 4 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
3.2.2.27 | additional information | family 5 UDGB in complex with rAP-G DNA and rAP-A DNA, substrate specificity and binding structure analysis, modelling, overview | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
3.2.2.27 | uracil-mismatched double-stranded DNA + H2O | - |
Thermus thermophilus | uracil + double-stranded DNA with abasic site | - |
? | |
3.2.2.27 | uracil-mismatched double-stranded DNA + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | uracil + double-stranded DNA with abasic site | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.2.27 | More | family 5 UDGB secondary structure elements, overview | Thermus thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.2.27 | More | the enzyme is an UDG family 5 enzyme belonging to the UDG superfamily | Thermus thermophilus |
3.2.2.27 | UDG | - |
Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.2.27 | 37 | - |
assay at | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.2.27 | 7.5 | - |
assay at | Thermus thermophilus |