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Literature summary extracted from

  • Balbo, P.B.; Toth, J.; Bohm, A.
    X-ray crystallographic and steady state fluorescence characterization of the protein dynamics of yeast polyadenylate polymerase (2007), J. Mol. Biol., 366, 1401-1415.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.19 hanging drop vapour diffusion method, using 20% (w/v) PEG 8000, 100 mM magnesium acetate, 100 mM imidazole (pH 6.2), 3% ethylene glycol Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.19 0.0197
-
 2-aminopurine riboside triphosphate
-
 Saccharomyces cerevisiae
2.7.7.19 0.0359
-
 ATP
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.19 Mg2+ required for activity Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.19 2-aminopurine riboside triphosphate + RNA Saccharomyces cerevisiae
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.19 Saccharomyces cerevisiae P29468
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.19 Ni-affinity column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.19 2-aminopurine riboside triphosphate + RNA
-
 Saccharomyces cerevisiae ?
-
 ?
2.7.7.19 ATP + RNA PAP catalyzes the synthesis of poly(A) tails on the 3'-end of pre-mRNA Saccharomyces cerevisiae diphosphate + RNA(A)n
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.19 PAP
-
 Saccharomyces cerevisiae
2.7.7.19 polyadenylate polymerase
-
 Saccharomyces cerevisiae