Any feedback?
Literature summary extracted from
- Effects of calcium ion concentration on starch hydrolysis of barley alpha-amylase isozymes (2008), J. Microbiol. Biotechnol., 18, 730-734.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | cloned and overexpressed in Pichia pastoris, recombinant isoenzymes with a six-histidine fusion tag | Hordeum vulgare |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | Ca2+ | AMY1 shows the highest activity at 5 mM calcium concentration and maintains its activity at a broad range from 0.1 to 10 mM of calcium ion. AMY2 shows the highest acitivity at 15-20 mM of calcium, compared with the lowest 20% of activity at 0.1 mM of calcium concentration, 37°C, substrate insoluble blue starch. There is no significant difference in hydrolyzing activity on the soluble starch substrate between AMY1 and AMY2, with the increase of calcium concentration. The increase in calcium up to 50 mM causes the decrease by 0-30% in the activity of both AMYs | Hordeum vulgare |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 45000 | - |
AMY1 or AMY2, SDS-PAGE | Hordeum vulgare |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Hordeum vulgare | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | recombinant enzyme | Hordeum vulgare |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
AMY1 with various substrates: 25.74 U/mg with insoluble blue starch, 132.64 U/mg with corn starch, 189.31 U/mg with potato starch, 153.28 U/mg with soluble starch (Showa Chemical Co.), and 296.6 U/mg with soluble starch (Sigma-Aldrich). AMY2 with various substrates: 17.64 U/mg with insoluble blue starch, 131.71 U/mg with corn starch, 222.34 U/mg with potato starch, 272.68 U/mg with soluble starch (Showa Chemical Co.), and 210.24 U/mg with soluble starch (Sigma-Aldrich) | Hordeum vulgare |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | maltoheptaose + H2O | - |
Hordeum vulgare | maltotriose + maltose + D-glucose | identified by thin-layer-chromatography | ? | |
| 3.2.1.1 | maltohexaose + H2O | - |
Hordeum vulgare | maltotetraose + maltotriose + maltose + D-glucose | identified by thin-layer-chromatography | ? | |
| 3.2.1.1 | maltopentaose + H2O | - |
Hordeum vulgare | maltotetraose + maltotriose + maltose + D-glucose | identified by thin-layer-chromatography | ? | |
| 3.2.1.1 | maltotetraose + H2O | - |
Hordeum vulgare | maltotriose + maltose | identified by thin-layer-chromatography | ? | |
| 3.2.1.1 | maltotriose + H2O | - |
Hordeum vulgare | maltose + D-glucose | identified by thin-layer-chromatography | ? | |
| 3.2.1.1 | starch + H2O | - |
Hordeum vulgare | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | ? | x * 45000, AMY1 or AMY2, SDS-PAGE | Hordeum vulgare |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | AMY1 | isoenzyme | Hordeum vulgare |
| 3.2.1.1 | AMY2 | isoenzyme | Hordeum vulgare |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 3.5 | - |
AMY1 is more stable at low pH than AMY2. The activity of AMY2 rapidly decreases to 51% after 3 min incubation at pH 3.5 | Hordeum vulgare |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
