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Literature summary extracted from
- Bifunctional recombinant fusion enzyme between maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase of thermophilic microorganism Metallosphaera hakonensis (2008), J. Microbiol. Biotechnol., 18, 1544-1549.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.141 | Escherichia coli BL21(DE3)pLysS, LB medium, vector pRSET-B | Metallosphaera hakonensis |
| 5.4.99.15 | expressed in Escherichia coli BL21(DE3)pLysS cells as fusion enzyme with maltooligosyltrehalose trehalohydrolase | Metallosphaera hakonensis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.141 | additional information | bifunctional fusion enzyme (MhMTSH) of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH) | Metallosphaera hakonensis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.141 | 150000 | - |
SDS-PAGE, in accordance with the calculated value from the amino acid sequence, fusion enzyme including the hexahistidine domain from the vector | Metallosphaera hakonensis |
| 5.4.99.15 | 150000 | - |
MTSH fusion enzyme | Metallosphaera hakonensis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.141 | Metallosphaera hakonensis | - |
grown on JCM165 medium at 70°C | - |
| 3.2.1.141 | Metallosphaera hakonensis JMC8857 | - |
grown on JCM165 medium at 70°C | - |
| 5.4.99.15 | Metallosphaera hakonensis | - |
strain JCM8857 | - |
| 5.4.99.15 | Metallosphaera hakonensis JCM 8857 | - |
strain JCM8857 | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.141 | additional information | - |
5 mM maltooligosaccharides, 100mM sodium phosphate buffer, pH 5, 70°C, 30 min, with substrate soluble starch 24 h | Metallosphaera hakonensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.141 | 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O | hydrolysis to disaccharides after 24 h | Metallosphaera hakonensis | (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose | - |
? |  |
| 3.2.1.141 | 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O | hydrolysis to disaccharides after 24 h | Metallosphaera hakonensis JCM 8857 | (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose | - |
? | |
| 3.2.1.141 | 4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O | hydrolysis to disaccharides after 24 h | Metallosphaera hakonensis JMC8857 | (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose | - |
? | |
| 3.2.1.141 | maltotriosyltrehalose + H2O | sequential hydrolysis by the bifunctional fusion enzyme of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH), synthase converts alpha-1,4-glycosidic linkage into alpha-1,1 linkage to produce maltotriosyltrehalose, which is the substrate for the MTH to produce trehalose | Metallosphaera hakonensis | maltotriose + alpha,alpha'-trehalose | - |
? | |
| 3.2.1.141 | maltotriosyltrehalose + H2O | sequential hydrolysis by the bifunctional fusion enzyme of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH), synthase converts alpha-1,4-glycosidic linkage into alpha-1,1 linkage to produce maltotriosyltrehalose, which is the substrate for the MTH to produce trehalose | Metallosphaera hakonensis JCM 8857 | maltotriose + alpha,alpha'-trehalose | - |
? | |
| 3.2.1.141 | maltotriosyltrehalose + H2O | sequential hydrolysis by the bifunctional fusion enzyme of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH), synthase converts alpha-1,4-glycosidic linkage into alpha-1,1 linkage to produce maltotriosyltrehalose, which is the substrate for the MTH to produce trehalose | Metallosphaera hakonensis JMC8857 | maltotriose + alpha,alpha'-trehalose | - |
? | |
| 5.4.99.15 | maltohexaose | - |
Metallosphaera hakonensis | alpha-maltotetraosyl trehalose | - |
? | |
| 5.4.99.15 | maltohexaose | - |
Metallosphaera hakonensis JCM 8857 | alpha-maltotetraosyl trehalose | - |
? | |
| 5.4.99.15 | maltohexaose | - |
Metallosphaera hakonensis JMC8857 | alpha-maltotetraosyl trehalose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.141 | ? | x * 150000, fusion enzyme of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH), SDS-PAGE | Metallosphaera hakonensis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.141 | maltooligosyltrehalose trehalohydrolase | - |
Metallosphaera hakonensis |
| 3.2.1.141 | MhMTH | - |
Metallosphaera hakonensis |
| 3.2.1.141 | MTH | - |
Metallosphaera hakonensis |
| 5.4.99.15 | maltooligosyltrehalose synthase | - |
Metallosphaera hakonensis |
| 5.4.99.15 | MTS | - |
Metallosphaera hakonensis |
| 5.4.99.15 | MTSH | fusion enzyme consisting of maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase | Metallosphaera hakonensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.141 | 70 | - |
- |
Metallosphaera hakonensis |
| 5.4.99.15 | 70 | - |
MTSH fusion enzyme | Metallosphaera hakonensis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.141 | 40 | 90 | fusion enzyme with 5 mM maltopentaose, 30 min: 100% activity at 70, about 90% at 60, about 80% at 50 and 80, below 40% at 40 and 90°C | Metallosphaera hakonensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.141 | 40 | 90 | 80% activity retained after 48 h pre-incubation at 70°C, activities after pre-incubation at set temperatures for 30 min: 90-100% at 40-70°C, less than 80% at 80°C, quickly abolished beyond 80°C | Metallosphaera hakonensis |
| 5.4.99.15 | 70 | 80 | the MTSH fusion enzyme exhibits a high degree of thermostability, retaining 80% of the activity when pre-incubated at 70°C for 48 h, the stability is gradually abolished by incubating the fusion enzyme at above 80°C for 30 min | Metallosphaera hakonensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.141 | 5 | 6 | - |
Metallosphaera hakonensis |
| 5.4.99.15 | 5 | 6 | MTSH fusion enzyme | Metallosphaera hakonensis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.141 | 4 | 9 | pH 4: about 70% activity, pH 5-6: about 100% activity, pH 7: about 40% activity, pH 8-9: between 20-30%, pH 4-5: 0.1 M McIlvaine buffer, pH 5-8: 0.1 M sodium phosphate buffer, pH 8-9: 0.1 M sodium bicarbonate buffer, 70°C, 30 min | Metallosphaera hakonensis |
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