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Literature summary extracted from

  • Wiater, A.; Szczodrak, J.; Pleszczynskaa, M.
    Mutanase Induction in Trichoderma harzianum by Cell Wall of Laetiporus sulphureus and its Application for Mutan Removal from Oral Biofilms (2008), J. Microbiol. Biotechnol., 18, 1335-1341.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.84 alpha-1,3-glucan streptococcal mutan, induction Trichoderma harzianum
3.2.1.84 cell wall material from fruiting bodies of Laetiporus sulphureus induction Trichoderma harzianum

Application

EC Number Application Comment Organism
3.2.1.84 medicine removal of oral biofilms; possible active ingredients in chewing gum, mouthwash, dental gel, toothpaste Trichoderma harzianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.84 extracellular
-
Trichoderma harzianum
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.84 Trichoderma harzianum
-
rich in alpha-(1-3)-glucans
-
3.2.1.84 Trichoderma harzianum F-340
-
rich in alpha-(1-3)-glucans
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.84 yield of 56.3% of alkali-soluble and water-insoluble D-glucan Trichoderma harzianum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.84 additional information
-
medium supplementation with more than 0.4% cell wall preparation results in lower specific activity Trichoderma harzianum
3.2.1.84 0.178
-
after incubation for 1 h at 45°C, medium supplementation with 2.0% cell wall preparation of Laetiporus sulphureus Trichoderma harzianum
3.2.1.84 1.72
-
after incubation for 1 h at 45°C, medium supplementation with 0.4% cell wall preparation of Laetiporus sulphureus Trichoderma harzianum

Storage Stability

EC Number Storage Stability Organism
3.2.1.84 45°C, 12 h Trichoderma harzianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.84 alpha-1,3-glucan + H2O
-
Trichoderma harzianum alpha-D-glucose
-
?
3.2.1.84 alpha-1,3-glucan + H2O
-
Trichoderma harzianum F-340 alpha-D-glucose
-
?
3.2.1.84 sucrose + H2O
-
Trichoderma harzianum D-glucose + D-fructose
-
?
3.2.1.84 sucrose + H2O
-
Trichoderma harzianum F-340 D-glucose + D-fructose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.84 mutanase
-
Trichoderma harzianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.84 45
-
-
Trichoderma harzianum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.84 45
-
stable for 12 h Trichoderma harzianum