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Literature summary extracted from
- Cloning and characterization of thermostable esterase from Archaeoglobus fulgidus (2008), J. Microbiol., 46, 100-107.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.1 | expressed in Escherichia coli | Archaeoglobus fulgidus |
| 3.1.1.2 | expressed in Escherichia coli | Archaeoglobus fulgidus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.1 | 1.6 | - |
ketoprofen ethyl ester | - |
Archaeoglobus fulgidus |  |
| 3.1.1.2 | 1.6 | - |
ketoprofen ethyl ester | at pH 8.0 | Archaeoglobus fulgidus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 27500 | - |
gel filtration | Archaeoglobus fulgidus |
| 3.1.1.2 | 27500 | - |
estimated from amino acid sequence | Archaeoglobus fulgidus |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.1.1.1 | Triton X-100 | 960% increase of activity in the presence of Triton X-100 | Archaeoglobus fulgidus |
| 3.1.1.1 | Triton X-15 | 110% increase of activity in the presence of Triton X-15 | Archaeoglobus fulgidus |
| 3.1.1.1 | Triton X-165 | 1649% increase of activity in the presence of Triton X-15 | Archaeoglobus fulgidus |
| 3.1.1.1 | Triton X-305 | 1528% increase of activity in the presence of Triton X-15 | Archaeoglobus fulgidus |
| 3.1.1.1 | Triton X-45 | 562% increase of activity in the presence of Triton X-15 | Archaeoglobus fulgidus |
| 3.1.1.1 | Tween | more than 1200% increase of activity in the presence of Tween 20, 40, 60 or 80 | Archaeoglobus fulgidus |
| 3.1.1.2 | additional information | organic solvents addition (acetone, acetonitrile, iso-propanol, ethanol, methanol, dimethyl sulfoxide, and dimethyl formamide) is not suitable for improvement of enzyme reaction through increase of substrate solubility | Archaeoglobus fulgidus |
| 3.1.1.2 | Triton X-100 | 1060% relative activity in the presence of 1% (v/v) Triton X-100 | Archaeoglobus fulgidus |
| 3.1.1.2 | Triton X-15 | 210% relative activity in the presence of 1% (v/v) Triton X-15 | Archaeoglobus fulgidus |
| 3.1.1.2 | Triton X-165 | 1749% relative activity in the presence of 1% (v/v) Triton X-165 | Archaeoglobus fulgidus |
| 3.1.1.2 | Triton X-305 | 1628% relative activity in the presence of 1% (v/v) Triton X-305 | Archaeoglobus fulgidus |
| 3.1.1.2 | Triton X-45 | 662% relative activity in the presence of 1% (v/v) Triton X-45 | Archaeoglobus fulgidus |
| 3.1.1.2 | Tween 20 | 1396% relative activity in the presence of 1% (v/v) Tween 20 | Archaeoglobus fulgidus |
| 3.1.1.2 | Tween 40 | 1306% relative activity in the presence of 1% (v/v) Tween 40 | Archaeoglobus fulgidus |
| 3.1.1.2 | Tween 60 | 1396% relative activity in the presence of 1% (v/v) Tween 60 | Archaeoglobus fulgidus |
| 3.1.1.2 | Tween 80 | 1339% relative activity in the presence of 1% (v/v) Tween 80 | Archaeoglobus fulgidus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.1 | Archaeoglobus fulgidus | - |
strain DSM 4304 | - |
| 3.1.1.2 | Archaeoglobus fulgidus | - |
strain DSM 4304 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.1 | Ni-NTA column chromatography | Archaeoglobus fulgidus |
| 3.1.1.2 | Ni+2-NTA resin chromatography | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.1 | 4-nitrophenyl acetate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + acetate | - |
? | |
| 3.1.1.1 | 4-nitrophenyl butyrate + H2O | highest activity | Archaeoglobus fulgidus | 4-nitrophenol + butyrate | - |
? | |
| 3.1.1.1 | ketoprofen ethyl ester + H2O | - |
Archaeoglobus fulgidus | ketoprofen + ethanol | - |
? | |
| 3.1.1.2 | 4-nitrophenyl butyrate + H2O | highest specific activity | Archaeoglobus fulgidus | 4-nitrophenol + butyrate | - |
? | |
| 3.1.1.2 | 4-nitrophenyl caprylate + H2O | 10% activity compared to 4-nitrophenyl butyrate | Archaeoglobus fulgidus | 4-nitrophenol + caprylate | - |
? | |
| 3.1.1.2 | 4-nitrophenyl hexanoate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + hexanoate | - |
? | |
| 3.1.1.2 | ketoprofen ethyl ester + H2O | - |
Archaeoglobus fulgidus | ? | - |
? | |
| 3.1.1.2 | additional information | no activity with 4-nitrophenyl laurate and 4-nitrophenyl palmitate | Archaeoglobus fulgidus | ? | - |
? | |
| 3.1.1.2 | additional information | the enzyme is a carboxylesterase and has highest activity with 4-nitrophenyl butyrate, activity decreases with acylglycerols containing longer carbon chains | Archaeoglobus fulgidus | ? | - |
? | |
| 3.1.1.2 | phenyl acetate + H2O | - |
Archaeoglobus fulgidus | phenol + acetate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.1 | monomer | 1 * 27500, gel filtration | Archaeoglobus fulgidus |
| 3.1.1.2 | monomer | 1 * 27500, estimated from amino acid sequence | Archaeoglobus fulgidus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.1 | Est-AF | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 70 | - |
using ketoprofen ethyl ester as substrate | Archaeoglobus fulgidus |
| 3.1.1.1 | 80 | - |
using 4-nitrophenyl butyrate as substrate | Archaeoglobus fulgidus |
| 3.1.1.2 | 70 | - |
using ketoprofen ethyl ester as substrate | Archaeoglobus fulgidus |
| 3.1.1.2 | 80 | - |
using 4-nitrophenyl butyrate as substrate | Archaeoglobus fulgidus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.2 | 40 | 90 | 10% of activity is retained after 3 h at 90°C using ketoprofen ethyl ester as substrate, the enzyme shows about 50% activity at 40°C, 68% relative activity at 50°C, about 90% activity at 60°C, 100% activity at 70°C, about 90% activity at 80°C, and 58% activity at 90°C | Archaeoglobus fulgidus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 65 | 90 | more than 90% activity remains at 65°C or 70°C, 10% of activity remains after 3 h incubation at 90°C using ketoprofen ethyl ester as substrate | Archaeoglobus fulgidus |
| 3.1.1.2 | 65 | - |
no loss of activity after 180 min at 65°C | Archaeoglobus fulgidus |
| 3.1.1.2 | 70 | - |
no loss of activity after 180 min at 70°C | Archaeoglobus fulgidus |
| 3.1.1.2 | 75 | - |
about 20% loss of activity after 180 min at 75°C | Archaeoglobus fulgidus |
| 3.1.1.2 | 80 | - |
about 30% loss of activity after 180 min at 80°C | Archaeoglobus fulgidus |
| 3.1.1.2 | 85 | - |
about 40% loss of activity after 180 min at 85°C | Archaeoglobus fulgidus |
| 3.1.1.2 | 90 | - |
about 50% loss of activity after 80 min at 90°C | Archaeoglobus fulgidus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 7 | 8 | - |
Archaeoglobus fulgidus |
| 3.1.1.2 | 7 | 8 | - |
Archaeoglobus fulgidus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.2 | 3 | 12 | enzyme activity is decreased higher than pH 8.0, no activity at pH 3.0 and 12.0, about 10% relative activity is retained at pH 4 and 11 | Archaeoglobus fulgidus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.1 | 3 | 12 | enzyme activity decreases above pH 8.0, no enzyme activity is observed at pH 3.0 and 12.0 | Archaeoglobus fulgidus |
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