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Literature summary extracted from

  • Chiriac, M.; Lupan, I.; Bucurenci, N.; Popescu, O.; Palibroda, N.
    Stereoselective synthesis of L-[15N] amino acids with glucose dehydrogenase and galactose mutarotase as NADH regenerating system (2008), J. Labelled Compd. Radiopharm., 51, 171-174.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.4.1.9 biotechnology an efficient stereospecific enzymatic synthesis of L-valine, L-leucine, L-norvaline, L-norleucine and L-isoleucine from the corresponding alpha-keto acids by coupling the reactions catalysed by leucine dehydrogenase and glucose dehydrogenase/galactose mutarotase. Giving high yields of L-amino acids, the procedure is economical and easy to perform and to monitor at a synthetically useful scale (1-10 g) Geobacillus stearothermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.9 overexpressed in Escherichia coli Geobacillus stearothermophilus
5.1.3.3 efficient stereospecific enzymatic synthesis of L-[15N]-valine, L-[15N]-leucine, L-[15N]-norvaline, L-[15N]-norleucine and L-[15N]-isoleucine from the corresponding alpha-keto acids by coupling the reactions catalysed by leucine dehydrogenase and glucose dehydrogenase/galactose mutarotase. Giving high yields of L-amino acids, the procedure is economical and easy to perform and to monitor at a synthetically useful scale of 1-10 g. Substrates alpha-ketoisovalerate, alpha-ketoisocaproate, alpha-ketovalerate, alpha-ketocaproateand alpha-keto-beta-methylvalerate are converted to products L-valine, L-leucine, L-norvaline, L-isoleucine with yields of 90%, 92.5%, 92%, 80%, and 95%, respectively Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.9 Geobacillus stearothermophilus P13154
-
-
5.1.3.3 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.9 alpha-keto beta-methylvalerate + NH3 + NADH + H+ yield: 95% Geobacillus stearothermophilus L-isoleucine + H2O + NAD+
-
 ?
1.4.1.9 alpha-ketocaproate + NH3 + NADH + H+ yield: 80% Geobacillus stearothermophilus L-norleucine + H2O + NAD+
-
 ?
1.4.1.9 alpha-ketoisocaproate + NH3 + NADH + H+ yield: 92.5% Geobacillus stearothermophilus L-leucine + H2O + NAD+
-
 ?
1.4.1.9 alpha-ketoisovalerate + NH3 + NADH + H+ yield: 90% Geobacillus stearothermophilus L-valine + H2O + NAD+
-
 ?
1.4.1.9 alpha-ketovalerate + NH3 + NADH + H+ yield: 92% Geobacillus stearothermophilus L-norvaline + H2O + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.1.9 LeuDH
-
 Geobacillus stearothermophilus
5.1.3.3 GalM
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.9 30
-
 assay at Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.9 8
-
 assay at Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.9 NADH
-
 Geobacillus stearothermophilus