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Literature summary extracted from
- Lipid mediators and vector infection: Trypanosoma rangeli inhibits Rhodnius prolixus hemocyte phagocytosis by modulation of phospholipase A2 and PAF-acetylhydrolase activities (2008), J. Insect Physiol., 54, 1528-1537.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.4 | dexamethasone | inhibitor of PLA2, phagocytosis of hemocyte monolayers is significantly reduced in cells provided by insects treated with dexamethasone (0.125 mM), when compared to untreated controls or hemocytes taken from insects treated with dexamethasone but that receive inoculation of arachidonic acid or platelet activating factor. Inhibits total PLA2 activity in lysates from hemocytes by 42%. Cell-free hemolymph PLA2 activity is not affected by dexamethasone treatment (slight but not significant inhibition) | Rhodnius prolixus |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.4 | Ca2+ | soluble PLA2 shows Ca+2-dependent and independent fractions, corresponding to 3% or 68% of total activity, respectively | Rhodnius prolixus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.4 | Rhodnius prolixus | - |
- |
- |
| 3.1.1.47 | Rhodnius prolixus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.4 | hemocyte | - |
Rhodnius prolixus | - |
| 3.1.1.4 | hemolymph | - |
Rhodnius prolixus | - |
| 3.1.1.4 | larva | - |
Rhodnius prolixus | - |
| 3.1.1.47 | hemocyte | - |
Rhodnius prolixus | - |
| 3.1.1.47 | hemolymph | - |
Rhodnius prolixus | - |
| 3.1.1.47 | larva | - |
Rhodnius prolixus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.47 | 4.5 | - |
Ca2+-dependent PLA2, cell-free hemolymph of Rhodnius prolixus feeding with blood plus dexamethasone | Rhodnius prolixus |
| 3.1.1.47 | 6.7 | - |
Ca2+-dependent PLA2, control, cell-free hemolymph of Rhodnius prolixus after regular blood feeding | Rhodnius prolixus |
| 3.1.1.47 | 10.7 | - |
Ca2+-dependent PLA2, cell-free hemolymph of Rhodnius prolixus feeding with Trypanosoma rangeli epimastigotes | Rhodnius prolixus |
| 3.1.1.47 | 12 | - |
Ca2+-independent PLA2, cell-free hemolymph of Rhodnius prolixus feeding with blood plus dexamethasone | Rhodnius prolixus |
| 3.1.1.47 | 14 | - |
Ca2+-independent PLA2, control, cell-free hemolymph of Rhodnius prolixus after regular blood feeding | Rhodnius prolixus |
| 3.1.1.47 | 17 | - |
total PLA2, cell-free hemolymph of Rhodnius prolixus feeding with blood plus dexamethasone | Rhodnius prolixus |
| 3.1.1.47 | 21 | - |
total PLA2, control, cell-free hemolymph of Rhodnius prolixus after regular blood feeding | Rhodnius prolixus |
| 3.1.1.47 | 24 | - |
PAF-AH, control, cell-free hemolymph of Rhodnius prolixus after regular blood feeding | Rhodnius prolixus |
| 3.1.1.47 | 32 | - |
Ca2+-independent PLA2, cell-free hemolymph of Rhodnius prolixus feeding with Trypanosoma rangeli epimastigotes | Rhodnius prolixus |
| 3.1.1.47 | 34 | - |
PAF-AH, cell-free hemolymph of Rhodnius prolixus feeding with blood plus dexamethasone | Rhodnius prolixus |
| 3.1.1.47 | 43 | - |
total PLA2, cell-free hemolymph of Rhodnius prolixus feeding with Trypanosoma rangeli epimastigotes | Rhodnius prolixus |
| 3.1.1.47 | 119 | - |
Ca2+-independent PLA2, hemocyte of Rhodnius prolixus feeding with Trypanosoma rangeli epimastigotes | Rhodnius prolixus |
| 3.1.1.47 | 140 | - |
Ca2+-independent PLA2, hemocyte of Rhodnius prolixus feeding with blood plus dexamethasone | Rhodnius prolixus |
| 3.1.1.47 | 186 | - |
Ca2+-independent PLA2, control, hemocyte of Rhodnius prolixus after regular blood feeding | Rhodnius prolixus |
| 3.1.1.47 | 203 | - |
PAF-AH, cell-free hemolymph of Rhodnius prolixus feeding with Trypanosoma rangeli epimastigotes | Rhodnius prolixus |
| 3.1.1.47 | 381 | - |
Ca2+-dependent PLA2, hemocyte of Rhodnius prolixus feeding with blood plus dexamethasone | Rhodnius prolixus |
| 3.1.1.47 | 427 | - |
Ca2+-dependent PLA2, hemocyte of Rhodnius prolixus feeding with Trypanosoma rangeli epimastigotes | Rhodnius prolixus |
| 3.1.1.47 | 529 | - |
total PLA2, hemocyte of Rhodnius prolixus feeding with blood plus dexamethasone | Rhodnius prolixus |
| 3.1.1.47 | 570 | - |
total PLA2, hemocyte of Rhodnius prolixus feeding with Trypanosoma rangeli epimastigotes | Rhodnius prolixus |
| 3.1.1.47 | 702 | - |
Ca2+-dependent PLA2, control, hemocyte of Rhodnius prolixus after regular blood feeding | Rhodnius prolixus |
| 3.1.1.47 | 915 | - |
total PLA2, control, hemocyte of Rhodnius prolixus after regular blood feeding | Rhodnius prolixus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.4 | 1-palmitoyl-2-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-sn-glycero-3-phosphocholine + H2O | - |
Rhodnius prolixus | ? | - |
? | |
| 3.1.1.47 | 1-palmitoyl-2-[12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-sn-glycero-3-phosphocholine + H2O | substrate activity assay | Rhodnius prolixus | ? | - |
? | |
| 3.1.1.47 | 1-palmitoyl-2-[6-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]hexanoyl]-sn-glycero-3-phosphocholine + H2O | substrate activity assay | Rhodnius prolixus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.4 | Ca+2-independent PLA2 | - |
Rhodnius prolixus |
| 3.1.1.4 | cPLA2 | - |
Rhodnius prolixus |
| 3.1.1.4 | cytosolic PLA2 | - |
Rhodnius prolixus |
| 3.1.1.4 | iPLA2 | - |
Rhodnius prolixus |
| 3.1.1.4 | phospholipase A2 | - |
Rhodnius prolixus |
| 3.1.1.4 | PLA2 | - |
Rhodnius prolixus |
| 3.1.1.4 | secreted PLA2 | - |
Rhodnius prolixus |
| 3.1.1.4 | sPLA2 | - |
Rhodnius prolixus |
| 3.1.1.47 | PAF-acetylhydrolase | - |
Rhodnius prolixus |
| 3.1.1.47 | PAF-AH | - |
Rhodnius prolixus |
| 3.1.1.47 | phospholipase A2 | - |
Rhodnius prolixus |
| 3.1.1.47 | PLA2 | - |
Rhodnius prolixus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.47 | 37 | - |
activity assay | Rhodnius prolixus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.47 | 8 | - |
activity assay | Rhodnius prolixus |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.1.1.4 | Rhodnius prolixus | following oral infection with Trypanosoma rangeli, significant attenuation of PLA2 activities in hemocytes (cPLA2, sPLA2 and iPLA2) in cell-free hemolymph. Trypanosoma rangeli infection inhibits total PLA2 activity in lysates from hemocytes by 39% | down |
| 3.1.1.4 | Rhodnius prolixus | following oral infection with Trypanosoma rangeli, significant enhancement of sPLA2 activities in cell-free hemolymph | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.1.4 | physiological function | role of PLA2 in the regulation of arachidonic acid and insect platelet activating factor biosynthesis | Rhodnius prolixus |
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