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Literature summary extracted from

  • Cimini, D.; De Rosa, M.; Panariello, A.; Morelli, V.; Schiraldi, C.
    Production of a thermophilic maltooligosyl-trehalose synthase in Lactococcus lactis (2008), J. Ind. Microbiol. Biotechnol., 35, 1079-1083.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.15 expressed in Lactococcus lactis strain NZ9000 Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.15 87000
-
 SDS-PAGE Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.15 Saccharolobus solfataricus
-
-
-
5.4.99.15 Saccharolobus solfataricus MT4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.15 gel filtration Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.15 14.4
-
 recombinant enzyme, after 45.5fold purification, at 70°C Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.15 maltoheptaose preferred substrate Saccharolobus solfataricus alpha-maltopentaosyl trehalose
-
 ?
5.4.99.15 maltoheptaose preferred substrate Saccharolobus solfataricus MT4 alpha-maltopentaosyl trehalose
-
 ?
5.4.99.15 maltohexaose preferred substrate Saccharolobus solfataricus alpha-maltotetraosyl trehalose
-
 ?
5.4.99.15 maltohexaose preferred substrate Saccharolobus solfataricus MT4 alpha-maltotetraosyl trehalose
-
 ?
5.4.99.15 additional information starch, amylose and amylopectin are no good substrates, MTS is not active on maltotriose Saccharolobus solfataricus ?
-
 ?
5.4.99.15 additional information starch, amylose and amylopectin are no good substrates, MTS is not active on maltotriose Saccharolobus solfataricus MT4 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.99.15 maltooligosyl-trehalose synthase
-
 Saccharolobus solfataricus
5.4.99.15 MTS
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.15 70
-
 purified recombinant enzyme Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.99.15 75
-
 MTS proves stable at 75°C for more than 24 h, with a half life of about 50 h Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.15 5.5
-
 purified recombinant enzyme Saccharolobus solfataricus