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Literature summary extracted from

  • Wang, S.Y.; Zhou, J.J.; Shao, B.; Lu, Y.J.; Rao, P.F.
    A thermostable chitinase with chitin-binding activity from Phaseolus limensis (2008), J. Food Sci., 73, C452-C457.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Ca2+
-
 Phaseolus lunatus
3.2.1.14 Cu2+ at 2 mM Phaseolus lunatus
3.2.1.14 Mn2+ slightly inhibiting at 1-2 mM Phaseolus lunatus
3.2.1.14 Zn2+ slightly inhibiting at 1-2 mM Phaseolus lunatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 EDTA slightly activating Phaseolus lunatus
3.2.1.14 KCl slightly activating at 1-2 mM Phaseolus lunatus
3.2.1.14 additional information the enzyme does not require any metal ions for its activity, no effects on enzyme activity by Mg2+ Phaseolus lunatus
3.2.1.14 NaCl slightly activating at 1-2 mM Phaseolus lunatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 28600
-
 native PAGE Phaseolus lunatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Phaseolus lunatus
-
 ?
-
 ?
3.2.1.14 additional information Phaseolus lunatus the enzyme exerts a potent inhibitory action toward fungal species, including Fusarium solani, Pythium aphanidermatum, and Sclerotium rolfsii ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Phaseolus lunatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native enzyme 108fold from dry seeds by ammonium sulfate fractionation, affinity and cation exchange chromatography Phaseolus lunatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 seed dry large lima bean seeds Phaseolus lunatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 49.6
-
 purified native enzyme Phaseolus lunatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
-
 Phaseolus lunatus ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Phaseolus lunatus ?
-
 ?
3.2.1.14 additional information the enzyme exerts a potent inhibitory action toward fungal species, including Fusarium solani, Pythium aphanidermatum, and Sclerotium rolfsii Phaseolus lunatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 monomer 1 * 28600, SDS-PAGE Phaseolus lunatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 40 50
-
 Phaseolus lunatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 30 80 temperature profile, overview Phaseolus lunatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 55
-
 stable up to Phaseolus lunatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5.4
-
-
 Phaseolus lunatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 4 8 pH profile, overview Phaseolus lunatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Phaseolus lunatus isoelectric focusing
-
 7.8