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Literature summary extracted from

  • Silvente, S.; Reddy, P.M.; Khandual, S.; Blanco, L.; Alvarado-Affantranger, X.; Sanchez, F.; Lara-Flores, M.
    Evidence for sugar signalling in the regulation of asparagine synthetase gene expressed in Phaseolus vulgaris roots and nodules (2008), J. Exp. Bot., 59, 1279-1294.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.4 additional information addition of sugars to the plants, mainly glucose, induces the enzymes leading to the increased asparagine production Phaseolus vulgaris

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.4 gene NAS2, DNA and mino acid sequence determination and analysis, promoter determination, tissue expression analysis, overview Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.4 additional information PvNAS2 is downregulated when carbon availability is reduced in nodules Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.4 Mg2+
-
 Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.4 ATP + L-aspartate + L-glutamine + H2O Phaseolus vulgaris
-
 AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
6.3.5.4 additional information Phaseolus vulgaris role for hexokinase in the sugar-sensing mechanism that regulates PvNAS2 expression in roots, downregulation of the asparagine synthetase enzyme and concomitantly asparagine production. Thereby a favourable environment is created for the efficient transfer of the amido group of glutamine for the synthesis of purines, and then ureide generation. ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Phaseolus vulgaris
-
-
-
6.3.5.4 Phaseolus vulgaris A9XS73 cv. Negro Jamapa, gene NAS2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.5.4 root expression of PvNAS2 in roots is confined to vascular bundles and meristematic tissues, while in root nodules its expression is solely localized to vascular traces and outer cortical cells encompassing the central nitrogen-fixing zone, but never detected in either infected or non-infected cells located in the central region of the nodule Phaseolus vulgaris
-
6.3.5.4 root high expression, confined to vascular bundles and meristematic tissues Phaseolus vulgaris
-
6.3.5.4 root nodule expression of PvNAS2 in roots is confined to vascular bundles and meristematic tissues, while in root nodules its expression is solely localized to vascular traces and outer cortical cells encompassing the central nitrogen-fixing zone, but never detected in either infected or non-infected cells located in the central region of the nodule Phaseolus vulgaris
-
6.3.5.4 root nodule mainly induced during the early days of nitrogen fixation, confined to vascular traces and outer cortical cells. Down-regulation when carbon availability is reduced, while the addition of sugars to the plants induces expression Phaseolus vulgaris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-aspartate + L-glutamine + H2O
-
 Phaseolus vulgaris AMP + diphosphate + L-asparagine + L-glutamate
-
 ?
6.3.5.4 additional information role for hexokinase in the sugar-sensing mechanism that regulates PvNAS2 expression in roots, downregulation of the asparagine synthetase enzyme and concomitantly asparagine production. Thereby a favourable environment is created for the efficient transfer of the amido group of glutamine for the synthesis of purines, and then ureide generation. Phaseolus vulgaris ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.5.4 asparagine amidotransferase
-
 Phaseolus vulgaris
6.3.5.4 Asparagine synthetase
-
 Phaseolus vulgaris
6.3.5.4 NAS2
-
 Phaseolus vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.5.4 ATP
-
 Phaseolus vulgaris