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Literature summary extracted from

  • Allardyce, B.J.; Linton, S.M.
    Purification and characterisation of endo-beta-1,4-glucanase and laminarinase enzymes from the gecarcinid land crab Gecarcoidea natalis and the aquatic crayfish Cherax destructor (2008), J. Exp. Biol., 211, 2275-2287.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 51000
-
 SDS-PAGE Cherax destructor
3.2.1.4 52000
-
-
 Gecarcoidea natalis
3.2.1.4 52000
-
 SDS-PAGE Gecarcoidea natalis
3.2.1.4 52000
-
 endo-beta-1,4-glucanase 2 Cherax destructor
3.2.1.4 53000
-
 SDS-PAGE Cherax destructor
3.2.1.4 53000
-
 endo-beta-1,4-glucanase 1 Cherax destructor
3.2.1.6 41000
-
 SDS-PAGE, a second band of 62000 Da is detected Cherax destructor
3.2.1.6 62000
-
 gel filtration chromatography Cherax destructor
3.2.1.6 62000
-
 SDS-PAGE, a second band of 41000 Da is detected, the larger band may either be a minor impurity or represent a native laminarinase dimer Cherax destructor
3.2.1.39 41000
-
 SDS-PAGE Gecarcoidea natalis
3.2.1.39 71000
-
 gel filtration chromatography Gecarcoidea natalis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Cherax destructor
-
-
-
3.2.1.4 Cherax destructor Clark 1936
-
-
-
3.2.1.4 Gecarcoidea natalis
-
-
-
3.2.1.4 Gecarcoidea natalis Pocock 1888
-
-
-
3.2.1.6 Cherax destructor
-
-
-
3.2.1.6 Cherax destructor Clark 1936
-
-
-
3.2.1.39 Gecarcoidea natalis
-
-
-
3.2.1.39 Gecarcoidea natalis Pocock 1888
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 gel filtration Cherax destructor
3.2.1.4 gel filtration Gecarcoidea natalis
3.2.1.4 precipitation, a combination of anion exchange, hydrophobic interaction and gel filtration chromatography, all chromatography is carried out at 4°C Cherax destructor
3.2.1.4 precipitation, a combination of anion exchange, hydrophobic interaction and gel filtration chromatography, all chromatography is carried out at 4°C Gecarcoidea natalis
3.2.1.6 precipitation, a combination of anion exchange, hydrophobic interaction and gel filtration chromatography, all chromatography is carried out at 4°C Cherax destructor
3.2.1.39 precipitation, a combination of anion exchange, hydrophobic interaction and gel filtration chromatography, all chromatography is carried out at 4°C Gecarcoidea natalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.4 midgut
-
 Cherax destructor
-
3.2.1.4 midgut
-
 Gecarcoidea natalis
-
3.2.1.4 midgut gland
-
 Cherax destructor
-
3.2.1.4 midgut gland
-
 Gecarcoidea natalis
-
3.2.1.6 midgut
-
 Cherax destructor
-
3.2.1.39 midgut
-
 Gecarcoidea natalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 0.023
-
 homogenate Gecarcoidea natalis
3.2.1.4 0.166
-
 after DEAE anion exchange chromatography Gecarcoidea natalis
3.2.1.4 0.23
-
 homogenate Cherax destructor
3.2.1.4 5.13
-
 after DEAE anion exchange chromatography Cherax destructor
3.2.1.4 7.43
-
 after gel filtration chromatography Cherax destructor
3.2.1.4 11.61
-
 after DEAE anion exchange chromatography Cherax destructor
3.2.1.4 12.74
-
 last purification step Gecarcoidea natalis
3.2.1.4 12.74
-
 after gel filtration chromatography Gecarcoidea natalis
3.2.1.4 19.09
-
 last purification step Cherax destructor
3.2.1.4 19.09
-
 after gel filtration chromatography Cherax destructor
3.2.1.6 0.17
-
 homogenate Cherax destructor
3.2.1.6 1.32
-
 DEAE anion exchange chromatography Cherax destructor
3.2.1.6 6.37
-
 HIC chromatography Cherax destructor
3.2.1.6 18.31
-
 gel filtration chromatography Cherax destructor
3.2.1.39 0.096
-
 homogenate Gecarcoidea natalis
3.2.1.39 0.252
-
 after DEAE anion exchange chromatography Gecarcoidea natalis
3.2.1.39 0.945
-
 after HIC chromatography Gecarcoidea natalis
3.2.1.39 7.8
-
 after gel filtration chromatography Gecarcoidea natalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 Cherax destructor D-glucose + cellobiose + cellooligosaccharide
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Cherax destructor Clark 1936 D-glucose + cellobiose + cellooligosaccharide
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Gecarcoidea natalis glucose + cellobiose + short oligomers
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Gecarcoidea natalis Pocock 1888 glucose + cellobiose + short oligomers
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Cherax destructor additional information short glucose oligomers, cellobiose and a very small amount of glucose ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Cherax destructor Clark 1936 additional information short glucose oligomers, cellobiose and a very small amount of glucose ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Gecarcoidea natalis short glucose oligomers + cellobiose + glucose
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Gecarcoidea natalis Pocock 1888 short glucose oligomers + cellobiose + glucose
-
 ?
3.2.1.4 additional information glucanase hydrolyses beta-1,4-glycosidic bonds Cherax destructor ?
-
 ?
3.2.1.4 additional information glucanase hydrolyses mainly beta-1,4-glycosidic bonds but is also capable of significant hydrolysis of beta-1,3-glycosidic bonds Gecarcoidea natalis ?
-
 ?
3.2.1.4 additional information glucanase hydrolyses mainly beta-1,4-glycosidic bonds but is also capable of significant hydrolysis of beta-1,3-glycosidic bonds Gecarcoidea natalis Pocock 1888 ?
-
 ?
3.2.1.4 additional information glucanase hydrolyses beta-1,4-glycosidic bonds Cherax destructor Clark 1936 ?
-
 ?
3.2.1.6 laminarin + H2O
-
 Cherax destructor D-glucose + laminarioligosaccharides
-
 ?
3.2.1.6 laminarin + H2O
-
 Cherax destructor Clark 1936 D-glucose + laminarioligosaccharides
-
 ?
3.2.1.6 additional information laminarinase hydrolyses both beta-1,3-glycosidic bonds and beta-1,4-glycosidic bonds Cherax destructor ?
-
 ?
3.2.1.6 additional information laminarinase hydrolyses both beta-1,3-glycosidic bonds and beta-1,4-glycosidic bonds Cherax destructor Clark 1936 ?
-
 ?
3.2.1.39 laminarin + H2O
-
 Gecarcoidea natalis D-glucose + laminarioligosaccharides
-
 ?
3.2.1.39 laminarin + H2O
-
 Gecarcoidea natalis Pocock 1888 D-glucose + laminarioligosaccharides
-
 ?
3.2.1.39 additional information laminarinase mainly hydrolyses beta?1,3-glycosidic bonds, with negligible activity towards beta-1,4-glycosidic bonds Gecarcoidea natalis ?
-
 ?
3.2.1.39 additional information laminarinase mainly hydrolyses beta?1,3-glycosidic bonds, with negligible activity towards beta-1,4-glycosidic bonds Gecarcoidea natalis Pocock 1888 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 More 41000 SDS-PAGE, 71000 gel filtration chromatography, lamarinase is a dimer or contains a laminarin-binding domain, which is in similar size to the catalytic compound Gecarcoidea natalis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 endo-1,4-beta-glucanase 1
-
 Cherax destructor
3.2.1.4 endo-1,4-beta-glucanase 2
-
 Cherax destructor
3.2.1.4 endo-beta-1,4-glucanase
-
 Gecarcoidea natalis
3.2.1.4 endo-beta-1,4-glucanase 1
-
 Cherax destructor
3.2.1.4 endo-beta-1,4-glucanase 2
-
 Cherax destructor
3.2.1.6 endo-1,3-beta-glucanase
-
 Cherax destructor
3.2.1.6 lamarinase
-
 Cherax destructor
3.2.1.39 endo-1,3-beta-glucanase
-
 Gecarcoidea natalis
3.2.1.39 laminarinase
-
 Gecarcoidea natalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4 7
-
 Gecarcoidea natalis
3.2.1.4 5.5
-
 endo-beta-1,4-glucanase 1 Cherax destructor
3.2.1.6 5.5
-
-
 Cherax destructor
3.2.1.39 7
-
-
 Gecarcoidea natalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 4 7
-
 Gecarcoidea natalis
3.2.1.6 5.5 8
-
 Cherax destructor
3.2.1.39 5.5 8
-
 Gecarcoidea natalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.6 5.5 8
-
 Cherax destructor
3.2.1.39 5.5 8
-
 Gecarcoidea natalis