EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.1.6 | expressed in Escherichia coli ER2566 cells | Acetivibrio thermocellus |
5.3.1.6 | gene rpiB, expression in Escherichia coli strain ER2566 | Acetivibrio thermocellus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.6 | additional information | - |
additional information | kinetics with aldose and ketose substrates, recombinant enzyme, overview | Acetivibrio thermocellus | |
5.3.1.6 | 0.015 | - |
D-ribulose 5-phosphate | pH 7.5, 65°C, recombinant enzyme | Acetivibrio thermocellus | |
5.3.1.6 | 0.017 | - |
D-ribose 5-phosphate | pH 7.5, 65°C, recombinant enzyme | Acetivibrio thermocellus | |
5.3.1.6 | 15 | - |
D-ribulose 5-phosphate | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 17 | - |
D-ribose 5-phosphate | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 34 | - |
D-ribulose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 37 | - |
L-talose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 44 | - |
D-ribose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 53 | - |
D-psicose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 98 | - |
L-allose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 106 | - |
D-allose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 125 | - |
L-tagatose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 173 | - |
L-ribose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 232 | - |
D-talose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 319 | - |
L-ribulose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 17000 | - |
2 * 17000, gel filtration | Acetivibrio thermocellus |
5.3.1.6 | 34000 | - |
gel filtration | Acetivibrio thermocellus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.6 | D-ribose 5-phosphate | Acetivibrio thermocellus | preferred substrate | D-ribulose 5-phosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.6 | Acetivibrio thermocellus | - |
- |
- |
5.3.1.6 | Acetivibrio thermocellus | - |
RpiB; gene rpiB | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.1.6 | HisTrap HP column chromatography | Acetivibrio thermocellus |
5.3.1.6 | recombinant RpiB from Escherichia coli strain ER2566 by His affinity chromatography | Acetivibrio thermocellus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 0.21 | - |
purified enzyme, using D-tagatose as substrate, at 65°C and pH 7.5 | Acetivibrio thermocellus |
5.3.1.6 | 0.53 | - |
purified enzyme, using L-allose as substrate, at 65°C and pH 7.5 | Acetivibrio thermocellus |
5.3.1.6 | 12 | - |
purified enzyme, using L-psicose as substrate, at 65°C and pH 7.5 | Acetivibrio thermocellus |
5.3.1.6 | 50 | - |
purified enzyme, using D-talose as substrate, at 65°C and pH 7.5 | Acetivibrio thermocellus |
5.3.1.6 | 272 | - |
purified enzyme, using L-ribose as substrate, at 65°C and pH 7.5 | Acetivibrio thermocellus |
5.3.1.6 | 600 | - |
purified enzyme, using L-allose as substrate, at 65°C and pH 7.5 | Acetivibrio thermocellus |
5.3.1.6 | 720 | - |
purified enzyme, using L-ribulose as substrate, at 65°C and pH 7.5 | Acetivibrio thermocellus |
5.3.1.6 | 1037 | - |
purified enzyme, using D-psicose as substrate, at 65°C and pH 7.5 | Acetivibrio thermocellus |
5.3.1.6 | 1037 | - |
purified recombinant enzyme, substrate D-psicose | Acetivibrio thermocellus |
5.3.1.6 | 1352 | - |
purified enzyme, using D-allose as substrate, at 65°C and pH 7.5 | Acetivibrio thermocellus |
5.3.1.6 | 1352 | - |
purified recombinant enzyme, substrate D-allose | Acetivibrio thermocellus |
5.3.1.6 | 5374 | - |
purified enzyme, using D-ribulose as substrate, at 65°C and pH 7.5 | Acetivibrio thermocellus |
5.3.1.6 | 5374 | - |
purified recombinant enzyme, substrate D-ribulose | Acetivibrio thermocellus |
5.3.1.6 | 5800 | - |
purified enzyme, using D-ribose as substrate, at 65°C and pH 7.5 | Acetivibrio thermocellus |
5.3.1.6 | 5800 | - |
purified recombinant enzyme, substrate D-ribose | Acetivibrio thermocellus |
5.3.1.6 | 7363 | - |
purified enzyme, using L-talose as substrate, at 65°C and pH 7.5 | Acetivibrio thermocellus |
5.3.1.6 | 7363 | - |
purified recombinant enzyme, substrate L-talose | Acetivibrio thermocellus |
5.3.1.6 | 19680 | - |
purified enzyme, using D-ribulose 5-phosphate as substrate, at 65°C and pH 7.5 | Acetivibrio thermocellus |
5.3.1.6 | 19680 | - |
purified recombinant enzyme, substrate D-ribulose 5-phosphate | Acetivibrio thermocellus |
5.3.1.6 | 25690 | - |
purified enzyme, using D-ribose 5-phosphate as substrate, at 65°C and pH 7.5 | Acetivibrio thermocellus |
5.3.1.6 | 25690 | - |
purified recombinant enzyme, substrate D-ribose 5-phosphate | Acetivibrio thermocellus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.6 | D-allose | - |
Acetivibrio thermocellus | D-psicose | - |
r | |
5.3.1.6 | D-ribose | - |
Acetivibrio thermocellus | D-ribulose | 38% conversion to D-ribose 5-phosphate yield after approximately 90 min | r | |
5.3.1.6 | D-ribose | 39% conversion yield after approximately 90 min | Acetivibrio thermocellus | D-ribulose | - |
r | |
5.3.1.6 | D-ribose 5-phosphate | - |
Acetivibrio thermocellus | D-ribulose 5-phosphate | - |
r | |
5.3.1.6 | D-ribose 5-phosphate | preferred substrate | Acetivibrio thermocellus | D-ribulose 5-phosphate | - |
r | |
5.3.1.6 | D-ribose 5-phosphate | preferred substrate | Acetivibrio thermocellus | D-ribulose 5-phosphate | 38% conversion to D-ribose 5-phosphate yield after approximately 90 min at 50°C | r | |
5.3.1.6 | D-talose | - |
Acetivibrio thermocellus | D-tagatose | - |
r | |
5.3.1.6 | L-allose | - |
Acetivibrio thermocellus | L-psicose | - |
r | |
5.3.1.6 | L-ribose | - |
Acetivibrio thermocellus | L-ribulose | - |
r | |
5.3.1.6 | L-talose | 89% conversion yield after approximately 90 min | Acetivibrio thermocellus | L-tagatose | - |
r | |
5.3.1.6 | L-talose | the enzyme prefers the forward reaction, high activity, 89% conversion to L-tagatose yield after approximately 90 min at 50°C | Acetivibrio thermocellus | L-tagatose | - |
r | |
5.3.1.6 | additional information | RpiB also displays activity with L-talose, D-ribose, D-allose, L-allose, L-ribose, and D-talose in decreasing order. The enzyme shows specificity for aldose substrates possessing hydroxyl groups oriented in the same direction at the C2, C3, and C4 positions. The substrate specificity through substrate interactions with residues Tyr42, His98, and His9, which interact with the hydroxyl groups of C2, C3, and C4, respectively, oriented in the same direction, homology molecular modeling, overview | Acetivibrio thermocellus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.1.6 | homodimer | 2 * 17000, gel filtration | Acetivibrio thermocellus |
5.3.1.6 | More | homology modeling of RpiB, substrate binding structure, overview | Acetivibrio thermocellus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.1.6 | D-ribose-5-phosphate isomerase | - |
Acetivibrio thermocellus |
5.3.1.6 | RpiB | - |
Acetivibrio thermocellus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 65 | - |
assay at | Acetivibrio thermocellus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.6 | 17 | - |
L-ribulose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 119 | - |
D-talose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 322 | - |
L-ribose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 1506 | - |
L-allose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 1822 | - |
L-tagatose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 2347 | - |
D-psicose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 2682 | - |
D-allose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 9193 | - |
D-ribulose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 11880 | - |
D-ribose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 13420 | - |
L-talose | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 39530 | - |
D-ribulose 5-phosphate | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 39530 | - |
D-ribulose 5-phosphate | pH 7.5, 65°C, recombinant enzyme | Acetivibrio thermocellus | |
5.3.1.6 | 52000 | - |
D-ribose 5-phosphate | in 50 mM Tris-HCl buffer (pH 7.5), at 65°C | Acetivibrio thermocellus | |
5.3.1.6 | 52000 | - |
D-ribose 5-phosphate | pH 7.5, 65°C, recombinant enzyme | Acetivibrio thermocellus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.1.6 | 7.5 | - |
assay at | Acetivibrio thermocellus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.6 | 2589 | - |
D-ribulose 5-phosphate | pH 7.5, 65°C, recombinant enzyme | Acetivibrio thermocellus | |
5.3.1.6 | 3029 | - |
D-ribose 5-phosphate | pH 7.5, 65°C, recombinant enzyme | Acetivibrio thermocellus |