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Literature summary extracted from

  • Schrenkhammer, P.; Rosnizeck, I.C.; Duerkop, A.; Wolfbeis, O.S.; Schaeferling, M.
    Time-resolved fluorescence-based assay for the determination of alkaline phosphatase activity and application to the screening of its inhibitors (2008), J. Biomol. Screen., 13, 9-16.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.1 biotechnology an assay is developed for determination of the activity of the ALP using the effect of enhancement of fluorescence of the europium(III)-tetracycline 3:1 complex (Eu(3)TC). Its luminescence, peaking at 616 nm if excited at 405 nm, is enhanced by a factor of 2.5 in the presence of phosphate. Phosphate coordinates to Eu(3)TC and enhances its luminescence intensity as a result of the displacement of water from the inner coordination sphere of the central metal. The assay is performed in a time-resolved (gated) mode, which is shown to yield larger signal changes than steady-state measurement of fluorescence. The limit of detection for ALP is 4 micromol/l. Based on this scheme, a model assay for theophylline as inhibitor for ALP is developed with a linear range from 14 to 68 micromol/l of theophylline Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 theophylline
-
 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Bos taurus P19111
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.1 intestine
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Bos taurus 4-nitrophenol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.1 alkaline phosphatase
-
 Bos taurus
3.1.3.1 ALP
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 25
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 8
-
 assay at Bos taurus