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Literature summary extracted from

  • Wang, Y.C.; Wang, S.T.; Li, C.; Chen, L.Y.; Liu, W.H.; Chen, P.R.; Chou, M.C.; Liu, T.C.
    The role of amino acids T148 and R281 in human dihydrolipoamide dehydrogenase (2008), J. Biomed. Sci., 15, 37-46.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.8.1.4 degradation T148 is not important to E3 catalytic function, whereas R281 plays a role in the catalytic function of E3 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.4 mutants cloned into pQE-9 vector Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.4 R281K specific activity is 11.93% to that of wild-type E3. FAD-content is about 93% that of wild-type E3. Kcat of forward reaction is decreased dramatically. Substitution has no effect in the self-dimerization Homo sapiens
1.8.1.4 R281N specific activity is 12.50% to that of wild-type E3. FAD-content is about 96% that of wild-type E3. Kcat of forward reaction is decreased dramatically. Substitution has no effect in the self-dimerization Homo sapiens
1.8.1.4 T148G specific activity is 76.34% to that of wild-type E3. FAD-content is about 710% that of wild-type E3. Substitution has no effect in the self-dimerization Homo sapiens
1.8.1.4 T148S specific activity is 88.62% to that of wild-type E3. FAD-content is about 92% that of wild-type E3. Substitution has no effect in the self-dimerization Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.4 0.12
-
 dihydrolipoamide mutant R281K Homo sapiens
1.8.1.4 0.22
-
 NAD+ wild-type E3 Homo sapiens
1.8.1.4 0.28
-
 NAD+ mutant T148G Homo sapiens
1.8.1.4 0.29
-
 NAD+ mutant T148S Homo sapiens
1.8.1.4 0.38
-
 dihydrolipoamide mutant R281N Homo sapiens
1.8.1.4 0.38
-
 dihydrolipoamide wild-type E3 Homo sapiens
1.8.1.4 0.43
-
 NAD+ mutant R281K Homo sapiens
1.8.1.4 0.5
-
 NAD+ mutant R281N Homo sapiens
1.8.1.4 0.58
-
 dihydrolipoamide mutant T148G Homo sapiens
1.8.1.4 0.59
-
 dihydrolipoamide mutant T148S Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.4 110000
-
 molecular sieving HPLC Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Homo sapiens P09622
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4 recombinant His-tagged E3 wild-type and mutant proteins Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 dihydrolipoamide + NAD+
-
 Homo sapiens lipoamide + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.8.1.4 dimer molecular sieving HPLC Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 dihydrolipoamide dehydrogenase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.4 220
-
 dihydrolipoamide mutant R281N Homo sapiens
1.8.1.4 223
-
 dihydrolipoamide mutant R281K Homo sapiens
1.8.1.4 505
-
 dihydrolipoamide wild-type E3 Homo sapiens
1.8.1.4 655
-
 dihydrolipoamide mutant T148G Homo sapiens
1.8.1.4 742
-
 dihydrolipoamide mutant T148S Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 FAD
-
 Homo sapiens