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Literature summary extracted from

  • Zhang, L.; Bharadwaj, A.G.; Casper, A.; Barkley, J.; Barycki, J.J.; Simpson, M.A.
    Hyaluronidase activity of human hyal1 requires active site acidic and tyrosine residues (2009), J. Biol. Chem., 284, 9433-9442.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.35 expressed in 22Rv1 human prostate adenomacarcinoma cells and in HEK-293 human embryonic kidney cells as a N-terminal FLAG fusion protein. Abundant soluble FLAG-tagged Hyal1 is secreted to the conditioned cell culture media of transfectants Homo sapiens
3.2.1.35 FLAG-tag Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.35 D129N catalytic mutant, catalysis impaired Homo sapiens
3.2.1.35 D129N catalytic mutant, Km higher compared to wild-type, Vmax lower than wild-type Homo sapiens
3.2.1.35 DELTA351-end putative structural mutant with a deleted EGF-like domain, significant decreased enzymatic activity Homo sapiens
3.2.1.35 DELTA356-end putative structural mutant with a deleted EGF-like domain, significant decreased enzymatic activity Homo sapiens
3.2.1.35 E131Q catalytic mutant, activity eliminated Homo sapiens
3.2.1.35 E131Q catalytic mutant, no measurable activity Homo sapiens
3.2.1.35 L356tr putative structural mutant, reduced activity Homo sapiens
3.2.1.35 N216A putative structural mutant, reduced activity Homo sapiens
3.2.1.35 N216A putative structural mutant, Km higher compared to wild-type, Vmax unaltered compared to wild-type, ca. 44% of wild-type activity Homo sapiens
3.2.1.35 N350A putative structural mutant, reduced activity Homo sapiens
3.2.1.35 N350A putative structural mutant, significant decreased enzymatic activity Homo sapiens
3.2.1.35 N350tr putative structural mutant, reduced activity Homo sapiens
3.2.1.35 R265L putative structural mutant, reduced activity Homo sapiens
3.2.1.35 R265L putative structural mutant, significant decreased enzymatic activity Homo sapiens
3.2.1.35 S245A substrate binding mutant, reduced activity Homo sapiens
3.2.1.35 S245A substrate binding mutant, Km higher compared to wild-type, Vmax unaltered compared to wild-type, ca. 41% of wild-type activity Homo sapiens
3.2.1.35 Y202F substrate binding mutant, catalysis impaired Homo sapiens
3.2.1.35 Y202F substrate binding mutant, Km higher compared to wild-type, Vmax lower than wild-type Homo sapiens
3.2.1.35 Y247F catalytic mutant, activity eliminated Homo sapiens
3.2.1.35 Y247F catalytic mutant, no measurable activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.35 0.038
-
 hyaluronan Hyal1 wild-type Homo sapiens
3.2.1.35 0.0381
-
 hyaluronan wild-type, Vmax: 12.5 micromol/min/mg Homo sapiens
3.2.1.35 0.103
-
 hyaluronan Hyal1 mutant N216A Homo sapiens
3.2.1.35 0.103
-
 hyaluronan mutant N216A, Vmax: 10 micromol/min/mg Homo sapiens
3.2.1.35 0.11
-
 hyaluronan Hyal1 mutant S245A Homo sapiens
3.2.1.35 0.11
-
 hyaluronan mutant S245A, Vmax: 10.7 micromol/min/mg Homo sapiens
3.2.1.35 0.181
-
 hyaluronan Hyal1 mutant D129N Homo sapiens
3.2.1.35 0.181
-
 hyaluronan mutant D129N, Vmax: 1.9 micromol/min/mg Homo sapiens
3.2.1.35 0.367
-
 hyaluronan Hyal1 mutant Y202F Homo sapiens
3.2.1.35 0.367
-
 hyaluronan mutant Y202F, Vmax: 6.7 micromol/min/mg Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Homo sapiens
-
-
-
3.2.1.35 Homo sapiens Q12794
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 hyaluronan + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 HYAL1
-
 Homo sapiens
3.2.1.35 hyaluronidase
-
 Homo sapiens
3.2.1.35 hyaluronidase isoenzyme Hyal1 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.35 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 9.6
-
 assay at Homo sapiens