Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Race, P.R.; Bentley, M.L.; Melvin, J.A.; Crow, A.; Hughes, R.K.; Smith, W.D.; Sessions, R.B.; Kehoe, M.A.; McCafferty, D.G.; Banfield, M.J.
    Crystal structure of Streptococcus pyogenes sortase A: implications for sortase mechanism (2009), J. Biol. Chem., 284, 6924-6933.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.70 DNA sequence encoding SrtA residues Val82-Thr249, expression in Escherichia coli BL21 Streptococcus pyogenes

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.22.70 hanging drop method of vapor diffusion at 20°C, SrtA residues Val82-Thr249 (catalytic domain) Streptococcus pyogenes

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.70 Streptococcus pyogenes Q99ZN4
-
-
3.4.22.70 Streptococcus pyogenes SF370 Q99ZN4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.70 recombinant enzyme, SrtA residues Val82-Thr249 (catalytic domain) Streptococcus pyogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.70 Abz-LPETGG-Dap(Dnp)-NH2 + Ala-Ala transpeptidation of LPXTG-containing peptides to the cell-wall precursor mimic AlaAla Streptococcus pyogenes Abz-LPETAA + GG-Dap(Dnp)-NH2
-
 ?
3.4.22.70 Abz-LPETGG-Dap(Dnp)-NH2 + Ala-Ala transpeptidation of LPXTG-containing peptides to the cell-wall precursor mimic AlaAla Streptococcus pyogenes SF370 Abz-LPETAA + GG-Dap(Dnp)-NH2
-
 ?