BRENDA - Enzyme Database

Lipin 2 is a liver-enriched phosphatidate phosphohydrolase enzyme that is dynamically regulated by fasting and obesity in mice

Gropler, M.C.; Harris, T.E.; Hall, A.M.; Wolins, N.E.; Gross, R.W.; Han, X.; Chen, Z.; Finck, B.N.; J. Biol. Chem. 284, 6763-6772 (2009)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
additional information
hepatic lipin 2 protein content is markedly increased by lipin 1 deficiency, food deprivation, and obesity, often independent of changes in steady-state mRNA levels. Fasting induces expression of Lpin1 and Lpin2 in wild-type and PGC-1alpha-deficient mice
Mus musculus
Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
expression analysis of lipin-1 and lipin-2, recombinant expression of lipin-2 in Hep-G2 cells using an adenoviral vector
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
livers of fld mice lacking lipin 1 exhibit normal PAP-1 activity and a 20fold increase in triglyceride levels, while the hepatic content of phosphatidate is markedly diminished in fld mice. Other phospholipids derived from phosphatidate, phosphatidylglycerol and cardiolipin, are also depleted. Hepatic lipin 2 protein content is markedly increased by lipin 1 deficiency via a posttranscriptional mechanism
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
lipin-1 and lipin-2 are soluble proteins that can also be localized to the nucleus or associate with endoplasmic reticulum membranes
Mus musculus
5829
-
3.1.3.4
endoplasmic reticulum membrane
lipin-1 and lipin-2 are soluble proteins that can also be localized to the nucleus or associate with endoplasmic reticulum membranes
Mus musculus
5789
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
required
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
a 3-sn-phosphatidate + H2O
Mus musculus
lipin 1 is a bifunctional intracellular protein that regulates fatty acid metabolism in the nucleus via interactions with DNA-bound transcription factors and at the endoplasmic reticulum as a phosphatidic acid phosphohydrolase enzyme to catalyze the penultimate step in triglyceride synthesis. Lipin 2 plays an important role as a hepatic PAP-1 enzyme
a 1,2-diacyl-sn-glycerol + phosphate
-
-
?
3.1.3.4
additional information
Mus musculus
lipin 2 is dynamically regulated in liver but is not a target gene of PGC-1alpha
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Mus musculus
-
genes Lpin1 and Lpin2
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.1.3.4
phosphoprotein
serine 106 residue of lipin-1 and lipin-2 is a major site of phosphorylation
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
liver
lipin-1 and high activity of lipin-2
Mus musculus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.3.4
additional information
-
-
Mus musculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
a 3-sn-phosphatidate + H2O
-
693251
Mus musculus
a 1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
a 3-sn-phosphatidate + H2O
lipin 1 is a bifunctional intracellular protein that regulates fatty acid metabolism in the nucleus via interactions with DNA-bound transcription factors and at the endoplasmic reticulum as a phosphatidic acid phosphohydrolase enzyme to catalyze the penultimate step in triglyceride synthesis. Lipin 2 plays an important role as a hepatic PAP-1 enzyme
693251
Mus musculus
a 1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
additional information
lipin 2 is dynamically regulated in liver but is not a target gene of PGC-1alpha
693251
Mus musculus
?
-
-
-
-
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
3.1.3.4
30
-
assay at
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
7
-
assay at
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.4
additional information
hepatic lipin 2 protein content is markedly increased by lipin 1 deficiency, food deprivation, and obesity, often independent of changes in steady-state mRNA levels. Fasting induces expression of Lpin1 and Lpin2 in wild-type and PGC-1alpha-deficient mice
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
expression analysis of lipin-1 and lipin-2, recombinant expression of lipin-2 in Hep-G2 cells using an adenoviral vector
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
livers of fld mice lacking lipin 1 exhibit normal PAP-1 activity and a 20fold increase in triglyceride levels, while the hepatic content of phosphatidate is markedly diminished in fld mice. Other phospholipids derived from phosphatidate, phosphatidylglycerol and cardiolipin, are also depleted. Hepatic lipin 2 protein content is markedly increased by lipin 1 deficiency via a posttranscriptional mechanism
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
lipin-1 and lipin-2 are soluble proteins that can also be localized to the nucleus or associate with endoplasmic reticulum membranes
Mus musculus
5829
-
3.1.3.4
endoplasmic reticulum membrane
lipin-1 and lipin-2 are soluble proteins that can also be localized to the nucleus or associate with endoplasmic reticulum membranes
Mus musculus
5789
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
required
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
a 3-sn-phosphatidate + H2O
Mus musculus
lipin 1 is a bifunctional intracellular protein that regulates fatty acid metabolism in the nucleus via interactions with DNA-bound transcription factors and at the endoplasmic reticulum as a phosphatidic acid phosphohydrolase enzyme to catalyze the penultimate step in triglyceride synthesis. Lipin 2 plays an important role as a hepatic PAP-1 enzyme
a 1,2-diacyl-sn-glycerol + phosphate
-
-
?
3.1.3.4
additional information
Mus musculus
lipin 2 is dynamically regulated in liver but is not a target gene of PGC-1alpha
?
-
-
-
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.1.3.4
phosphoprotein
serine 106 residue of lipin-1 and lipin-2 is a major site of phosphorylation
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
liver
lipin-1 and high activity of lipin-2
Mus musculus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
3.1.3.4
additional information
-
-
Mus musculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
a 3-sn-phosphatidate + H2O
-
693251
Mus musculus
a 1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
a 3-sn-phosphatidate + H2O
lipin 1 is a bifunctional intracellular protein that regulates fatty acid metabolism in the nucleus via interactions with DNA-bound transcription factors and at the endoplasmic reticulum as a phosphatidic acid phosphohydrolase enzyme to catalyze the penultimate step in triglyceride synthesis. Lipin 2 plays an important role as a hepatic PAP-1 enzyme
693251
Mus musculus
a 1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
additional information
lipin 2 is dynamically regulated in liver but is not a target gene of PGC-1alpha
693251
Mus musculus
?
-
-
-
-
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
3.1.3.4
30
-
assay at
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
7
-
assay at
Mus musculus