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Literature summary extracted from

  • Nakajima, M.; Nishimoto, M.; Kitaoka, M.
    Characterization of three beta-galactoside phosphorylases from clostridium phytofermentans: discovery of D-galactosyl-beta1,4-L-rhamnose phosphorylase (2009), J. Biol. Chem., 284, 19220-19227.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.211 synthesis suitable catalyst for practical syntheses of oligosaccharides Lachnoclostridium phytofermentans
2.4.1.247 synthesis suitable catalyst for practical syntheses of oligosaccharides Lachnoclostridium phytofermentans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.211 expressed in Escherichia coli BL21(DE3) Lachnoclostridium phytofermentans
2.4.1.247 expressed in Escherichia coli BL21(DE3) Lachnoclostridium phytofermentans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.211 1.9
-
 N-acetyl-D-glucosamine
-
 Lachnoclostridium phytofermentans
2.4.1.211 2.8
-
 lacto-N-biose I
-
 Lachnoclostridium phytofermentans
2.4.1.211 3.3
-
 N-acetyl-D-galactosamine
-
 Lachnoclostridium phytofermentans
2.4.1.211 3.4
-
 N-acetyl-D-glucosamine
-
 Lachnoclostridium phytofermentans
2.4.1.211 4.5
-
 lacto-N-biose I
-
 Lachnoclostridium phytofermentans
2.4.1.211 8
-
 galacto-N-biose
-
 Lachnoclostridium phytofermentans
2.4.1.211 10
-
 galacto-N-biose
-
 Lachnoclostridium phytofermentans
2.4.1.247 2.4
-
 L-rhamnose
-
 Lachnoclostridium phytofermentans
2.4.1.247 7.9
-
 D-galactosyl-beta-1,4-L-rhamnose
-
 Lachnoclostridium phytofermentans
2.4.1.247 78
-
 L-Mannose
-
 Lachnoclostridium phytofermentans
2.4.1.247 220
-
 L-Lyxose
-
 Lachnoclostridium phytofermentans
2.4.1.247 290
-
 2-deoxy-D-glucose
-
 Lachnoclostridium phytofermentans
2.4.1.247 460
-
 D-glucose
-
 Lachnoclostridium phytofermentans
2.4.1.247 610
-
 D-galactose
-
 Lachnoclostridium phytofermentans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.211 cytosol proteins are predicted not to possess N-terminal signal peptides Lachnoclostridium phytofermentans 5829
-
2.4.1.247 cytosol proteins are predicted not to possess N-terminal signal peptides Lachnoclostridium phytofermentans 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.211 80000
-
 SDS-PAGE Lachnoclostridium phytofermentans
2.4.1.211 84860
-
 calculated Lachnoclostridium phytofermentans
2.4.1.211 84940
-
 calculated Lachnoclostridium phytofermentans
2.4.1.247 80000
-
 SDS-PAGE Lachnoclostridium phytofermentans
2.4.1.247 84230
-
 calculated Lachnoclostridium phytofermentans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.211 Lachnoclostridium phytofermentans A9KIW5 bacterium possesses three GalHexNAcP homologous genes, cphy0577 gene; anaerobic cellulolytic bacterium
-
2.4.1.211 Lachnoclostridium phytofermentans A9KQ75 bacterium possesses three GalHexNAcP homologous genes, Cphy3030 gene; anaerobic cellulolytic bacterium
-
2.4.1.247 Lachnoclostridium phytofermentans A9KHK4 bacterium possesses three GalHexNAcP homologous genes, cphy1920 gene; anaerobic cellulolytic bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.211
-
 Lachnoclostridium phytofermentans
2.4.1.247
-
 Lachnoclostridium phytofermentans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.211 galacto-N-biose + phosphate
-
 Lachnoclostridium phytofermentans alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine
-
 r
2.4.1.211 lacto-N-biose I + phosphate
-
 Lachnoclostridium phytofermentans alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
 r
2.4.1.211 lacto-N-biose I + phosphate
-
 Lachnoclostridium phytofermentans alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine
-
 ?
2.4.1.211 additional information Cphy0577 protein does not show activity on either L-Rha, D-Glc, or D-Gal Lachnoclostridium phytofermentans ?
-
 ?
2.4.1.211 additional information Cphy3030 protein does not show activity on either L-Rha, D-Glc, or D-Gal Lachnoclostridium phytofermentans ?
-
 ?
2.4.1.247 2-deoxy-D-glucose + alpha-D-galactose 1-phosphate when D-glucose derivatives are used as acceptors, reaction products are beta-1,3-galactosides Lachnoclostridium phytofermentans D-galactosyl-beta-1,3-2-deoxy-D-glucose + phosphate
-
 r
2.4.1.247 alpha-D-galactose 1-phosphate + L-rhamnose highest activity Lachnoclostridium phytofermentans D-galactosyl-beta-1,4-L-rhamnose + phosphate
-
 r
2.4.1.247 D-galactose + alpha-D-galactose 1-phosphate
-
 Lachnoclostridium phytofermentans D-galactosyl-beta-1,3-D-galactose + phosphate
-
 r
2.4.1.247 D-glucose + alpha-D-galactose 1-phosphate when D-glucose derivatives are used as acceptors, reaction products are beta-1,3-galactosides Lachnoclostridium phytofermentans D-galactosyl-beta-1,3-D-glucose + phosphate
-
 r
2.4.1.247 L-lyxose + alpha-D-galactose 1-phosphate
-
 Lachnoclostridium phytofermentans beta-D-galactosyl-(1->4)-L-lyxose + phosphate
-
 r
2.4.1.247 L-mannose + alpha-D-galactose 1-phosphate
-
 Lachnoclostridium phytofermentans D-galactosyl-beta-1,4-L-mannose + phosphate
-
 r
2.4.1.247 additional information phosphorylyzes neither galacto-N-biose (D-Gal-beta1,3-D-GalNAc) nor lacto-N-biose I(D-Gal-beta1,3-D-GlcNAc), thus indicating that Cphy1920 protein is not GalHexNAcP Lachnoclostridium phytofermentans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.211 cphy0577 protein
-
 Lachnoclostridium phytofermentans
2.4.1.211 cphy3030 protein
-
 Lachnoclostridium phytofermentans
2.4.1.211 D-galactosyl-beta1,3-N-acetyl-D-hexosamine phosphorylase
-
 Lachnoclostridium phytofermentans
2.4.1.211 GalHexNAcP
-
 Lachnoclostridium phytofermentans
2.4.1.211 GNB/LNB phosphorylase
-
 Lachnoclostridium phytofermentans
2.4.1.247 cphy1920 protein
-
 Lachnoclostridium phytofermentans
2.4.1.247 D-galactosyl-beta-1,4-L-rhamnose phosphorylase
-
 Lachnoclostridium phytofermentans
2.4.1.247 GalRhaP
-
 Lachnoclostridium phytofermentans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.211 30
-
 protein is thermostable up to 30°C Lachnoclostridium phytofermentans
2.4.1.211 45
-
 protein is thermostable up to 45°C Lachnoclostridium phytofermentans
2.4.1.247 37
-
 protein is thermostable up to 37°C Lachnoclostridium phytofermentans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.211 34
-
 N-acetyl-D-galactosamine
-
 Lachnoclostridium phytofermentans
2.4.1.211 39
-
 lacto-N-biose I
-
 Lachnoclostridium phytofermentans
2.4.1.211 39
-
 galacto-N-biose
-
 Lachnoclostridium phytofermentans
2.4.1.211 40
-
 lacto-N-biose I
-
 Lachnoclostridium phytofermentans
2.4.1.211 42
-
 N-acetyl-D-glucosamine
-
 Lachnoclostridium phytofermentans
2.4.1.211 48
-
 N-acetyl-D-galactosamine
-
 Lachnoclostridium phytofermentans
2.4.1.211 49
-
 N-acetyl-D-glucosamine
-
 Lachnoclostridium phytofermentans
2.4.1.211 50
-
 galacto-N-biose
-
 Lachnoclostridium phytofermentans
2.4.1.247 2 8 2-deoxy-D-glucose
-
 Lachnoclostridium phytofermentans
2.4.1.247 24
-
 D-galactose
-
 Lachnoclostridium phytofermentans
2.4.1.247 45
-
 D-galactosyl-beta-1,4-L-rhamnose
-
 Lachnoclostridium phytofermentans
2.4.1.247 74
-
 L-rhamnose
-
 Lachnoclostridium phytofermentans
2.4.1.247 85
-
 D-glucose
-
 Lachnoclostridium phytofermentans
2.4.1.247 120
-
 L-Lyxose
-
 Lachnoclostridium phytofermentans
2.4.1.247 170
-
 L-Mannose
-
 Lachnoclostridium phytofermentans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.211 6 7 optimum activity at neutral pH Lachnoclostridium phytofermentans
2.4.1.247 6 7 optimum activity at neutral pH Lachnoclostridium phytofermentans