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Literature summary extracted from

  • Hoeberichts, F.A.; Vaeck, E.; Kiddle, G.; Coppens, E.; van de Cotte, B.; Adamantidis, A.; Ormenese, S.; Foyer, C.H.; Zabeau, M.; Inze, D.; Perilleux, C.; Van Breusegem, F.; Vuylsteke, M.
    A temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death (2008), J. Biol. Chem., 283, 5708-5718.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.8 expressed in Escherichia coli strain BL21(DE3)pLysE cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
5.4.2.8 G7R mutant exhibits activity similar to wild type enzyme Arabidopsis thaliana
5.4.2.8 G7R/R37Q temperature-sensitive Arabidopsis thaliana PMM-12 mutant (G7R/R37Q) has lower PMM protein and enzyme activity levels than the wild type enzyme Arabidopsis thaliana
5.4.2.8 R37Q mutant exhibits decreased activity similar to wild type enzyme Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.8 0.068
-
 alpha-D-mannose 1-phosphate mutant enzyme G7R/R37Q, at 28°C Arabidopsis thaliana
5.4.2.8 0.111
-
 alpha-D-mannose 1-phosphate mutant enzyme R37Q, at 28°C Arabidopsis thaliana
5.4.2.8 0.2
-
 alpha-D-mannose 1-phosphate wild type enzyme, at 28°C Arabidopsis thaliana
5.4.2.8 0.204
-
 alpha-D-mannose 1-phosphate mutant enzyme G7R/R37Q, at 18°C Arabidopsis thaliana
5.4.2.8 0.229
-
 alpha-D-mannose 1-phosphate mutant enzyme G7R, at 28°C Arabidopsis thaliana
5.4.2.8 0.294
-
 alpha-D-mannose 1-phosphate mutant enzyme R37Q, at 18°C Arabidopsis thaliana
5.4.2.8 0.387
-
 alpha-D-mannose 1-phosphate wild type enzyme, at 18°C Arabidopsis thaliana
5.4.2.8 0.475
-
 alpha-D-mannose 1-phosphate mutant enzyme G7R, at 18°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.8 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.8 Talon metal affinity resin chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.2.8 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.8 alpha-D-mannose 1-phosphate
-
 Arabidopsis thaliana alpha-D-mannose 6-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.4.2.8 PMM
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.2.8 0.18
-
 alpha-D-mannose 1-phosphate mutant enzyme G7R/R37Q, at 18°C Arabidopsis thaliana
5.4.2.8 0.24
-
 alpha-D-mannose 1-phosphate mutant enzyme G7R/R37Q, at 28°C Arabidopsis thaliana
5.4.2.8 0.44
-
 alpha-D-mannose 1-phosphate mutant enzyme R37Q, at 18°C Arabidopsis thaliana
5.4.2.8 0.67
-
 alpha-D-mannose 1-phosphate mutant enzyme R37Q, at 28°C Arabidopsis thaliana
5.4.2.8 4.13
-
 alpha-D-mannose 1-phosphate wild type enzyme, at 18°C Arabidopsis thaliana
5.4.2.8 4.18
-
 alpha-D-mannose 1-phosphate mutant enzyme G7R, at 18°C Arabidopsis thaliana
5.4.2.8 10.01
-
 alpha-D-mannose 1-phosphate wild type enzyme, at 28°C Arabidopsis thaliana
5.4.2.8 10.31
-
 alpha-D-mannose 1-phosphate mutant enzyme G7R, at 28°C Arabidopsis thaliana