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Literature summary extracted from

  • Werther, T.; Spinka, M.; Tittmann, K.; Schütz, A.; Golbik, R.; Mrestani-Klaus, C.; Hübner, G.; König, S.
    Amino acids allosterically regulate the thiamine diphosphate-dependent alpha-keto acid decarboxylase from Mycobacterium tuberculosis (2008), J. Biol. Chem., 283, 5344-5354.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.72 additional information stopped-flow kinetics show that MtKDC is allosterically activated by 2-oxoacids, also amino acids are potent activators Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.72 expressed in Escherichia coli Rosetta 2 (DE3) and various Escherichia coli strains Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.72 pyruvate weak substrate inhibition Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.72 0.09
-
(indol-3-yl)pyruvate
-
Mycobacterium tuberculosis
4.1.1.72 0.4
-
pyruvate
-
Mycobacterium tuberculosis
4.1.1.72 0.83
-
2-Oxohexanoate
-
Mycobacterium tuberculosis
4.1.1.72 1.17
-
phenylpyruvate
-
Mycobacterium tuberculosis
4.1.1.72 1.21
-
2-oxopentanoate
-
Mycobacterium tuberculosis
4.1.1.72 20.25
-
2-oxo-3-methylpentanoate
-
Mycobacterium tuberculosis
4.1.1.72 26.11
-
benzoylformate
-
Mycobacterium tuberculosis
4.1.1.72 138.3
-
2-oxobutyrate
-
Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.72 59780
-
calculated from the deduced amino acid sequence Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.72 Mycobacterium tuberculosis P9WG37
-
-
4.1.1.72 Mycobacterium tuberculosis H37Rv P9WG37
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.72 anion exchange chromatography and gel filtration Mycobacterium tuberculosis

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.1.1.72
-
Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.72 (indol-3-yl)pyruvate
-
Mycobacterium tuberculosis (indol-3-yl)acetaldehyde + CO2
-
?
4.1.1.72 (indol-3-yl)pyruvate
-
Mycobacterium tuberculosis H37Rv (indol-3-yl)acetaldehyde + CO2
-
?
4.1.1.72 2-oxo-3-methylpentanoate
-
Mycobacterium tuberculosis 2-methylbutanal + CO2
-
?
4.1.1.72 2-oxobutyrate
-
Mycobacterium tuberculosis propionaldehyde + CO2
-
?
4.1.1.72 2-oxohexanoate
-
Mycobacterium tuberculosis n-pentanal + CO2
-
?
4.1.1.72 2-oxoisohexanoate
-
Mycobacterium tuberculosis ? + CO2
-
?
4.1.1.72 2-oxoisopentanoate
-
Mycobacterium tuberculosis 2-methylpropanal + CO2
-
?
4.1.1.72 2-oxopentanoate
-
Mycobacterium tuberculosis n-butanal + CO2
-
?
4.1.1.72 4-hydroxyphenylpyruvate
-
Mycobacterium tuberculosis 4-hydroxyphenylacetaldehyde + CO2
-
?
4.1.1.72 4-hydroxyphenylpyruvate
-
Mycobacterium tuberculosis H37Rv 4-hydroxyphenylacetaldehyde + CO2
-
?
4.1.1.72 benzoylformate
-
Mycobacterium tuberculosis ? + CO2
-
?
4.1.1.72 additional information enzyme is found to convert a broad spectrum of branched-chain and aromatic alpha-keto acids. Determined the catalytic constants for the conversion of 11 aliphatic, aromatic, and branched-chain alpha-keto acids Mycobacterium tuberculosis ?
-
?
4.1.1.72 additional information enzyme is found to convert a broad spectrum of branched-chain and aromatic alpha-keto acids. Determined the catalytic constants for the conversion of 11 aliphatic, aromatic, and branched-chain alpha-keto acids Mycobacterium tuberculosis H37Rv ?
-
?
4.1.1.72 Phenylpyruvate
-
Mycobacterium tuberculosis Phenylacetaldehyde + CO2
-
?
4.1.1.72 Phenylpyruvate
-
Mycobacterium tuberculosis H37Rv Phenylacetaldehyde + CO2
-
?
4.1.1.72 pyruvate
-
Mycobacterium tuberculosis acetaldehyde + CO2
-
?
4.1.1.72 pyruvate
-
Mycobacterium tuberculosis H37Rv acetaldehyde + CO2
-
?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.72 alpha-keto acid decarboxylase
-
Mycobacterium tuberculosis
4.1.1.72 MtKDC
-
Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.72 2.14
-
pyruvate
-
Mycobacterium tuberculosis
4.1.1.72 2.98
-
(indol-3-yl)pyruvate
-
Mycobacterium tuberculosis
4.1.1.72 3.89
-
4-hydroxyphenylpyruvate
-
Mycobacterium tuberculosis
4.1.1.72 5.98
-
2-oxobutyrate
-
Mycobacterium tuberculosis
4.1.1.72 6.95
-
2-oxopentanoate
-
Mycobacterium tuberculosis
4.1.1.72 7.06
-
2-Oxohexanoate
-
Mycobacterium tuberculosis
4.1.1.72 7.5
-
phenylpyruvate
-
Mycobacterium tuberculosis
4.1.1.72 18.57
-
benzoylformate
-
Mycobacterium tuberculosis
4.1.1.72 19.75
-
2-oxo-3-methylpentanoate
-
Mycobacterium tuberculosis
4.1.1.72 28.35
-
2-oxoisopentanoate
-
Mycobacterium tuberculosis
4.1.1.72 31.5
-
2-Oxoisohexanoate
-
Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.72 6.5
-
assay at Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.72 thiamine diphosphate
-
Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.72 124
-
pyruvate weak substrate inhibition is detected for pyruvate Mycobacterium tuberculosis