EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.72 | additional information | stopped-flow kinetics show that MtKDC is allosterically activated by 2-oxoacids, also amino acids are potent activators | Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.72 | expressed in Escherichia coli Rosetta 2 (DE3) and various Escherichia coli strains | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.72 | pyruvate | weak substrate inhibition | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.72 | 0.09 | - |
(indol-3-yl)pyruvate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 0.4 | - |
pyruvate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 0.83 | - |
2-Oxohexanoate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 1.17 | - |
phenylpyruvate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 1.21 | - |
2-oxopentanoate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 20.25 | - |
2-oxo-3-methylpentanoate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 26.11 | - |
benzoylformate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 138.3 | - |
2-oxobutyrate | - |
Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.72 | 59780 | - |
calculated from the deduced amino acid sequence | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.72 | Mycobacterium tuberculosis | P9WG37 | - |
- |
4.1.1.72 | Mycobacterium tuberculosis H37Rv | P9WG37 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.72 | anion exchange chromatography and gel filtration | Mycobacterium tuberculosis |
EC Number | Renatured (Comment) | Organism |
---|---|---|
4.1.1.72 | - |
Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.72 | (indol-3-yl)pyruvate | - |
Mycobacterium tuberculosis | (indol-3-yl)acetaldehyde + CO2 | - |
? | |
4.1.1.72 | (indol-3-yl)pyruvate | - |
Mycobacterium tuberculosis H37Rv | (indol-3-yl)acetaldehyde + CO2 | - |
? | |
4.1.1.72 | 2-oxo-3-methylpentanoate | - |
Mycobacterium tuberculosis | 2-methylbutanal + CO2 | - |
? | |
4.1.1.72 | 2-oxobutyrate | - |
Mycobacterium tuberculosis | propionaldehyde + CO2 | - |
? | |
4.1.1.72 | 2-oxohexanoate | - |
Mycobacterium tuberculosis | n-pentanal + CO2 | - |
? | |
4.1.1.72 | 2-oxoisohexanoate | - |
Mycobacterium tuberculosis | ? + CO2 | - |
? | |
4.1.1.72 | 2-oxoisopentanoate | - |
Mycobacterium tuberculosis | 2-methylpropanal + CO2 | - |
? | |
4.1.1.72 | 2-oxopentanoate | - |
Mycobacterium tuberculosis | n-butanal + CO2 | - |
? | |
4.1.1.72 | 4-hydroxyphenylpyruvate | - |
Mycobacterium tuberculosis | 4-hydroxyphenylacetaldehyde + CO2 | - |
? | |
4.1.1.72 | 4-hydroxyphenylpyruvate | - |
Mycobacterium tuberculosis H37Rv | 4-hydroxyphenylacetaldehyde + CO2 | - |
? | |
4.1.1.72 | benzoylformate | - |
Mycobacterium tuberculosis | ? + CO2 | - |
? | |
4.1.1.72 | additional information | enzyme is found to convert a broad spectrum of branched-chain and aromatic alpha-keto acids. Determined the catalytic constants for the conversion of 11 aliphatic, aromatic, and branched-chain alpha-keto acids | Mycobacterium tuberculosis | ? | - |
? | |
4.1.1.72 | additional information | enzyme is found to convert a broad spectrum of branched-chain and aromatic alpha-keto acids. Determined the catalytic constants for the conversion of 11 aliphatic, aromatic, and branched-chain alpha-keto acids | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
4.1.1.72 | Phenylpyruvate | - |
Mycobacterium tuberculosis | Phenylacetaldehyde + CO2 | - |
? | |
4.1.1.72 | Phenylpyruvate | - |
Mycobacterium tuberculosis H37Rv | Phenylacetaldehyde + CO2 | - |
? | |
4.1.1.72 | pyruvate | - |
Mycobacterium tuberculosis | acetaldehyde + CO2 | - |
? | |
4.1.1.72 | pyruvate | - |
Mycobacterium tuberculosis H37Rv | acetaldehyde + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.72 | alpha-keto acid decarboxylase | - |
Mycobacterium tuberculosis |
4.1.1.72 | MtKDC | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.72 | 2.14 | - |
pyruvate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 2.98 | - |
(indol-3-yl)pyruvate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 3.89 | - |
4-hydroxyphenylpyruvate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 5.98 | - |
2-oxobutyrate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 6.95 | - |
2-oxopentanoate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 7.06 | - |
2-Oxohexanoate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 7.5 | - |
phenylpyruvate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 18.57 | - |
benzoylformate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 19.75 | - |
2-oxo-3-methylpentanoate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 28.35 | - |
2-oxoisopentanoate | - |
Mycobacterium tuberculosis | |
4.1.1.72 | 31.5 | - |
2-Oxoisohexanoate | - |
Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.72 | 6.5 | - |
assay at | Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.72 | thiamine diphosphate | - |
Mycobacterium tuberculosis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.72 | 124 | - |
pyruvate | weak substrate inhibition is detected for pyruvate | Mycobacterium tuberculosis |