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Literature summary extracted from

  • Sharma, A.K.; Zhou, G.P.; Kupferman, J.; Surks, H.K.; Christensen, E.N.; Chou, J.J.; Mendelsohn, M.E.; Rigby, A.C.
    Probing the interaction between the coiled-coil leucine zipper of cGMP-dependent protein kinase Ialpha and the C-terminus of the myosin binding subunit of the myosin light chain phosphatase (2008), J. Biol. Chem., 283, 32860-32869.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.12 expressed in Escherichia coli, with glutathione-S-transferase-fusion tag Homo sapiens
3.1.3.53 expressed in Escherichia coli XL-10 Gold cells Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.53 130000
-
 myosin binding subunit, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.12 Homo sapiens
-
-
-
3.1.3.53 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.12 Ni2+-NTA agarose column chromatography and Superdex 75 gel filtration Homo sapiens
3.1.3.53 Ni2+-NTA-agarose column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.12 ATP + myosin phosphatase
-
 Homo sapiens ADP + phosphorylated RhoA
-
 ?
3.1.3.53 myosin light-chain phosphate + H2O
-
 Homo sapiens myosin light-chain + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.12 cGMP-dependent protein kinase Ialpha
-
 Homo sapiens
2.7.11.12 PKG-Ialpha
-
 Homo sapiens
3.1.3.53 MBS myosin binding subunit of myosin light chain phosphatase Homo sapiens
3.1.3.53 MLCP
-
 Homo sapiens
3.1.3.53 myosin phosphatase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.12 ATP
-
 Homo sapiens
2.7.11.12 cGMP dependent Homo sapiens