Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ishikawa, T.; Nishikawa, H.; Gao, Y.; Sawa, Y.; Shibata, H.; Yabuta, Y.; Maruta, T.; Shigeoka, S.
    The pathway via D-galacturonate/L-galactonate is significant for ascorbate biosynthesis in Euglena gracilis: identification and functional characterization of aldonolactonase (2008), J. Biol. Chem., 283, 31133-31141.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.17 hydrogen peroxide stimulates activity at 0.1-0.5 mM Euglena gracilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.17 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.17 EDTA strong inhibition Euglena gracilis
3.1.1.17 additional information not inhibited by L-ascorbic acid and dithiothreitol Euglena gracilis
3.1.1.17 N-ethylmaleimide
-
 Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.17 1.55
-
 L-galactonic acid recombinant enzyme Euglena gracilis
3.1.1.17 1.67
-
 L-galactono-1,4-lactone recombinant enzyme Euglena gracilis
3.1.1.17 2.08
-
 D-gulono-1,5-lactone recombinant enzyme Euglena gracilis
3.1.1.17 3.05
-
 L-gulono-1,4-lactone recombinant enzyme Euglena gracilis
3.1.1.17 4.55
-
 L-Gulonic acid recombinant enzyme Euglena gracilis
3.1.1.17 9.4
-
 D-glucono-1,5-lactone wild type enzyme Euglena gracilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.17 additional information Mn2+, Co2+, Mg2+, and Ca2+ have a significant low effect on the activity to 19, 9, 8, and 7%, respectively Euglena gracilis
3.1.1.17 Zn2+ the enzyme requires a divalent metal ion for maximum activity which is obtained by the addition of 0.075 mM Zn2+ Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.17 34000
-
 about 34000 Da, recombinant enzyme, SDS-PAGE Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.17 Euglena gracilis A9CPS8
-
-
3.1.1.17 Euglena gracilis Z A9CPS8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.17 TALON metal affinity resin column chromatography Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.17 0.8
-
 wild type enzyme, using L-galactono-1,4-lactone as substrate Euglena gracilis
3.1.1.17 2.7
-
 wild type enzyme, using L-gulono-1,4-lactone as substrate Euglena gracilis
3.1.1.17 5.4
-
 wild type enzyme, using L-glucono-1,5-lactone as substrate Euglena gracilis
3.1.1.17 9.4
-
 wild type enzyme, using D-galactono-1,4-lactone as substrate Euglena gracilis
3.1.1.17 12
-
 wild type enzyme, using D-gulono-1,4-lactone as substrate Euglena gracilis
3.1.1.17 20.1
-
 recombinant enzyme, using D-gulono-1,4-lactone as substrate Euglena gracilis
3.1.1.17 24.5
-
 recombinant enzyme, using L-galactono-1,4-lactone as substrate Euglena gracilis
3.1.1.17 27.3
-
 recombinant enzyme, using D-galactono-1,4-lactone as substrate Euglena gracilis
3.1.1.17 39
-
 recombinant enzyme, using D-glucono-1,5-lactone as substrate Euglena gracilis
3.1.1.17 40.9
-
 recombinant enzyme, using L-gulono-1,4-lactone as substrate Euglena gracilis
3.1.1.17 217
-
 wild type enzyme, using D-glucono-1,5-lactone as substrate Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.17 D-galactonic acid + H2O
-
 Euglena gracilis ?
-
 ?
3.1.1.17 D-galactonic acid + H2O
-
 Euglena gracilis Z ?
-
 ?
3.1.1.17 D-galactono-1,4-lactone + H2O wild type enzyme shows 4.33% specific activity, recombinant enzyme shows 70% specific activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis ?
-
 r
3.1.1.17 D-galactono-1,4-lactone + H2O wild type enzyme shows 4.33% specific activity, recombinant enzyme shows 70% specific activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis Z ?
-
 r
3.1.1.17 D-glucono-1,5-lactone + H2O wild type and recombinant enzyme show 100% specific activity Euglena gracilis D-gluconate
-
 r
3.1.1.17 D-glucono-1,5-lactone + H2O wild type and recombinant enzyme show 100% specific activity Euglena gracilis Z D-gluconate
-
 r
3.1.1.17 D-gulono-1,4-lactone + H2O wild type enzyme shows 12.0% specific activity, recombinant enzyme shows 20.1% specific activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis D-galactonate
-
 r
3.1.1.17 D-gulono-1,4-lactone + H2O wild type enzyme shows 12.0% specific activity, recombinant enzyme shows 20.1% specific activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis Z D-galactonate
-
 r
3.1.1.17 D-gulono-1,5-lactone + H2O
-
 Euglena gracilis ?
-
 ?
3.1.1.17 L-galactonic acid + H2O
-
 Euglena gracilis ?
-
 ?
3.1.1.17 L-galactono-1,4-lactone + H2O wild type enzyme shows 0.37% specific activity, recombinant enzyme shows 62.8% specific activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis ?
-
 r
3.1.1.17 L-glucono-1,5-lactone + H2O wild type enzyme shows 2.49% specific activity, recombinant enzyme shows no activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis L-gluconate
-
 r
3.1.1.17 L-glucono-1,5-lactone + H2O wild type enzyme shows 2.49% specific activity, recombinant enzyme shows no activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis Z L-gluconate
-
 r
3.1.1.17 L-gulonic acid + H2O
-
 Euglena gracilis ?
-
 ?
3.1.1.17 L-gulono-1,4-lactone + H2O wild type enzyme shows 1.24% specific activity, recombinant enzyme shows 105% specific activity compared to the reaction with D-glucono-1,5-lactone Euglena gracilis L-gulonate
-
 r

Synonyms

EC Number Synonyms Comment Organism
3.1.1.17 ALase
-
 Euglena gracilis
3.1.1.17 aldonolactonase
-
 Euglena gracilis
3.1.1.17 SMP30
-
 Euglena gracilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.17 160
-
 D-gulono-1,5-lactone recombinant enzyme Euglena gracilis
3.1.1.17 192
-
 D-glucono-1,5-lactone wild type enzyme Euglena gracilis
3.1.1.17 450
-
 L-galactono-1,4-lactone recombinant enzyme Euglena gracilis
3.1.1.17 615
-
 L-galactonic acid recombinant enzyme Euglena gracilis
3.1.1.17 1040
-
 L-gulono-1,4-lactone recombinant enzyme Euglena gracilis
3.1.1.17 1503
-
 L-Gulonic acid recombinant enzyme Euglena gracilis