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Literature summary extracted from

  • Sandoval, F.J.; Zhang, Y.; Roje, S.
    Flavin nucleotide metabolism in plants: monofunctional enzymes synthesize FAD in plastids (2008), J. Biol. Chem., 283, 30890-30900.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.2 cloned in Escherichia coli Arabidopsis thaliana
2.7.7.2 expression in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.2 additional information recombinant protein has FAD synthetase activity, but not riboflavin kinase activity Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.2 ATP substrate inhibition; substrate inhibition; substrate inhibition Arabidopsis thaliana
2.7.7.2 FMN substrate inhibition; substrate inhibition; substrate inhibition Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.2 0.0111
-
 ATP pH 8.5 Arabidopsis thaliana
2.7.7.2 0.0111
-
 ATP at pH 8.5 Arabidopsis thaliana
2.7.7.2 0.0131
-
 ATP pH 8.5 Arabidopsis thaliana
2.7.7.2 0.0131
-
 ATP at pH 8.5 Arabidopsis thaliana
2.7.7.2 0.0189
-
 FMN pH 8.5 Arabidopsis thaliana
2.7.7.2 0.0189
-
 FMN at pH 8.5 Arabidopsis thaliana
2.7.7.2 0.0208
-
 FMN pH 8.5 Arabidopsis thaliana
2.7.7.2 0.0208
-
 FMN at pH 8.5 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.2 chloroplast
-
 Arabidopsis thaliana 9507
-
2.7.7.2 plastid
-
 Arabidopsis thaliana 9536
-
2.7.7.2 protoplast
-
 Arabidopsis thaliana
-
-
3.6.1.18 chloroplast
-
 Pisum sativum 9507
-
3.6.1.18 mitochondrion
-
 Pisum sativum 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.18 Mg2+ activity is Mg2+-dependent Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.2 28800
-
 gel filtration Arabidopsis thaliana
2.7.7.2 28800
-
 estimated by gel filtration Arabidopsis thaliana
2.7.7.2 29200
-
 gel filtration Arabidopsis thaliana
2.7.7.2 29200
-
 estimated by gel filtration Arabidopsis thaliana
2.7.7.2 33900
-
 1 * 33900, SDS-PAGE and calculated Arabidopsis thaliana
2.7.7.2 34200
-
 1 * 34200, SDS-PAGE and calculated Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.2 ATP + FMN Arabidopsis thaliana
-
 diphosphate + FAD
-
 ?
3.6.1.18 FAD + H2O Pisum sativum
-
 AMP + FMN
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.2 Arabidopsis thaliana Q0WS47 isoform RibF1
-
2.7.7.2 Arabidopsis thaliana Q8VZR0
-
-
2.7.7.2 Arabidopsis thaliana Q9FMW8
-
-
2.7.7.2 Arabidopsis thaliana Q9FMW8 This UniProt-ID has been deleted; isoform RibF1
-
3.6.1.18 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.2 by ion exchange chromatography and gel filtration Arabidopsis thaliana
2.7.7.2 by Ni-NTA affinity chromatography, ion exchange chromatography and gel filtration Arabidopsis thaliana
2.7.7.2 recombinant protein Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.2 bolting stage
-
 Arabidopsis thaliana
-
2.7.7.2 flower
-
 Arabidopsis thaliana
-
2.7.7.2 additional information relative expression levels show little or no variation among the organs and at the developmental stages examined. Exceptions are mature siliques and germinated seeds, which expresse 50-100% more AtRibF1 mRNA than AtRibF2 mRNA Arabidopsis thaliana
-
2.7.7.2 additional information expression levels of AtRibF1 show little or no variation among the organs and at the developmental stages examined, 2 exceptions are mature siliques and germinated seeds, which express 50-100% more AtRibF1 mRNA, AtRibF1 is a housekeeping enzyme needed by all plant organs throughout development Arabidopsis thaliana
-
2.7.7.2 additional information expression levels of AtRibF2 show little or no variation among the organs and at the developmental stages examined, AtRibF2 are housekeeping enzyme needed by all plant organs throughout development Arabidopsis thaliana
-
2.7.7.2 rosette
-
 Arabidopsis thaliana
-
2.7.7.2 seed
-
 Arabidopsis thaliana
-
2.7.7.2 seed mature siliques and germinated seeds expresse 50-100% more AtRibF1 mRNA than AtRibF2 mRNA Arabidopsis thaliana
-
2.7.7.2 seedling
-
 Arabidopsis thaliana
-
2.7.7.2 silique
-
 Arabidopsis thaliana
-
2.7.7.2 silique mature siliques and germinated seeds expresse 50-100% more AtRibF1 mRNA than AtRibF2 mRNA Arabidopsis thaliana
-
3.6.1.18 leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.18 0.00003
-
 at pH 7.5 in chloroplasts, in 0.1 M Tris-HCl, 15 mM MgCl2, 1 mM tris(hydroxypropyl)phosphine and 0.05 mM flavin Pisum sativum
3.6.1.18 0.0000384
-
 at pH 8.5 in chloroplasts, in 0.1 M Tris-HCl, 15 mM MgCl2, 1 mM tris(hydroxypropyl)phosphine and 0.05 mM flavin Pisum sativum
3.6.1.18 0.000343
-
 at pH 7.5 in mitochondria, in 0.1 M Tris-HCl, 15 mM MgCl2, 1 mM tris(hydroxypropyl)phosphine and 0.05 mM flavin Pisum sativum
3.6.1.18 0.000482
-
 at pH 8.5 in mitochondria, in 0.1 M Tris-HCl, 15 mM MgCl2, 1 mM tris(hydroxypropyl)phosphine and 0.05 mM flavin Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.2 ATP + FMN
-
 Arabidopsis thaliana diphosphate + FAD
-
 ?
3.6.1.18 FAD + H2O
-
 Pisum sativum AMP + FMN
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.2 monomer 1 * 33900, SDS-PAGE and calculated Arabidopsis thaliana
2.7.7.2 monomer 1 * 34200, SDS-PAGE and calculated Arabidopsis thaliana
2.7.7.2 monomer estimated by gel filtration Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.7.7.2 AtRibF1
-
 Arabidopsis thaliana
2.7.7.2 AtRibF2
-
 Arabidopsis thaliana
2.7.7.2 FAD synthetase
-
 Arabidopsis thaliana
3.6.1.18 FAD diphosphatase
-
 Pisum sativum
3.6.1.18 FAD pyrophosphatase
-
 Pisum sativum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.2 0.0107
-
 ATP recombinant enzyme purified under native conditions, at pH 8.5 Arabidopsis thaliana
2.7.7.2 0.0108
-
 FMN recombinant enzyme purified under native conditions, at pH 8.5 Arabidopsis thaliana
2.7.7.2 0.011
-
 ATP pH 8.5 Arabidopsis thaliana
2.7.7.2 0.011
-
 FMN pH 8.5 Arabidopsis thaliana
2.7.7.2 0.0128
-
 ATP recombinant enzyme purified under native conditions, at pH 8.5 Arabidopsis thaliana
2.7.7.2 0.0128
-
 FMN recombinant enzyme purified under native conditions, at pH 8.5 Arabidopsis thaliana
2.7.7.2 0.013
-
 ATP pH 8.5 Arabidopsis thaliana
2.7.7.2 0.013
-
 FMN pH 8.5 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.7.2 FMN
-
 Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.2 0.00085
-
 FMN pH 8.5 Arabidopsis thaliana
2.7.7.2 0.00085
-
 FMN recombinant enzyme purified under native conditions, at pH 8.5 Arabidopsis thaliana
2.7.7.2 0.00092
-
 FMN pH 8.5 Arabidopsis thaliana
2.7.7.2 0.00092
-
 FMN recombinant enzyme purified under native conditions, at pH 8.5 Arabidopsis thaliana
2.7.7.2 0.013
-
 ATP pH 8.5 Arabidopsis thaliana
2.7.7.2 0.01302
-
 ATP recombinant enzyme purified under native conditions, at pH 8.5 Arabidopsis thaliana
2.7.7.2 0.01868
-
 ATP recombinant enzyme purified under native conditions, at pH 8.5 Arabidopsis thaliana
2.7.7.2 0.0187
-
 ATP pH 8.5 Arabidopsis thaliana