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Literature summary extracted from

  • Maruta, T.; Yonemitsu, M.; Yabuta, Y.; Tamoi, M.; Ishikawa, T.; Shigeoka, S.
    Arabidopsis phosphomannose isomerase 1, but not phosphomannose isomerase 2, is essential for ascorbic acid biosynthesis (2008), J. Biol. Chem., 283, 28842-28851.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.8 expression in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.8 additional information knockout of isoform Pmi2 does not affect the total ascorbic acid levels in leaves Arabidopsis thaliana
5.3.1.8 additional information reduction of Pmi1 expression by RNA interference results in substantial decrease in the total ascorbic acid content of leaves Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.8 Cd2+ 1 mM, no residual activity Arabidopsis thaliana
5.3.1.8 dithiothreitol 1 mM, 73% residual activity; 1 mM, 84% residual activity Arabidopsis thaliana
5.3.1.8 EDTA 2.5 mM, 54% residual activity; 2.5 mM, 86% residual activity Arabidopsis thaliana
5.3.1.8 L-ascorbic acid 1 mM, 77% residual activity Arabidopsis thaliana
5.3.1.8 p-chloromercuribenzoate 0.05 mM, 33% residual activity; 0.05 mM, 39% residual activity Arabidopsis thaliana
5.3.1.8 Zn2+ 1 mM, no residual activity Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.8 0.0413
-
 D-mannose 6-phosphate pH 7.5, 25°C Arabidopsis thaliana
5.3.1.8 0.372
-
 D-mannose 6-phosphate pH 7.5, 25°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.8 Arabidopsis thaliana
-
 isoform Pmi1
-
5.3.1.8 Arabidopsis thaliana
-
 isoform Pmi2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.3.1.8 leaf induction of expression under continuous light. Diurnal expression pattern in parallel with the total Pmi1 activity and the total ascorbic acid content in leaf Arabidopsis thaliana
-
5.3.1.8 additional information constitutive expression in both vegetative and reproductive organs Arabidopsis thaliana
-
5.3.1.8 additional information no expression in any organ under light. Under long term darkness, expression of isoform Pmi2 with concomitant decrease in ascorbic acid levels Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.8 D-Mannose 6-phosphate
-
 Arabidopsis thaliana D-Fructose 6-phosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.8 52
-
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.8 7.5
-
-
 Arabidopsis thaliana