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Literature summary extracted from

  • Duncan, R.E.; Sarkadi-Nagy, E.; Jaworski, K.; Ahmadian, M.; Sul, H.S.
    Identification and functional characterization of adipose-specific phospholipase A2 (AdPLA) (2008), J. Biol. Chem., 283, 25428-25436.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.4 overexpression of His-tagged and/or fluoresence-tagged phospholipase A2 in COS-7 cells, perinuclear region, membranes and cytosol, or 3T3-L1 preadipocytic cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 7,7-dimethyl-5,8-eicosadienoic acid
-
 Rattus norvegicus
3.1.1.4 arachidonyl trifluoromethyl ketone i.e. AACOCF3 Rattus norvegicus
3.1.1.4 methyl arachidonyl fluorophosphate i.e. MAFP, an inhibitor of cysteine-dependent phospholipases Rattus norvegicus
3.1.1.4 additional information no inhibition of AdPLA and sPLA2 by bromoenolactone Rattus norvegicus
3.1.1.4 thioetheramide
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.4 0.005
-
 1-palmitoyl-2-linoleoyl-phosphatidylethanolamine pH 8.0, 25°C, recombinant enzyme Rattus norvegicus
3.1.1.4 0.0073
-
 1-palmitoyl-2-linoleoyl-phosphatidylserine pH 8.0, 25°C, recombinant enzyme Rattus norvegicus
3.1.1.4 0.0082
-
 1-palmitoyl-2-arachidonyl-phosphatidylcholine pH 8.0, 25°C, recombinant enzyme Rattus norvegicus
3.1.1.4 0.0116
-
 1-palmitoyl-2-linoleoyl-phosphatidic acid pH 8.0, 25°C, recombinant enzyme Rattus norvegicus
3.1.1.4 0.0175
-
 1,2-dilinoleoyl-phosphatidylcholine pH 8.0, 25°C, recombinant enzyme Rattus norvegicus
3.1.1.4 0.0186
-
 1-palmitoyl-2-linoleoyl-phosphatidylcholine pH 8.0, 25°C, recombinant enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.4 cytosol AdPLA Rattus norvegicus 5829
-
3.1.1.4 membrane the adipose-specific phospholipase A2 AdPLA is membrane-associated Rattus norvegicus 16020
-
3.1.1.4 additional information subcellular localization of AdPLA, overview Rattus norvegicus
-
-
3.1.1.4 perinuclear space AdPLA Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.4 Ca2+ the adipose-specific phospholipase A2 is activated by Ca2+, but it is not essential for activity Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 additional information Rattus norvegicus phospholipases A2 catalyze hydrolysis of fatty acids from the sn-2 position of phospholipids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Rattus norvegicus
-
 female Zucker rats
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 colon
-
 Rattus norvegicus
-
3.1.1.4 gonad
-
 Rattus norvegicus
-
3.1.1.4 heart
-
 Rattus norvegicus
-
3.1.1.4 kidney
-
 Rattus norvegicus
-
3.1.1.4 liver
-
 Rattus norvegicus
-
3.1.1.4 lung
-
 Rattus norvegicus
-
3.1.1.4 additional information expression profiles of isozymes iPLA2beta, sPLA2alpha, and AdPLA, overview Rattus norvegicus
-
3.1.1.4 muscle
-
 Rattus norvegicus
-
3.1.1.4 skin
-
 Rattus norvegicus
-
3.1.1.4 spleen
-
 Rattus norvegicus
-
3.1.1.4 white adipose tissue AdPLA is highly expressed specifically in white adipose tissue and is induced during preadipocyte differentiation into adipocytes, overview Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 1,2-dilinoleoyl-phosphatidylcholine + H2O
-
 Rattus norvegicus 1-linoleoyl-sn-glycero-3-phosphocholine + linoleic acid
-
 ?
3.1.1.4 1,2-dilinoleoyl-phosphatidylcholine + H2O
-
 Rattus norvegicus 1-linoleoyl-glycerophosphorylcholine + linoleic acid
-
 ?
3.1.1.4 1-palmitoyl-2-arachidonyl-phosphatidylcholine + H2O
-
 Rattus norvegicus 1-palmitoyl-sn-glycero-3-phosphocholine + arachidonic acid
-
 ?
3.1.1.4 1-palmitoyl-2-linoleoyl-phosphatidic acid + H2O
-
 Rattus norvegicus 1-palmitoyl-sn-glycero-3-phosphate + linoleic acid
-
 ?
3.1.1.4 1-palmitoyl-2-linoleoyl-phosphatidylcholine + H2O
-
 Rattus norvegicus 1-palmitoyl-sn-glycero-3-phosphocholine + linoleic acid
-
 ?
3.1.1.4 1-palmitoyl-2-linoleoyl-phosphatidylethanolamine + H2O
-
 Rattus norvegicus 1-palmitoyl-sn-glycerophosphoethanolamine + linoleic acid
-
 ?
3.1.1.4 1-palmitoyl-2-linoleoyl-phosphatidylinositol + H2O
-
 Rattus norvegicus 1-palmitoyl-3-phosphoinositol + linoleic acid
-
 ?
3.1.1.4 1-palmitoyl-2-linoleoyl-phosphatidylserine + H2O
-
 Rattus norvegicus 1-palmitoyl-sn-glycero-3-phosphoserine + linoleic acid
-
 ?
3.1.1.4 hen egg phosphatidylcholine + H2O
-
 Rattus norvegicus ?
-
 ?
3.1.1.4 additional information phospholipases A2 catalyze hydrolysis of fatty acids from the sn-2 position of phospholipids Rattus norvegicus ?
-
 ?
3.1.1.4 additional information AdPLA is most likely a His-Cys PLA2, His23 and Cys113 are critical for activity of AdPLA, catalytic mechanism, overview. AdPLA has phospholipase activity but lacks acyltransferase activity Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.4 More AdPLA contains no characteristic serine lipase motif, i.e. GXSXG or GXSGS Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.4 AdPLA
-
 Rattus norvegicus
3.1.1.4 cPLA2alpha
-
 Rattus norvegicus
3.1.1.4 specific phospholipase A2
-
 Rattus norvegicus
3.1.1.4 sPLA2
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.4 25
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.4 8
-
 assay at Rattus norvegicus