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Literature summary extracted from
- Identifying key residues of sphinganine-1-phosphate lyase for function in vivo and in vitro (2008), J. Biol. Chem., 283, 20159-20169.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.2.27 | the plasmids pRS415, pRS416 and pESC are used | Saccharomyces cerevisiae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.1.2.27 | C344A | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | C65L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | C65L/K67L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | C65L/K67L/S70L/N71L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | DELTA1-16 | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | DELTA1-31 | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | DELTA1-31/C65L/K67L/S70L/N71L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | DELTA1-54 | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | DELTA1-57 | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | DELTA1-81 | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | H268A | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | H340A | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | K380A | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | K386A | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | K67L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | K67L/S70L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | N6A | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | N71L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | S70L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | S70L/N71L | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | Y554A | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
| 4.1.2.27 | Y554F | mutant constructed for structure-function analysis of Dpl1p to discover possible modes of regulation | Saccharomyces cerevisiae |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.1.2.27 | endoplasmic reticulum membrane | - |
Saccharomyces cerevisiae | 5789 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.27 | sphinganine 1-phosphate | Saccharomyces cerevisiae | - |
phosphoethanolamine + palmitaldehyde | - |
ir |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.2.27 | Saccharomyces cerevisiae | Q05567 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 4.1.2.27 | glycoprotein | - |
Saccharomyces cerevisiae |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.2.27 | FLAG-tagged purification is performed | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.27 | sphinganine 1-phosphate | - |
Saccharomyces cerevisiae | phosphoethanolamine + palmitaldehyde | - |
ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.2.27 | oligomer | - |
Saccharomyces cerevisiae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.2.27 | Dpl1p | - |
Saccharomyces cerevisiae |
| 4.1.2.27 | sphinganine-1-phosphate aldolase | - |
Saccharomyces cerevisiae |
| 4.1.2.27 | sphinganine-1-phosphate lyase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.27 | 30 | - |
activity assay | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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