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Literature summary extracted from

  • Creze, C.; Castang, S.; Derivery, E.; Haser, R.; Hugouvieux-Cotte-Pattat, N.; Shevchik, V.E.; Gouet, P.
    The crystal structure of pectate lyase peli from soft rot pathogen Erwinia chrysanthemi in complex with its substrate (2008), J. Biol. Chem., 283, 18260-18268.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.2 expressed in Escherichia coli Dickeya chrysanthemi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.2.2 crystal structure of PelI is solved to 1.45 A resolution. It consists of an N-terminal domain harboring a fibronectin type III fold linked to a catalytic domain displaying a parallel beta-helix topology. The N-terminal domain is located away from the active site and is not involved in the catalytic process. The structure of PelI in complex with its substrate, a tetragalacturonate, is solved to 2.3 A resolution. The sugar binds from subsites -2 to +2 in one monomer of the asymmetric unit, although it lies on subsites -1 to +3 in the other. These two Michaelis complexes are consistent with the dual mode of bond cleavage in this substrate. The bound sugar adopts a mixed 21 and 31 helical conformation similar to that reported in inactive mutants from families PL-1 and PL-10 Dickeya chrysanthemi

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.2 K224R mutant is completely defective in lyase activity Dickeya chrysanthemi
4.2.2.2 K249R mutant shows 40-60% of wild-type activity. At a higher Ca2+ concentration in the substrate medium, enzymatic activities of K249R and R252K mutants are less affected Dickeya chrysanthemi
4.2.2.2 R252K mutant shows 6-9% of wild-type activity. At a higher Ca2+ concentration in the substrate medium, enzymatic activities of K249R and R252K mutants are less affected Dickeya chrysanthemi

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Dickeya chrysanthemi O50325
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 pectin
-
 Dickeya chrysanthemi ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.2 pectate lyase
-
 Dickeya chrysanthemi
4.2.2.2 PelI
-
 Dickeya chrysanthemi
4.2.2.2 PL-3
-
 Dickeya chrysanthemi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 37
-
 assay at Dickeya chrysanthemi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 8.5
-
 assay at Dickeya chrysanthemi