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Literature summary extracted from

  • Kaneiwa, T.; Yamada, S.; Mizumoto, S.; Montano, A.M.; Mitani, S.; Sugahara, K.
    Identification of a novel chondroitin hydrolase in Caenorhabditis elegans (2008), J. Biol. Chem., 283, 14971-14979.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.35 3XFLAG tag Caenorhabditis elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.35 0.12
-
 chondroitin apparent KM, substrate calculated as disaccharide Caenorhabditis elegans
3.2.1.35 0.48
-
 chondroitin sulfate apparent KM, substrate calculated as disaccharide Caenorhabditis elegans
3.2.1.35 49.3
-
 hyaluronic acid from Streptococcus pyogenes (35 kDa) apparent KM, substrate calculated as disaccharide Caenorhabditis elegans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.35 80000
-
 SDS-PAGE, presumably glycosylated (53000 Da, expected molecular mass) Caenorhabditis elegans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Caenorhabditis elegans
-
 WormBase accession T22C8.2, cDNA: WBGene 00011923 (WormBase)
-
3.2.1.35 Caenorhabditis elegans N2
-
 WormBase accession T22C8.2, cDNA: WBGene 00011923 (WormBase)
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 chondroitin + H2O
-
 Caenorhabditis elegans GlcAbeta(1-3)GalNAcbeta(1-4)GlcAbeta(1-3)GalNAc + ? main product ir
3.2.1.35 chondroitin + H2O
-
 Caenorhabditis elegans N2 GlcAbeta(1-3)GalNAcbeta(1-4)GlcAbeta(1-3)GalNAc + ? main product ir
3.2.1.35 chondroitin sulfate + H2O
-
 Caenorhabditis elegans ?
-
 ?
3.2.1.35 chondroitin sulfate + H2O
-
 Caenorhabditis elegans N2 ?
-
 ?
3.2.1.35 hyaluronic acid + H2O
-
 Caenorhabditis elegans ?
-
 ?
3.2.1.35 hyaluronic acid + H2O
-
 Caenorhabditis elegans N2 ?
-
 ?
3.2.1.35 hyaluronic acid from Streptococcus pyogenes (35 kDa) + H2O
-
 Caenorhabditis elegans ?
-
 ?
3.2.1.35 hyaluronic acid from Streptococcus pyogenes (35 kDa) + H2O
-
 Caenorhabditis elegans N2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 hyaluronidase
-
 Caenorhabditis elegans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 6
-
-
 Caenorhabditis elegans