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Literature summary extracted from

  • Frese, M.A.; Schulz, S.; Dierks, T.
    Arylsulfatase G, a novel lysosomal sulfatase (2008), J. Biol. Chem., 283, 11388-11395.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.6.1 expressed in HT-1080 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.1 additional information ASG is not inhibited by warfarin even at concentrations close to the solubility limit Homo sapiens
3.1.6.1 phosphate acts as a strong, competitive ASG inhibitor, inhibition of ASG by phosphate is much stronger than by sulfate Homo sapiens
3.1.6.1 sulfate
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.6.1 lysosome
-
 Homo sapiens 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.1 63000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.6.1 additional information Homo sapiens ASG does not undergo lysosomal processing ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.1 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.6.1 glycoprotein ASG is a glycoprotein that binds specifically to mannose 6-phosphate receptors, the activity of arylsulfatase G depends on the Cys84 residue that is predicted to be posttranslationally converted to the critical active site Calpha-formylglycine Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.1 Ni-NTA column chromatography and HisTrap column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.1 kidney highest expression Homo sapiens
-
3.1.6.1 liver highest expression Homo sapiens
-
3.1.6.1 pancreas highest expression Homo sapiens
-
3.1.6.1 placenta
-
 Homo sapiens
-
3.1.6.1 skeletal muscle
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.1.6.1 4°C, pH 6.0, half-life of more than 100 days Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.1 4-methylumbelliferyl sulfate + H2O rather poor substrate Homo sapiens 4-methylumbelliferol + sulfate
-
 ?
3.1.6.1 4-nitrocatechol sulfate + H2O best substrate Homo sapiens 4-nitrocatechol + sulfate
-
 ?
3.1.6.1 4-nitrophenyl sulfate + H2O
-
 Homo sapiens 4-nitrophenol + sulfate
-
 ?
3.1.6.1 additional information ASG does not undergo lysosomal processing Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.6.1 monomer 1 * 63000, active enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.6.1 arylsulfatase G
-
 Homo sapiens
3.1.6.1 ASG
-
 Homo sapiens
3.1.6.1 KIAA1001
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.6.1 45 50 ASG cleaves 4-nitrocatechol sulfate most efficiently at 45-50°C Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.6.1 55
-
 ASG activity is retained even at relatively high temperatures above 55°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.1 5.4
-
 for the reaction with 4-nitrocatechol sulfate Homo sapiens
3.1.6.1 5.6
-
 for the reaction with 4-methylumbelliferyl sulfate Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.6.1 0.001
-
 at 10 mM 4-nitrocatechol sulfate Homo sapiens sulfate
3.1.6.1 0.05
-
 at 10 mM 4-nitrocatechol sulfate Homo sapiens phosphate