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Literature summary extracted from

  • Zhao, Y.; Zhang, J.; Li, H.; Li, Y.; Ren, J.; Luo, M.; Zheng, X.
    An NADPH sensor protein (HSCARG) down-regulates nitric oxide synthesis by association with argininosuccinate synthetase and is essential for epithelial cell viability (2008), J. Biol. Chem., 283, 11004-11013.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.5 expression alone or together with FLAG-tagged HSCARG or with HSCARG siRNA in HeLa and HEK-293 cells, expression analysis, overview Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.5 additional information enzyme overexpression induces a relocalization of HSCARG Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.5 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.5 ATP + L-citrulline + L-aspartate Homo sapiens rate-limiting enzyme in nitric oxide synthesis, negative feedback mechanism, overview AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 ?
6.3.4.5 additional information Homo sapiens the enzyme interacts and cooperates with NADPH sensor protein, HSCARG, in downregulation of nitric oxide synthesis, the enzyme interacts with the dimerization region of HSCARG, amino acid residues 153-189, HSCARG regulation of AS activity is crucial for maintaining the intracellular balance between redox state and nitric oxide levels, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.5 Homo sapiens P00966
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.5 epithelial cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.5 ATP + L-citrulline + L-aspartate
-
 Homo sapiens AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 ?
6.3.4.5 ATP + L-citrulline + L-aspartate rate-limiting enzyme in nitric oxide synthesis, negative feedback mechanism, overview Homo sapiens AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 ?
6.3.4.5 additional information the enzyme interacts and cooperates with NADPH sensor protein, HSCARG, in downregulation of nitric oxide synthesis, the enzyme interacts with the dimerization region of HSCARG, amino acid residues 153-189, HSCARG regulation of AS activity is crucial for maintaining the intracellular balance between redox state and nitric oxide levels, overview Homo sapiens ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.5 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.5 7.8
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.5 ATP
-
 Homo sapiens