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Literature summary extracted from

  • Cotner-Gohara, E.; Kim, I.K.; Tomkinson, A.E.; Ellenberger, T.
    Two DNA-binding and nick recognition modules in human DNA ligase III (2008), J. Biol. Chem., 283, 10764-10772.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.1 additional information deletion of the zinc finger does not significantly change LigIII DNA binding affinity, nor does it abrogate the specificity of the enzyme for a nicked substrate Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Na+ in 80 mM NaCl LigIII binds non-specifically to DNA, showing equal affinity for nicked and intact DNA, in 250 mM NaCl LigIII binds specifically to nicked DNA does not bind measurably to duplex DNA Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1 HiTrap nickel chelating column chromatography and Sephadex S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Homo sapiens AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 DNA ligase III
-
 Homo sapiens
6.5.1.1 LigIII
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.1 ATP
-
 Homo sapiens