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Literature summary extracted from

  • Hakansson, A.P.; Smith, A.W.
    Enzymatic characterization of dihydrolipoamide dehydrogenase from Streptococcus pneumoniae harboring its own substrate (2007), J. Biol. Chem., 282, 29521-29530.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.8.1.4 additional information the lipoyl protein domain (but not lipoic acid alone) plays a regulatory role in the enzymatic characteristics of pneumococcal DLDH Streptococcus pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.4 expressed in Escherichia coli M15 cells Streptococcus pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.4 K43A unable to express the mutant protein Streptococcus pneumoniae
1.8.1.4 K43R expresses well, but still has lipoic acid attached Streptococcus pneumoniae
1.8.1.4 additional information protein expressed without lipoic acid is indistinguishable from the wild-type protein. The protein without a lipoyl protein domain has a 2-3fold higher turnover number, a lower Ki for NADH, and a higher Ki for lipoamide compared with the other two enzymes Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.4 Lipoamide
-
 Streptococcus pneumoniae
1.8.1.4 NADH
-
 Streptococcus pneumoniae
1.8.1.4 potassium phosphate when purified DLDH is eluted directly into potassium phosphate buffer, the enzymatic activity rapidly decreases Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.4 0.04
-
 dihydrolipoamide recombinant DLDH expressed without without a lipoyl protein domain Streptococcus pneumoniae
1.8.1.4 0.056
-
 dihydrolipoamide recombinant DLDH Streptococcus pneumoniae
1.8.1.4 0.064
-
 dihydrolipoamide recombinant DLDH expressed without lipoic acid Streptococcus pneumoniae
1.8.1.4 0.82
-
 NAD+ recombinant DLDH expressed without without a lipoyl protein domain Streptococcus pneumoniae
1.8.1.4 0.985
-
 NAD+ recombinant DLDH expressed without lipoic acid Streptococcus pneumoniae
1.8.1.4 1.037
-
 NAD+ recombinant DLDH Streptococcus pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.4 61600
-
 full-length recombinant DLDH, sequence analysis Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4 gel filtration, at least 90% pure Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 dihydrolipoamide + NAD+
-
 Streptococcus pneumoniae lipoamide + NADH
-
 r
1.8.1.4 lipoamide + NADH
-
 Streptococcus pneumoniae dihydrolipoamide + NAD+
-
 r
1.8.1.4 nitro blue tetrazolium + NADH
-
 Streptococcus pneumoniae ? + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 dihydrolipoamide dehydrogenase
-
 Streptococcus pneumoniae
1.8.1.4 DLDH
-
 Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.4 160
-
 NAD+ recombinant DLDH Streptococcus pneumoniae
1.8.1.4 223
-
 NAD+ recombinant DLDH expressed without lipoic acid Streptococcus pneumoniae
1.8.1.4 506
-
 NAD+ recombinant DLDH expressed without without a lipoyl protein domain Streptococcus pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.4 8.3
-
 in the reverse reaction Streptococcus pneumoniae
1.8.1.4 8.5
-
 in the forward reaction Streptococcus pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 FAD
-
 Streptococcus pneumoniae
1.8.1.4 additional information unable to use NADP+ and NADPH Streptococcus pneumoniae
1.8.1.4 NAD+
-
 Streptococcus pneumoniae
1.8.1.4 NADH
-
 Streptococcus pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.4 0.0044
-
 NADH recombinant DLDH expressed without without a lipoyl protein domain, in the presence of 1.5 mM dihydrolipoamide Streptococcus pneumoniae
1.8.1.4 0.0107
-
 NADH recombinant DLDH expressed without lipoic acid, in the presence of 1.5 mM dihydrolipoamide Streptococcus pneumoniae
1.8.1.4 0.0149
-
 NADH recombinant DLDH, in the presence of 1.5 mM dihydrolipoamide Streptococcus pneumoniae
1.8.1.4 0.0351
-
 NADH recombinant DLDH expressed without without a lipoyl protein domain, in the presence of 2 mM NAD+ Streptococcus pneumoniae
1.8.1.4 0.0488
-
 Lipoamide recombinant DLDH expressed without lipoic acid, in the presence of 4 mM NAD+ Streptococcus pneumoniae
1.8.1.4 0.0566
-
 Lipoamide recombinant DLDH, in the presence of 4 mM NAD+ Streptococcus pneumoniae
1.8.1.4 0.0722
-
 NADH recombinant DLDH, in the presence of 2 mM NAD+ Streptococcus pneumoniae
1.8.1.4 0.084
-
 NADH recombinant DLDH expressed without lipoic acid, in the presence of 2 mM NAD+ Streptococcus pneumoniae
1.8.1.4 0.1899
-
 Lipoamide recombinant DLDH expressed without lipoic acid, in the presence of 1.5 mM dihydrolipoamide Streptococcus pneumoniae
1.8.1.4 0.1953
-
 Lipoamide recombinant DLDH, in the presence of 1.5 mM dihydrolipoamide Streptococcus pneumoniae
1.8.1.4 0.349
-
 Lipoamide recombinant DLDH expressed without without a lipoyl protein domain, in the presence of 4 mM NAD+ Streptococcus pneumoniae
1.8.1.4 0.8793
-
 Lipoamide recombinant DLDH expressed without without a lipoyl protein domain, in the presence of 1.5 mM dihydrolipoamide Streptococcus pneumoniae