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Literature summary extracted from

  • Shetterly, S.; Jost, F.; Watson, S.R.; Knegtel, R.; Macher, B.A.; Holmes, E.H.
    Site-specific fucosylation of sialylated polylactosamines by alpha1,3/4-fucosyltransferases-V and -VI is defined by amino acids near the N terminus of the catalytic domain (2007), J. Biol. Chem., 282, 24882-24892.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.65 expression of wild-type and mutant enzyme in COS-7 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.65 D125E site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity Homo sapiens
2.4.1.65 E111D site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity Homo sapiens
2.4.1.65 I126V site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity Homo sapiens
2.4.1.65 K73T site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
2.4.1.65 K73T/E111D site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
2.4.1.65 K73T/I75V/T87A insertion mutagenesis of FucT-VI, the mutant shows altered substrate specificity compared to the wild-type enzyme Homo sapiens
2.4.1.65 K73T/R110W site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
2.4.1.65 K73T/R110W/E111D/V112I site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
2.4.1.65 K97S site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity Homo sapiens
2.4.1.65 additional information construction of several chimeric mutants by domain swappig, overview Homo sapiens
2.4.1.65 R110W site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
2.4.1.65 R110W/E111D/V112I site-directed mutagenesis of FucT-VI, the mutant shows increased activity compared to the wild-type enzyme Homo sapiens
2.4.1.65 R96S site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity Homo sapiens
2.4.1.65 S110R site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity Homo sapiens
2.4.1.65 S111K site-directed mutagenesis of FucT-V, the mutant activity is similar to the wild-type enzyme activity Homo sapiens
2.4.1.65 T87K site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme Homo sapiens
2.4.1.65 T87K/D125E site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme Homo sapiens
2.4.1.65 T87K/V89I/A101T insertion mutagenesis of FucT-V, the mutant shows altered substrate specificity compared to the wild-type enzyme Homo sapiens
2.4.1.65 T87K/W124R site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme Homo sapiens
2.4.1.65 T87K/W124R/D125E/I126V site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme Homo sapiens
2.4.1.65 V112I site-directed mutagenesis of FucT-VI, the mutant activity is similar to the wild-type enzyme activity Homo sapiens
2.4.1.65 W124R site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme Homo sapiens
2.4.1.65 W124R/D125E/I126V site-directed mutagenesis of FucT-V, the mutant shows decreased activity compared to the wild-type enzyme Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.65 Mn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.65 GDP-beta-L-fucose + NeuAcalpha2-3Galbeta1-4Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide Homo sapiens the enzyme catalyzes the fucose transfer from GDP-fucose to GlcNAc residues of the sialylated polylactosamine acceptor leading to two isomeric monofucosyl antigens, VIM2 and sialyl-Lex ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.65 Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.65 additional information
-
 relative activities of wild-type and mutant enzymes, overview Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.65 GDP-beta-L-fucose + NeuAcalpha2-3Galbeta1-4Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide the enzyme catalyzes the fucose transfer from GDP-fucose to GlcNAc residues of the sialylated polylactosamine acceptor leading to two isomeric monofucosyl antigens, VIM2 and sialyl-Lex Homo sapiens ?
-
 ?
2.4.1.65 GDP-beta-L-fucose + NeuAcalpha2-3Galbeta1-4Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide the enzyme catalyzes the fucose transfer from GDP-fucose to GlcNAc residues of the sialylated polylactosamine acceptor leading to two isomeric monofucosyl antigens, VIM2 and sialyl-Lex Homo sapiens GDP + NeuAcalpha2-3Galbeta1-4[Fucalpha1-3]Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide i.e. sialyl-Lex, main product of FucT-VI ?
2.4.1.65 GDP-beta-L-fucose + NeuAcalpha2-3Galbeta1-4Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide the enzyme catalyzes the fucose transfer from GDP-fucose to GlcNAc residues of the sialylated polylactosamine acceptor leading to two isomeric monofucosyl antigens, VIM2 and sialyl-Lex Homo sapiens GDP + NeuAcalpha2-3Galbeta1-4[Fucalpha1-3]Glc-NAcbeta1-3Galbeta1-4GlcNAcbeta1-3Galbeta1-4Glcbeta1-ceramide i.e. VIM2, main product of FucT-V ?
2.4.1.65 additional information FucT-III and FucT-V catalyze fucose transfer to Galbeta1-3GlcNAc chain acceptors in an alpha1,4-linkage, these two enzymes have distinct site-specific fucosylation properties, site-specific fucosylation is defined within a 40-amino acid segment containing 8 amino acid differences between the two enzymes, FucT-VI has about 12fold higher activity compared with FucT-V with the Trp124/Arg110 site in the enzymes being responsible primarily for this activity difference, substrate specificities of wild-type and mutant enzymes, overview Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.65 alpha1,3/4-fucosyltransferases-V and -VI
-
 Homo sapiens
2.4.1.65 FucT-V
-
 Homo sapiens
2.4.1.65 FucT-VI
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.65 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.65 6.5
-
 assay at Homo sapiens