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Literature summary extracted from

  • Schoenhofen, I.C.; McNally, D.J.; Vinogradov, E.; Whitfield, D.; Young, N.M.; Dick, S.; Wakarchuk, W.W.; Brisson, J.R.; Logan, S.M.
    Functional characterization of dehydratase/aminotransferase pairs from Helicobacter and Campylobacter: enzymes distinguishing the pseudaminic acid and bacillosamine biosynthetic pathways (2006), J. Biol. Chem., 281, 723-732.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.135 expressed as a His-tagged recombinant protein in Escherichia coli Campylobacter jejuni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.34 0.048
-
 UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose pH 7.7, 37°C Campylobacter jejuni subsp. jejuni
2.6.1.92 0.052
-
 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose pH 7.7, 37°C Campylobacter jejuni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.34 UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + L-glutamate Campylobacter jejuni subsp. jejuni the enzyme (Cj1121c) is involved in biosynthesis of bacillosamine UDP-4-amino-4,6-dideoxy-alpha-D-N-acetyl-D-glucosamine + 2-oxoglutarate
-
 ?
2.6.1.92 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate Campylobacter jejuni the enzyme is involved biosynthesis of pseudaminic acid UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine + 2-oxoglutarate
-
 ?
2.6.1.92 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate Helicobacter pylori the enzyme is involved biosynthesis of pseudaminic acid UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine + 2-oxoglutarate
-
 ?
2.6.1.92 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate Campylobacter jejuni 11168 the enzyme is involved biosynthesis of pseudaminic acid UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine + 2-oxoglutarate
-
 ?
4.2.1.115 2 UDP-N-acetylglucosamine Campylobacter jejuni the Cj1293 enzyme exhibits C6 dehydratase as well as C5 epimerase activity resulting in the production of both UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose and UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose. The enzyme is involved in biosynthesis of pseudaminic acid for glycomodification of the bacterial falgellins, overview UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + 2 H2O
-
 ?
4.2.1.115 2 UDP-N-acetylglucosamine Helicobacter pylori the HP0840 enzyme exhibits C6 dehydratase as well as C5 epimerase activity resulting in the production of both UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose and UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose. The enzyme is involved in biosynthesis of pseudaminic acid for glycomodification of the bacterial falgellins, overview UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + 2 H2O
-
 ?
4.2.1.115 2 UDP-N-acetylglucosamine Campylobacter jejuni 11168 the Cj1293 enzyme exhibits C6 dehydratase as well as C5 epimerase activity resulting in the production of both UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose and UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose. The enzyme is involved in biosynthesis of pseudaminic acid for glycomodification of the bacterial falgellins, overview UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + 2 H2O
-
 ?
4.2.1.115 additional information Campylobacter jejuni the flagellar aminotransferases Cj1294 utilize only UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose as substrate producing UDP-4-amino-4,6-dideoxy-beta-L-AltNAc, a precursor in the Pse biosynthetic pathway ?
-
 ?
4.2.1.115 additional information Helicobacter pylori the flagellar aminotransferases HP0366 utilize only UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose as substrate producing UDP-4-amino-4,6-dideoxy-beta-L-AltNAc, a precursor in the Pse biosynthetic pathway ?
-
 ?
4.2.1.115 additional information Campylobacter jejuni 11168 the flagellar aminotransferases Cj1294 utilize only UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose as substrate producing UDP-4-amino-4,6-dideoxy-beta-L-AltNAc, a precursor in the Pse biosynthetic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.34 Campylobacter jejuni subsp. jejuni Q0P9D3
-
-
2.6.1.92 Campylobacter jejuni
-
-
-
2.6.1.92 Helicobacter pylori O25130
-
-
4.2.1.115 Campylobacter jejuni
-
-
-
4.2.1.115 Campylobacter jejuni 11168
-
-
-
4.2.1.115 Helicobacter pylori
-
-
-
4.2.1.135 Campylobacter jejuni
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.34 UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + L-glutamate the enzyme (Cj1121c) is involved in biosynthesis of bacillosamine Campylobacter jejuni subsp. jejuni UDP-4-amino-4,6-dideoxy-alpha-D-N-acetyl-D-glucosamine + 2-oxoglutarate
-
 ?
2.6.1.34 UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + L-glutamate the enzyme (Cj1121c) does not utilize UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose as substrate Campylobacter jejuni subsp. jejuni UDP-4-amino-4,6-dideoxy-alpha-D-N-acetyl-D-glucosamine + 2-oxoglutarate
-
 ?
2.6.1.92 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate
-
 Helicobacter pylori UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine + 2-oxoglutarate
-
 ?
2.6.1.92 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate the enzyme is involved biosynthesis of pseudaminic acid Campylobacter jejuni UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine + 2-oxoglutarate
-
 ?
2.6.1.92 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate the enzyme is involved biosynthesis of pseudaminic acid Helicobacter pylori UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine + 2-oxoglutarate
-
 ?
2.6.1.92 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate the enzyme (Cj1294) does not utilize UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose as substrate Campylobacter jejuni UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine + 2-oxoglutarate
-
 ?
2.6.1.92 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate the enzyme is involved biosynthesis of pseudaminic acid Campylobacter jejuni 11168 UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine + 2-oxoglutarate
-
 ?
2.6.1.92 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + L-glutamate the enzyme (Cj1294) does not utilize UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose as substrate Campylobacter jejuni 11168 UDP-4-amino-4,6-dideoxy-L-N-acetyl-beta-L-altrosamine + 2-oxoglutarate
-
 ?
4.2.1.115 2 UDP-N-acetylglucosamine the Cj1293 enzyme exhibits C6 dehydratase as well as C5 epimerase activity resulting in the production of both UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose and UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose. The enzyme is involved in biosynthesis of pseudaminic acid for glycomodification of the bacterial falgellins, overview Campylobacter jejuni UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + 2 H2O
-
 ?
4.2.1.115 2 UDP-N-acetylglucosamine the Cj1293 enzyme exhibits C6 dehydratase as well as C5 epimerase activity resulting in the production of both UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose and UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose. The enzyme is involved in biosynthesis of pseudaminic acid for glycomodification of the bacterial falgellins, overview Campylobacter jejuni UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + 2 H2O NMR analysis of reaction products, overview ?
4.2.1.115 2 UDP-N-acetylglucosamine the HP0840 enzyme exhibits C6 dehydratase as well as C5 epimerase activity resulting in the production of both UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose and UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose. The enzyme is involved in biosynthesis of pseudaminic acid for glycomodification of the bacterial falgellins, overview Helicobacter pylori UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + 2 H2O
-
 ?
4.2.1.115 2 UDP-N-acetylglucosamine the HP0840 enzyme exhibits C6 dehydratase as well as C5 epimerase activity resulting in the production of both UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose and UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose. The enzyme is involved in biosynthesis of pseudaminic acid for glycomodification of the bacterial falgellins, overview Helicobacter pylori UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + 2 H2O NMR analysis of reaction products, overview ?
4.2.1.115 2 UDP-N-acetylglucosamine the Cj1293 enzyme exhibits C6 dehydratase as well as C5 epimerase activity resulting in the production of both UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose and UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose. The enzyme is involved in biosynthesis of pseudaminic acid for glycomodification of the bacterial falgellins, overview Campylobacter jejuni 11168 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + 2 H2O
-
 ?
4.2.1.115 2 UDP-N-acetylglucosamine the Cj1293 enzyme exhibits C6 dehydratase as well as C5 epimerase activity resulting in the production of both UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose and UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose. The enzyme is involved in biosynthesis of pseudaminic acid for glycomodification of the bacterial falgellins, overview Campylobacter jejuni 11168 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose + UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose + 2 H2O NMR analysis of reaction products, overview ?
4.2.1.115 additional information the flagellar aminotransferases Cj1294 utilize only UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose as substrate producing UDP-4-amino-4,6-dideoxy-beta-L-AltNAc, a precursor in the Pse biosynthetic pathway Campylobacter jejuni ?
-
 ?
4.2.1.115 additional information the flagellar aminotransferases HP0366 utilize only UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose as substrate producing UDP-4-amino-4,6-dideoxy-beta-L-AltNAc, a precursor in the Pse biosynthetic pathway Helicobacter pylori ?
-
 ?
4.2.1.115 additional information the flagellar aminotransferases Cj1294 utilize only UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose as substrate producing UDP-4-amino-4,6-dideoxy-beta-L-AltNAc, a precursor in the Pse biosynthetic pathway Campylobacter jejuni 11168 ?
-
 ?
4.2.1.135 UDP-N-acetylglucosamine
-
 Campylobacter jejuni UDP-2-acetamido-2,6-dideoxy-alpha-D-4-ketohexulose + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.34 Cj1121c
-
 Campylobacter jejuni subsp. jejuni
2.6.1.34 PglE
-
 Campylobacter jejuni subsp. jejuni
2.6.1.34 UDP-4-keto-6-deoxy-GlcNAc C4 aminotransferase
-
 Campylobacter jejuni subsp. jejuni
2.6.1.92 Cj1294
-
 Campylobacter jejuni
2.6.1.92 HP0366
-
 Helicobacter pylori
4.2.1.115 Cj1293
-
 Campylobacter jejuni
4.2.1.115 FlaA1
-
 Helicobacter pylori
4.2.1.115 HP0840
-
 Helicobacter pylori
4.2.1.115 PseB
-
 Campylobacter jejuni
4.2.1.115 UDP-alpha-D-GlcNAc modifying dehydratase
-
 Helicobacter pylori
4.2.1.115 UDP-alpha-D-GlcNAc modifying dehydratase
-
 Campylobacter jejuni
4.2.1.135 Cj1120c
-
 Campylobacter jejuni
4.2.1.135 PglF
-
 Campylobacter jejuni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.34 37
-
 assay at Campylobacter jejuni subsp. jejuni
2.6.1.92 37
-
 assay at Campylobacter jejuni
2.6.1.92 37
-
 assay at Helicobacter pylori
4.2.1.115 37
-
 assay at Helicobacter pylori
4.2.1.115 37
-
 assay at Campylobacter jejuni
4.2.1.135 7.2
-
 assay at Campylobacter jejuni

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.34 2.4
-
 UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-4-hexulose pH 7.7, 37°C Campylobacter jejuni subsp. jejuni
2.6.1.92 0.067
-
 UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose pH 7.7, 37°C Campylobacter jejuni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.34 7.7
-
 assay at Campylobacter jejuni subsp. jejuni
2.6.1.92 7.7
-
 assay at Campylobacter jejuni
2.6.1.92 7.7
-
 assay at Helicobacter pylori
4.2.1.115 7.2
-
 assay at Helicobacter pylori
4.2.1.115 7.2
-
 assay at Campylobacter jejuni

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.34 pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Campylobacter jejuni subsp. jejuni
2.6.1.92 pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Campylobacter jejuni
2.6.1.92 pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Helicobacter pylori
4.2.1.115 additional information the dehydratase does not require the addition of exogenous cofactor NAD(P)+ Helicobacter pylori
4.2.1.115 additional information the dehydratase does not require the addition of exogenous cofactor NAD(P)+ Campylobacter jejuni

General Information

EC Number General Information Comment Organism
2.6.1.91 physiological function the enzyme (Cj1121c) is involved in biosynthesis of bacillosamine Campylobacter jejuni
4.2.1.135 metabolism PglF is found to possess only C6 dehydratase activity generating UDP-2-acetamido-2,6-dideoxy-alpha-D-4-ketohexulose Campylobacter jejuni