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Literature summary extracted from

  • Kavli, B.; Sundheim, O.; Akbari, M.; Otterlei, M.; Nilsen, H.; Skorpen, F.; Aas, P.A.; Hagen, L.; Krokan, H.E.; Slupphaug, G.
    hUNG2 is the major repair enzyme for removal of uracil from U:A matches, U:G mismatches, and U in single-stranded DNA, with hSMUG1 as a broad specificity backup (2002), J. Biol. Chem., 277, 39926-39936.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.2.27 APE1 has a strong stimulatory effect on hSMUG1 against dsU, apparently because of enhanced dissociation of hSMUG1 from AP sites in dsDNA. AP sites inhibit hSMUG1 Homo sapiens
3.2.2.27 additional information AP sites do not inhibit hUNG2 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.27 expression of hSMUG1 in Escherichia coli strain BL21(DE3) Homo sapiens
3.2.2.27 expression of hUNG2 in Escherichia coli strain BL21 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.27 additional information
-
 additional information UNG2 kinetics Homo sapiens
3.2.2.27 0.0004
-
 uracil-mismatched double-stranded DNA with U-G mismatch pH 7.5, 30°C, recombinant hUNG2 Homo sapiens
3.2.2.27 0.0007
-
 uracil-mismatched double-stranded DNA with U-A mismatch pH 7.5, 30°C, recombinant hUNG2 Homo sapiens
3.2.2.27 0.0013
-
 uracil-mismatched double-stranded DNA with U-G mismatch pH 7.5, 30°C, recombinant hSMUG1 Homo sapiens
3.2.2.27 0.0017
-
 uracil-mismatched single-stranded DNA pH 7.5, 30°C, recombinant hSMUG1 Homo sapiens
3.2.2.27 0.0022
-
 uracil-mismatched single-stranded DNA pH 7.5, 30°C, recombinant hUNG2 Homo sapiens
3.2.2.27 0.0027
-
 5-hydroxymethyluracil-mismatched double-stranded DNA with U-G mismatch pH 7.5, 30°C, recombinant hSMUG1 Homo sapiens
3.2.2.27 0.0038
-
 5-hydroxymethyluracil-mismatched single-stranded DNA pH 7.5, 30°C, recombinant hSMUG1 Homo sapiens
3.2.2.27 0.004
-
 uracil-mismatched double-stranded DNA with U-A mismatch pH 7.5, 30°C, recombinant hSMUG1 Homo sapiens
3.2.2.27 0.0046
-
 5-hydroxymethyluracil-mismatched double-stranded DNA with U-A mismatch pH 7.5, 30°C, recombinant hSMUG1 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.2.27 nucleolus hSMUG1 accumulates in nucleoli in living cells Homo sapiens 5730
-
3.2.2.27 nucleolus hUNG2 is excluded from nucleoli in living cells, but hUNG2 accumulates in replication foci in the S-phase Homo sapiens 5730
-
3.2.2.27 nucleus hUNG2 Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.2.27 Mg2+ strongly stimulating at physiological concentrations Homo sapiens
3.2.2.27 Mg2+ strongly stimulating at physiological concentrations, 10fold for Ung2 at 10 mM. Mg2+ increases the preference of hUNG2 toward uracil in ssDNA nearly 40fold Homo sapiens
3.2.2.27 NaCl hSMUG1 is stimulated by 30% at 70 mM NaCl Homo sapiens
3.2.2.27 NaCl hUNG2 is stimulated 2fold by 50 mM NaCl Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.27 3,N4-ethenocytosine-mismatched single-stranded DNA + H2O Homo sapiens
-
 3,N4-ethenocytosine + single-stranded DNA with abasic site
-
 ?
3.2.2.27 5-hydroxyuracil-mismatched single-stranded DNA + H2O Homo sapiens
-
 5-hydroxyuracil + single-stranded DNA with abasic site
-
 ?
3.2.2.27 additional information Homo sapiens hSMUG1 removes uracil from both double- and single-stranded DNA in nuclear chromatin, hSMUG1 has a broad substrate specificity, including 5-hydroxymethyluracil, and 3,N4-ethenocytosine. hSMUG1 acts as a broad specificity backup and is the major 5-hydroxymethyluracil-DNA glycosylase in nuclear cell extracts, overview ?
-
 ?
3.2.2.27 additional information Homo sapiens hUNG2 removes uracil from both double- and single-stranded DNA in nuclear chromatin. hUNG2 in nuclear extracts initiates base excision repair of plasmids containing either U-A and U-G in vitro. hUNG2 is responsible for both prereplicative removal of deaminated cytosine and postreplicative removal of misincorporated uracil at the replication fork, it is the major enzyme for removal of deaminated cytosine outside of replication foci, overview ?
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O Homo sapiens
-
 uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched single-stranded DNA + H2O Homo sapiens
-
 uracil + single-stranded DNA with abasic site
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.27 Homo sapiens
-
-
-
3.2.2.27 Homo sapiens P22674
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.27 recombinant hSMUG1 from Escherichia coli strain BL21(DE3) by protamine sulfate precipitation, hydrophobic interaction and adsorption chromatography, ultrafiltration, ion exchange chromatography, polyU-affinity, and again ion exchange chromatography. Recombinant hUNG2 from Escherichia coli strain BL21 by gel filtration, ion exchange chromatography, and ultrafiltration Homo sapiens
3.2.2.27 recombinant hUNG2 from Escherichia coli strain BL21 by gel filtration, ion exchange chromatography, and ultrafiltration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.27 colorectal carcinoma cell
-
 Homo sapiens
-
3.2.2.27 CX-1 cell
-
 Homo sapiens
-
3.2.2.27 HaCaT cell
-
 Homo sapiens
-
3.2.2.27 HeLa cell
-
 Homo sapiens
-
3.2.2.27 keratinocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.27 additional information
-
 catalytic efficiencies of hUNG2 and hSMUG1 Homo sapiens
3.2.2.27 additional information
-
 catalytic efficiency of hSMUG1 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.27 3,N4-ethenocytosine-mismatched single-stranded DNA + H2O
-
 Homo sapiens 3,N4-ethenocytosine + single-stranded DNA with abasic site
-
 ?
3.2.2.27 5-hydroxymethyluracil-mismatched double-stranded DNA with U-A mismatch + H2O
-
 Homo sapiens 5-hydroxymethyluracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 5-hydroxymethyluracil-mismatched double-stranded DNA with U-G mismatch + H2O
-
 Homo sapiens 5-hydroxymethyluracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 5-hydroxymethyluracil-mismatched single-stranded DNA + H2O
-
 Homo sapiens 5-hydroxymethyluracil + single-stranded DNA with abasic site
-
 ?
3.2.2.27 5-hydroxyuracil-mismatched single-stranded DNA + H2O
-
 Homo sapiens 5-hydroxyuracil + single-stranded DNA with abasic site
-
 ?
3.2.2.27 additional information substrate specificity, overview Homo sapiens ?
-
 ?
3.2.2.27 additional information hSMUG1 removes uracil from both double- and single-stranded DNA in nuclear chromatin, hSMUG1 has a broad substrate specificity, including 5-hydroxymethyluracil, and 3,N4-ethenocytosine. hSMUG1 acts as a broad specificity backup and is the major 5-hydroxymethyluracil-DNA glycosylase in nuclear cell extracts, overview Homo sapiens ?
-
 ?
3.2.2.27 additional information hUNG2 removes uracil from both double- and single-stranded DNA in nuclear chromatin. hUNG2 in nuclear extracts initiates base excision repair of plasmids containing either U-A and U-G in vitro. hUNG2 is responsible for both prereplicative removal of deaminated cytosine and postreplicative removal of misincorporated uracil at the replication fork, it is the major enzyme for removal of deaminated cytosine outside of replication foci, overview Homo sapiens ?
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O
-
 Homo sapiens uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA with U-A mismatch + H2O
-
 Homo sapiens uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched double-stranded DNA with U-G mismatch + H2O
-
 Homo sapiens uracil + double-stranded DNA with abasic site
-
 ?
3.2.2.27 uracil-mismatched single-stranded DNA + H2O
-
 Homo sapiens uracil + single-stranded DNA with abasic site
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.27 single-strand selective monofunctional uracil-DNA glycosylase
-
 Homo sapiens
3.2.2.27 SMUG1
-
 Homo sapiens
3.2.2.27 UNG2
-
 Homo sapiens
3.2.2.27 uracil-DNA glycosylase 2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.27 30
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.27 0.015
-
 5-hydroxymethyluracil-mismatched double-stranded DNA with U-A mismatch pH 7.5, 30°C, recombinant hSMUG1 Homo sapiens
3.2.2.27 0.04
-
 5-hydroxymethyluracil-mismatched double-stranded DNA with U-G mismatch pH 7.5, 30°C, recombinant hSMUG1 Homo sapiens
3.2.2.27 0.052
-
 uracil-mismatched double-stranded DNA with U-A mismatch pH 7.5, 30°C, recombinant hSMUG1 Homo sapiens
3.2.2.27 0.055
-
 uracil-mismatched double-stranded DNA with U-G mismatch pH 7.5, 30°C, recombinant hSMUG1 Homo sapiens
3.2.2.27 0.098
-
 5-hydroxymethyluracil-mismatched single-stranded DNA pH 7.5, 30°C, recombinant hSMUG1 Homo sapiens
3.2.2.27 0.29
-
 uracil-mismatched single-stranded DNA pH 7.5, 30°C, recombinant hSMUG1 Homo sapiens
3.2.2.27 2.28
-
 uracil-mismatched double-stranded DNA with U-A mismatch pH 7.5, 30°C, recombinant hUNG2 Homo sapiens
3.2.2.27 5.57
-
 uracil-mismatched double-stranded DNA with U-G mismatch pH 7.5, 30°C, recombinant hUNG2 Homo sapiens
3.2.2.27 46.1
-
 uracil-mismatched single-stranded DNA pH 7.5, 30°C, recombinant hUNG2 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.27 7 7.5 hSMUG1 Homo sapiens
3.2.2.27 7 7.5 hUNG2 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.2.27 additional information
-
 pH profile of hSMUG1 Homo sapiens
3.2.2.27 additional information
-
 pH profile of hUNG2 Homo sapiens