Literature summary extracted from
Karow, J.K.; Chakraverty, R.K.
Hickson, I.D.: The Bloom's syndrome gene product is a 3'-5' DNA helicase (1997), J. Biol. Chem., 272, 30611-30604.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
5.6.2.4 |
Double-stranded DNA |
the enzyme is strongly stimulated by either single- or double-stranded DNA |
Homo sapiens |
|
5.6.2.4 |
single-stranded DNA |
the enzyme is strongly stimulated by either single- or double-stranded DNA |
Homo sapiens |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.6.2.4 |
overexpression of an oligohistidine-tagged version of the BLM gene product in Saccharomyces cerevisiae |
Homo sapiens |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
5.6.2.4 |
Mg2+ |
required |
Homo sapiens |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
5.6.2.4 |
180000 |
- |
x * 180000, SDS-PAGE |
Homo sapiens |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.6.2.4 |
Homo sapiens |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.6.2.4 |
recombinant |
Homo sapiens |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
5.6.2.4 |
B-cell |
- |
Homo sapiens |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.6.2.4 |
ATP + H2O |
the enzyme unwinds DNA in the 3'-5' direction with respect to the strand to which the enzyme is bound |
Homo sapiens |
ADP + phosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
5.6.2.4 |
? |
x * 180000, SDS-PAGE |
Homo sapiens |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.6.2.4 |
BLM |
- |
Homo sapiens |
5.6.2.4 |
BLM protein |
- |
Homo sapiens |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
5.6.2.4 |
37 |
- |
assay at |
Homo sapiens |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
5.6.2.4 |
7.5 |
- |
assay at |
Homo sapiens |