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Literature summary extracted from

  • Oboh, O.T.; Lamango, N.S.
    Liver prenylated methylated protein methyl esterase is the same enzyme as Sus scrofa carboxylesterase (2008), J. Biochem. Mol. Toxicol., 22, 51-62.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 (3S)-1-chloro-3-tosylamido-7-amino-2-heptanone 73.6% residual activity at 1 mM Sus scrofa
3.1.1.1 asulam 91.7% residual activity at 1 mM Sus scrofa
3.1.1.1 benzil 38% residual activity at 1 mM Sus scrofa
3.1.1.1 ebelactone A inhibits more than 80% of the enzyme at 1 mM Sus scrofa
3.1.1.1 ebelactone B complete inhibition at 1 mM Sus scrofa
3.1.1.1 L-4-tosylamino-2-phenylethyl chloromethyl ketone inhibits more than 80% of the enzyme at 1 mM Sus scrofa
3.1.1.1 L-Leu-chloromethyl ketone 60.4% residual activity at 1 mM Sus scrofa
3.1.1.1 additional information not inhibited by benzyl carbamate Sus scrofa
3.1.1.1 N-(methoxysuccinyl)-L-Ala-L-Ala-L-Pro-L-Val chloromethyl ketone 61.8% residual activity at 1 mM Sus scrofa
3.1.1.1 N-alpha-tosyl-L-Lys chloromethyl ketone 98.6% residual activity at 1 mM Sus scrofa
3.1.1.1 N-carbobenzyloxy-L-Phe chloromethyl ketone 57% residual activity at 1 mM Sus scrofa
3.1.1.1 paraoxon complete inhibition at 1 mM Sus scrofa
3.1.1.1 phenylmethylsulfonyl fluoride complete inhibition at 1 mM Sus scrofa
3.1.1.1 tosyl-L-phenylalanine chloromethyl ketone inhibits more than 80% of the enzyme at 1 mM Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.025
-
hydrocinnamoyl farnesyl-L-cysteine methyl ester
-
Sus scrofa
3.1.1.1 0.033
-
benzoyl-Gly-farnesyl-L-cysteine methyl ester
-
Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 57000
-
SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 DEAE ion-exchange column chromatography, Q-Sepharose column chromatography, phenyl Sepharose column chromatography, Superdex 200 gel filtration, and Cu2+-charged immobilized metal ion affinity chromatography Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.1 liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 0.0004
-
crude extract Sus scrofa
3.1.1.1 0.00529
-
after 13.23fold purification Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl acetate + H2O
-
Sus scrofa 4-nitrophenol + acetate
-
?
3.1.1.1 benzoyl-Gly-farnesyl-L-cysteine methyl ester + H2O
-
Sus scrofa ?
-
?
3.1.1.1 hydrocinnamoyl farnesyl-L-cysteine methyl ester + H2O
-
Sus scrofa N-(3-phenylpropanoyl)-S-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]-L-cysteine + methanol
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 PPMTase
-
Sus scrofa
3.1.1.1 prenylated methylated protein methyl esterase
-
Sus scrofa