Literature summary extracted from

Lee, H.J.; Jang, do S.; Cha, H.J.; Moon, H.S.; Hong, B.H.; Choi, K.Y.; Lee, H.C.
NMR studies on the equilibrium unfolding of ketosteroid isomerase by urea (2008), J. Biochem., 144, 215-221.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
5.3.3.1	overexpression in Escherichia coli	Pseudomonas putida

Organism

EC Number	Organism	UniProt	Comment	Textmining
5.3.3.1	Pseudomonas putida	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
5.3.3.1	15N labelled KSI is purified from Escherichia coli BL21 DE3 grown in minimal medium (M9) containing 15N ammonium chloride as the nitrogen source.	Pseudomonas putida

Renatured (Commentary)

EC Number	Renatured (Comment)	Organism
5.3.3.1	secondary structure is not significantly affected by urea up to 3.5 M, but the residues in the dimeric interface region are significantly perturbed by urea at low concentrations. The interface region primarily around the beta5- and beta6-strands may play an important role as the starting positions in the unfolding process	Pseudomonas putida

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
5.3.3.1	cell culture	biotype B	Pseudomonas putida	-

Subunits

EC Number	Subunits	Comment	Organism
5.3.3.1	homodimer	determined by 3D NMR, the secondary and tertiary structures of KSI are not affected significantly by urea up to 3.5 M. The chemical shift analysis of 1H-15N HSQC spectra at various urea concentrations reveales that the residues in the dimeric interface region, particularly around the beta5-strand, are significantly perturbed by urea at low concentrations, while the line-width analysis indicates the possibility of conformational exchange at the interface region around the beta6-strand. The interface region primarily around the beta5- and beta6-strands could play an important role as the starting positions in the unfolding process of KSI.	Pseudomonas putida

Synonyms

EC Number	Synonyms	Comment	Organism
5.3.3.1	ketosteroid isomerase	-	Pseudomonas putida
5.3.3.1	KSI	-	Pseudomonas putida

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Release 2023.1 (January 2023)