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Literature summary extracted from

  • Grosse, S.; Voigt, C.; Wendlandt, K.D.; Kleber, H.P.
    Purification and properties of methanol dehydrogenase from Methylosinus sp. WI 14 (1998), J. Basic Microbiol., 8, 189-196.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.2.B2 70000
-
 2 * 70000, SDS-PAGE Methylocystis sp.
1.2.2.B2 140000
-
 gel filtration Methylocystis sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.2.B2 Methylocystis sp.
-
-
-
1.2.2.B2 Methylocystis sp. WI14
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.2.B2
-
 Methylocystis sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.2.B2 6.12
-
-
 Methylocystis sp.

Storage Stability

EC Number Storage Stability Organism
1.2.2.B2 -18°C, solutions of the purified enzyme stored in 0.05 M sodium phosphate buffer, pH 7.5, show about 10% loss of activity during 24 h Methylocystis sp.
1.2.2.B2 4°C, solutions of the purified enzyme stored in 0.05 M sodium phosphate buffer, pH 7.5, show about 30% loss of activity during 24 h Methylocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.2.B2 acetaldehyde + oxidized 2,6-dichlorophenolindophenol
-
 Methylocystis sp. acetate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.2.B2 acetaldehyde + oxidized 2,6-dichlorophenolindophenol
-
 Methylocystis sp. WI14 acetate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.2.B2 formaldehyde + oxidized 2,6-dichlorophenolindophenol
-
 Methylocystis sp. formate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.2.B2 formaldehyde + oxidized 2,6-dichlorophenolindophenol
-
 Methylocystis sp. WI14 formate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.2.B2 additional information the enzyme from Methylosinus sp. WI 14 oxidizes only primary alcohols up to octanol and several aldehydes Methylocystis sp. ?
-
 ?
1.2.2.B2 additional information the enzyme from Methylosinus sp. WI 14 oxidizes only primary alcohols up to octanol and several aldehydes Methylocystis sp. WI14 ?
-
 ?
1.2.2.B2 propionaldehyde + oxidized 2,6-dichlorophenolindophenol
-
 Methylocystis sp. propionate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.2.2.B2 propionaldehyde + oxidized 2,6-dichlorophenolindophenol
-
 Methylocystis sp. WI14 propionate + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.2.B2 homodimer 2 * 70000, SDS-PAGE Methylocystis sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.2.B2 pyrroloquinoline quinone 0.65 mole per subunit Methylocystis sp.