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Literature summary extracted from

  • Yan, R.; Hou, J.; Ding, D.; Guan, W.; Wang, C.; Wu, Z.; Li, M.
    In vitro antifungal activity and mechanism of action of chitinase against four plant pathogenic fungi (2008), J. Basic Microbiol., 48, 293-301.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 subcloning in Escherichia coli strain DH5alpha, expression in Pichia pastoris strain GS115 Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Oryza sativa chitin from fungal cell wall, the chitinase exhibits different antifungal activities against the four fungi, which is directly correlated to the surface microstructure and the proportion of chitin in the fungal cell wall, antifungal mechanism of action of chitinase against four plant pathogenic fungi, i.e. Rhizopus stolonifer, Botrytis squamosa, Pythium aphanidermatum, and Aspergillus niger, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Oryza sativa P24626
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-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 recombinant enzyme 6fold from Pichia pastoris Oryza sativa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 additional information
-
 purified recombinant enzyme, and antifungal activities, overview Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
-
 Oryza sativa ?
-
 ?
3.2.1.14 chitin + H2O chitin from fungal cell wall, the chitinase exhibits different antifungal activities against the four fungi, which is directly correlated to the surface microstructure and the proportion of chitin in the fungal cell wall, antifungal mechanism of action of chitinase against four plant pathogenic fungi, i.e. Rhizopus stolonifer, Botrytis squamosa, Pythium aphanidermatum, and Aspergillus niger, overview Oryza sativa ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 25
-
 in vivo assay at Oryza sativa