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Literature summary extracted from
- An essential DnaB helicase of Bacillus anthracis: identification, characterization, and mechanism of action (2009), J. Bacteriol., 191, 249-260.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.6.2.3 | - |
Bacillus anthracis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.3 | M13mp19 ssDNA | ATPase activity is slightly stimulated by ssDNA, and only M13mp19 ssDNA stimulates it significantly (increase in Vmax) | Bacillus anthracis | |
| 5.6.2.3 | ssDNA | ATPase activity is slightly stimulated by ssDNA, and only M13mp19 ssDNA stimulats it significantly | Bacillus anthracis |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.6.2.3 | 0.152 | - |
ATP | pH 7.5, 37°C, addition of 60-mer oligonucleotide | Bacillus anthracis | |
| 5.6.2.3 | 0.22 | - |
ATP | pH 7.5, 37°C, addition of M13mp19 ssDNA | Bacillus anthracis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.6.2.3 | 50000 | - |
x * 50000, SDS-PAGE | Bacillus anthracis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.6.2.3 | Bacillus anthracis | Q81JI8 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.6.2.3 | - |
Bacillus anthracis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.2.3 | ATP + H2O | strong 5' to 3' DNA helicase activity. At both 0.1 and 0.5 mM, dATP produces comparable or slightly higher levels of unwinding than ATP | Bacillus anthracis | ADP + phosphate | - |
? | |
| 5.6.2.3 | dATP + H2O | strong 5' to 3' DNA helicase activity. At both 0.1 and 0.5 mM, dATP produces comparable or slightly higher levels of unwinding than ATP | Bacillus anthracis | dADP + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.6.2.3 | ? | x * 50000, SDS-PAGE | Bacillus anthracis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.6.2.3 | dnaB | - |
Bacillus anthracis |
| 5.6.2.3 | DnaB helicase | - |
Bacillus anthracis |
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Release 2023.1 (January 2023)
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