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Literature summary extracted from

  • Hagemann, M.; Ribbeck-Busch, K.; Klaehn, S.; Hasse, D.; Steinbruch, R.; Berg, G.
    The plant-associated bacterium Stenotrophomonas rhizophila expresses a new enzyme for the synthesis of the compatible solute glucosylglycerol (2008), J. Bacteriol., 190, 5898-5906.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.213 additional information gene ggpPS is induced by salt shock Stenotrophomonas rhizophila

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.213 gene ggpPS, DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and truncated mutant enzymes in Escherichia coli strain LMG194, complementation of the glucosylglycerol-defective mutants of the cyanobacterium Synechocystis sp. strain PCC 6803 Stenotrophomonas rhizophila
3.1.3.69 expressed in Escherichia coli strain LMG194 Stenotrophomonas rhizophila

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.213 additional information construction of a truncated enzyme version devoid of the phosphatase part, which synthesizes glucosylglycerol-phosphate, in contrast to the full-length protein, that synthesizes glucosylglycerol, dephosphorylation of glucosylglycerol phosphate is detected only with the complete GgpPS protein, overview. Expression of the full-length ggpPS gene leads to complementation of the salt-sensitive phenotype of the mutant defective in the phosphatase step showing a severe drop in salt resistance because of the toxic effect of glucosylglycerol phosphate accumulation Stenotrophomonas rhizophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.213 Mg2+
-
 Stenotrophomonas rhizophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.213 additional information Stenotrophomonas rhizophila the rhizobacterium Stenotrophomonas rhizophila accumulates the compatible solutes glucosylglycerol and trehalose under salt stress conditions, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.213 Stenotrophomonas rhizophila Q5GMP2 a plant associated bacterium, gene ggpPS
-
3.1.3.69 Stenotrophomonas rhizophila Q5GMP2 strain DSM 14405
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.213 recombinant His-tagged wild-type and truncated mutant enzymes from Escherichia coli strain LMG194 by nickel affinity chromatography Stenotrophomonas rhizophila
3.1.3.69 Ni-NTA column chromatography Stenotrophomonas rhizophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.213 additional information the rhizobacterium Stenotrophomonas rhizophila accumulates the compatible solutes glucosylglycerol and trehalose under salt stress conditions, overview Stenotrophomonas rhizophila ?
-
 ?
2.4.1.213 additional information a truncated enzyme version, devoid of the phosphatase part, synthesizes glucosylglycerol-phosphate, in contrast to the full-length wild-type enzyme, that synthesizes glucosylglycerol. The complete GgpPS protein also catalyzes dephosphorylation of glucosylglycerol phosphate, overview Stenotrophomonas rhizophila ?
-
 ?
3.1.3.69 2-(beta-D-glucosyl)-sn-glycerol-3-phosphate + H2O
-
 Stenotrophomonas rhizophila 2-(beta-D-glucosyl)-sn-glycerol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.213 More the enzyme shows a C-terminal GG-phosphate synthase domain and an N-terminal putative GG-phosphate phosphatase domain Stenotrophomonas rhizophila

Synonyms

EC Number Synonyms Comment Organism
2.4.1.213 GGPPS
-
 Stenotrophomonas rhizophila
3.1.3.69 GG-phosphate phosphatase
-
 Stenotrophomonas rhizophila
3.1.3.69 GGPPS composed of an N-terminal glucosylglycerol phosphatase domain fused with a C-terminal glucosylglycerol synthase domain Stenotrophomonas rhizophila
3.1.3.69 glucosylglycerol phosphate phosphatase
-
 Stenotrophomonas rhizophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.213 30
-
 assay at Stenotrophomonas rhizophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.213 7.8
-
 assay at Stenotrophomonas rhizophila