EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.336 | tris-(2-carboxyethyl)phosphine hydrochloride | 1 mM, about 25% increase in activity | Methanococcus maripaludis | |
5.1.3.14 | KCl | 200 mM, twofold stimualtion | Methanococcus maripaludis | |
5.4.2.10 | D-fructose-1,6-bisphosphate | required to activate the enzyme | Methanococcus maripaludis | |
5.4.2.10 | dithiothreitol | 1 mM, required for maximal activity | Methanococcus maripaludis | |
5.4.2.10 | additional information | no activity is observed with ATP as an activator | Methanococcus maripaludis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.336 | expression in Escherichia coli | Methanococcus maripaludis |
5.1.3.14 | expression in Escherichia coli, C-terminal His-tag | Methanococcus maripaludis |
5.4.2.10 | expressed in Escherichia coli BL21(DE3) cells | Methanococcus maripaludis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.336 | N-acetylglucosamine | 1.3 mM, 50% inhibition | Methanococcus maripaludis | |
1.1.1.336 | N-acetylmannosamine | 0.07 mM, 50% inhibition | Methanococcus maripaludis | |
1.1.1.336 | UDP | 0.5 mM, 50% inhibition | Methanococcus maripaludis | |
1.1.1.336 | UDP-glucose | 0.6 mM, 50% inhibition | Methanococcus maripaludis | |
1.1.1.336 | UTP | 1 mM, 50% inhibition | Methanococcus maripaludis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.336 | 0.57 | - |
NAD+ | pH 8.5, 37°C | Methanococcus maripaludis | |
5.1.3.14 | additional information | - |
additional information | Hill coefficient of 2.7, positive cooperativity | Methanococcus maripaludis | |
5.1.3.14 | 0.36 | - |
UDP-N-acetyl-D-glucosamine | pH 8.5, 37°C | Methanococcus maripaludis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.336 | additional information | salts or divalent cations are not required | Methanococcus maripaludis | |
5.4.2.10 | Mg2+ | activity is 95% lower when MgCl2 is used instead of MnCl2 | Methanococcus maripaludis | |
5.4.2.10 | Mn2+ | required for activity | Methanococcus maripaludis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.336 | 50000 | - |
4 * 50000, SDS-PAGE, 4 * 51400, calculated, including His-tag | Methanococcus maripaludis |
1.1.1.336 | 51400 | - |
4 * 50000, SDS-PAGE, 4 * 51400, calculated, including His-tag | Methanococcus maripaludis |
1.1.1.336 | 200000 | - |
gel filtration | Methanococcus maripaludis |
5.1.3.14 | 40000 | - |
2 * 40000, SDS-PAGE, 2 * 42400, calculated, recombinant enzyme | Methanococcus maripaludis |
5.1.3.14 | 42400 | - |
2 * 40000, SDS-PAGE, 2 * 42400, calculated, recombinant enzyme | Methanococcus maripaludis |
5.1.3.14 | 101000 | - |
gel filtration | Methanococcus maripaludis |
5.4.2.10 | 50000 | - |
SDS-PAGE | Methanococcus maripaludis |
5.4.2.10 | 340000 | - |
gel filtration | Methanococcus maripaludis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.336 | Methanococcus maripaludis | Q6LZC3 | - |
- |
5.1.3.14 | Methanococcus maripaludis | Q6LZC4 | - |
- |
5.4.2.10 | Methanococcus maripaludis | Q6LYB4 | strain 900 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.2.10 | Ni+ affinity column chromatography | Methanococcus maripaludis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.336 | additional information | no substrate: UDP-glucose or N-acetyl-D-mannosamine | Methanococcus maripaludis | ? | - |
? | |
1.1.1.336 | UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O | - |
Methanococcus maripaludis | UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH + 2 H+ | ratio of 1.95 NAD molecules consumed per UDP-N-acetyl-alpha-D-mannosaminuronate molecule produced, 4-electron oxidation | ? | |
5.1.3.14 | UDP-N-acetyl-D-glucosamine | - |
Methanococcus maripaludis | UDP-N-acetyl-D-mannosamine | - |
r | |
5.4.2.10 | alpha-D-glucosamine 6-phosphate | - |
Methanococcus maripaludis | alpha-D-glucosamine 1-phosphate | - |
r | |
5.4.2.10 | alpha-D-glucose 1-phosphate | - |
Methanococcus maripaludis | alpha-D-glucose 6-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.336 | tetramer | 4 * 50000, SDS-PAGE, 4 * 51400, calculated, including His-tag | Methanococcus maripaludis |
5.1.3.14 | dimer | 2 * 40000, SDS-PAGE, 2 * 42400, calculated, recombinant enzyme | Methanococcus maripaludis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.336 | MMP0706 | - |
Methanococcus maripaludis |
1.1.1.336 | wecC | - |
Methanococcus maripaludis |
5.4.2.10 | GlmM | - |
Methanococcus maripaludis |
5.4.2.10 | MMP1077 | - |
Methanococcus maripaludis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.1.3.14 | 50 | - |
- |
Methanococcus maripaludis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.336 | 0.4 | - |
NAD+ | pH 8.5, 37°C | Methanococcus maripaludis | |
5.1.3.14 | 3.4 | - |
UDP-N-acetyl-D-glucosamine | pH 8.5, 37°C | Methanococcus maripaludis | |
5.4.2.10 | 0.1 | - |
alpha-D-glucose 1-phosphate | - |
Methanococcus maripaludis | |
5.4.2.10 | 0.52 | - |
alpha-D-glucosamine 1-phosphate | - |
Methanococcus maripaludis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.1.3.14 | 6 | 10 | - |
Methanococcus maripaludis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.336 | additional information | no cofactor: NADP+ | Methanococcus maripaludis | |
1.1.1.336 | NAD+ | - |
Methanococcus maripaludis |