Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Namboori, S.C.; Graham, D.E.
    Acetamido sugar biosynthesis in the Euryarchaea (2008), J. Bacteriol., 190, 2987-2996.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.336 tris-(2-carboxyethyl)phosphine hydrochloride 1 mM, about 25% increase in activity Methanococcus maripaludis
5.1.3.14 KCl 200 mM, twofold stimualtion Methanococcus maripaludis
5.4.2.10 D-fructose-1,6-bisphosphate required to activate the enzyme Methanococcus maripaludis
5.4.2.10 dithiothreitol 1 mM, required for maximal activity Methanococcus maripaludis
5.4.2.10 additional information no activity is observed with ATP as an activator Methanococcus maripaludis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.336 expression in Escherichia coli Methanococcus maripaludis
5.1.3.14 expression in Escherichia coli, C-terminal His-tag Methanococcus maripaludis
5.4.2.10 expressed in Escherichia coli BL21(DE3) cells Methanococcus maripaludis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.336 N-acetylglucosamine 1.3 mM, 50% inhibition Methanococcus maripaludis
1.1.1.336 N-acetylmannosamine 0.07 mM, 50% inhibition Methanococcus maripaludis
1.1.1.336 UDP 0.5 mM, 50% inhibition Methanococcus maripaludis
1.1.1.336 UDP-glucose 0.6 mM, 50% inhibition Methanococcus maripaludis
1.1.1.336 UTP 1 mM, 50% inhibition Methanococcus maripaludis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.336 0.57
-
 NAD+ pH 8.5, 37°C Methanococcus maripaludis
5.1.3.14 additional information
-
 additional information Hill coefficient of 2.7, positive cooperativity Methanococcus maripaludis
5.1.3.14 0.36
-
 UDP-N-acetyl-D-glucosamine pH 8.5, 37°C Methanococcus maripaludis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.336 additional information salts or divalent cations are not required Methanococcus maripaludis
5.4.2.10 Mg2+ activity is 95% lower when MgCl2 is used instead of MnCl2 Methanococcus maripaludis
5.4.2.10 Mn2+ required for activity Methanococcus maripaludis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.336 50000
-
 4 * 50000, SDS-PAGE, 4 * 51400, calculated, including His-tag Methanococcus maripaludis
1.1.1.336 51400
-
 4 * 50000, SDS-PAGE, 4 * 51400, calculated, including His-tag Methanococcus maripaludis
1.1.1.336 200000
-
 gel filtration Methanococcus maripaludis
5.1.3.14 40000
-
 2 * 40000, SDS-PAGE, 2 * 42400, calculated, recombinant enzyme Methanococcus maripaludis
5.1.3.14 42400
-
 2 * 40000, SDS-PAGE, 2 * 42400, calculated, recombinant enzyme Methanococcus maripaludis
5.1.3.14 101000
-
 gel filtration Methanococcus maripaludis
5.4.2.10 50000
-
 SDS-PAGE Methanococcus maripaludis
5.4.2.10 340000
-
 gel filtration Methanococcus maripaludis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.336 Methanococcus maripaludis Q6LZC3
-
-
5.1.3.14 Methanococcus maripaludis Q6LZC4
-
-
5.4.2.10 Methanococcus maripaludis Q6LYB4 strain 900
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.10 Ni+ affinity column chromatography Methanococcus maripaludis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.336 additional information no substrate: UDP-glucose or N-acetyl-D-mannosamine Methanococcus maripaludis ?
-
 ?
1.1.1.336 UDP-N-acetyl-alpha-D-mannosamine + 2 NAD+ + H2O
-
 Methanococcus maripaludis UDP-N-acetyl-alpha-D-mannosaminuronate + 2 NADH + 2 H+ ratio of 1.95 NAD molecules consumed per UDP-N-acetyl-alpha-D-mannosaminuronate molecule produced, 4-electron oxidation ?
5.1.3.14 UDP-N-acetyl-D-glucosamine
-
 Methanococcus maripaludis UDP-N-acetyl-D-mannosamine
-
 r
5.4.2.10 alpha-D-glucosamine 6-phosphate
-
 Methanococcus maripaludis alpha-D-glucosamine 1-phosphate
-
 r
5.4.2.10 alpha-D-glucose 1-phosphate
-
 Methanococcus maripaludis alpha-D-glucose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.336 tetramer 4 * 50000, SDS-PAGE, 4 * 51400, calculated, including His-tag Methanococcus maripaludis
5.1.3.14 dimer 2 * 40000, SDS-PAGE, 2 * 42400, calculated, recombinant enzyme Methanococcus maripaludis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.336 MMP0706
-
 Methanococcus maripaludis
1.1.1.336 wecC
-
 Methanococcus maripaludis
5.4.2.10 GlmM
-
 Methanococcus maripaludis
5.4.2.10 MMP1077
-
 Methanococcus maripaludis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.3.14 50
-
-
 Methanococcus maripaludis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.336 0.4
-
 NAD+ pH 8.5, 37°C Methanococcus maripaludis
5.1.3.14 3.4
-
 UDP-N-acetyl-D-glucosamine pH 8.5, 37°C Methanococcus maripaludis
5.4.2.10 0.1
-
 alpha-D-glucose 1-phosphate
-
 Methanococcus maripaludis
5.4.2.10 0.52
-
 alpha-D-glucosamine 1-phosphate
-
 Methanococcus maripaludis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.3.14 6 10
-
 Methanococcus maripaludis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.336 additional information no cofactor: NADP+ Methanococcus maripaludis
1.1.1.336 NAD+
-
 Methanococcus maripaludis