EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.1.17 | gene tcdB, phylogenetic analysis of threonine ammonia-lyases | Geobacter sulfurreducens |
4.3.1.19 | gene tcdB, phylogenetic analysis of threonine ammonia-lyases | Geobacter sulfurreducens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.3.22 | DLCR6 (cimA::Gm) | citramalate synthase knockout mutant | Geobacter sulfurreducens |
4.1.3.22 | DLCR7 (tdcB::Kn cimA::Gm) | double (ammonia-lyase (EC 4.3.1.19) and citramalate synthase) knockout mutant. DLCR5 (tdcB::Kn) a threonine ammonia-lyase knockout mutant (EC 4.3.1.19) is also constructed | Geobacter sulfurreducens |
4.1.3.22 | additional information | strain DL1 used to construct strains. Construction of mutants via single-step gene replacement. Mutants lacking both enzymes (citramalate synthase and threonine ammonia-lyase) are auxotrophs for isoleucine, whereas single mutants are capable of growth in the absence of isoleucine | Geobacter sulfurreducens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.17 | additional information | TcdB is not inhibited by isoleucine | Geobacter sulfurreducens | |
4.3.1.19 | additional information | TcdB is not inhibited by isoleucine | Geobacter sulfurreducens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.21 | acetyl-CoA + pyruvate + H2O | Geobacter sulfurreducens | - |
CoA + (2S)-2-hydroxy-2-methylbutanedioate | - |
r | |
4.1.3.22 | acetate + pyruvate | Geobacter sulfurreducens | citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM | citramalate | - |
? | |
4.1.3.22 | acetate + pyruvate | Geobacter sulfurreducens ATCC 51573 / DSM 12127 | citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM | citramalate | - |
? | |
4.1.3.22 | acetate + pyruvate | Geobacter sulfurreducens DSM 12127 | citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM | citramalate | - |
? | |
4.3.1.17 | L-serine | Geobacter sulfurreducens | - |
pyruvate + NH3 | - |
? | |
4.3.1.17 | L-serine | Geobacter sulfurreducens ATCC 51573 / DSM 12127 | - |
pyruvate + NH3 | - |
? | |
4.3.1.17 | L-serine | Geobacter sulfurreducens DSM 12127 | - |
pyruvate + NH3 | - |
? | |
4.3.1.19 | L-threonine | Geobacter sulfurreducens | a step in the pathway for the biosynthesis of isoleucine via isoleucine precursor 2-oxobutanoate generated from threonine, this pathway accounts for a minor fraction of isoleucine biosynthesis, while the majority of isoleucine is instead derived from acetyl-coenzyme A and pyruvate, possibly via the citramalate pathway, overview | 2-oxobutanoate + NH3 | - |
? | |
4.3.1.19 | L-threonine | Geobacter sulfurreducens DSM 12127 | a step in the pathway for the biosynthesis of isoleucine via isoleucine precursor 2-oxobutanoate generated from threonine, this pathway accounts for a minor fraction of isoleucine biosynthesis, while the majority of isoleucine is instead derived from acetyl-coenzyme A and pyruvate, possibly via the citramalate pathway, overview | 2-oxobutanoate + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.21 | Geobacter sulfurreducens | Q74C76 | 2-isopropylmalate synthase | - |
4.1.3.22 | Geobacter sulfurreducens | - |
- |
- |
4.1.3.22 | Geobacter sulfurreducens ATCC 51573 / DSM 12127 | - |
- |
- |
4.3.1.17 | Geobacter sulfurreducens | - |
strain DL-1, ATCC 51573, gene GSU0486 or tcdB | - |
4.3.1.17 | Geobacter sulfurreducens DSM 12127 | - |
strain DL-1, ATCC 51573, gene GSU0486 or tcdB | - |
4.3.1.19 | Geobacter sulfurreducens | Q74FW6 | strain DL-1, ATCC 51573, gene GSU0486 or tcdB | - |
4.3.1.19 | Geobacter sulfurreducens DSM 12127 | Q74FW6 | strain DL-1, ATCC 51573, gene GSU0486 or tcdB | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.3.21 | 0.0004 | - |
strain DLCR6, cimA::Gmr | Geobacter sulfurreducens |
2.3.3.21 | 0.0004 | - |
strain DLCR7, tdcB::Knr cimA::Gmr | Geobacter sulfurreducens |
2.3.3.21 | 0.0051 | - |
strain DLCR5, tdcB::Knr | Geobacter sulfurreducens |
2.3.3.21 | 0.0074 | - |
strain DL1, wild type | Geobacter sulfurreducens |
4.1.3.22 | additional information | - |
enzymatic activity in mutant strain DLCR5 (tdcB::Kn), a threonine ammonia-lyase knockout mutant (EC 4.3.1.19), is 0.0051 micromol/min/mg | Geobacter sulfurreducens |
4.1.3.22 | 0.0004 | - |
in mutant strain DLCR6 (cimA::Gm) | Geobacter sulfurreducens |
4.1.3.22 | 0.0004 | - |
in mutant strain DLCR7 (tdcB::Kn cimA::Gm) | Geobacter sulfurreducens |
4.1.3.22 | 0.0074 | - |
DL1 (wild type) | Geobacter sulfurreducens |
4.3.1.17 | 0.041 | - |
strain DL-1 | Geobacter sulfurreducens |
4.3.1.19 | 0.189 | - |
strain DL-1 | Geobacter sulfurreducens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.21 | acetyl-CoA + pyruvate + H2O | - |
Geobacter sulfurreducens | CoA + (2S)-2-hydroxy-2-methylbutanedioate | - |
r | |
4.1.3.22 | acetate + pyruvate | citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM | Geobacter sulfurreducens | citramalate | - |
? | |
4.1.3.22 | acetate + pyruvate | citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM | Geobacter sulfurreducens ATCC 51573 / DSM 12127 | citramalate | - |
? | |
4.1.3.22 | acetate + pyruvate | citramalate synthase appears to have a high affinity for pyruvate, with 52.5% +/- 4.9% of the activity remaining when the pyruvate concentration is reduced from 1 mM to 0.1 mM | Geobacter sulfurreducens DSM 12127 | citramalate | - |
? | |
4.1.3.22 | citramalate | - |
Geobacter sulfurreducens | 2-oxobutanoate + ? | - |
? | |
4.1.3.22 | citramalate | - |
Geobacter sulfurreducens ATCC 51573 / DSM 12127 | 2-oxobutanoate + ? | - |
? | |
4.1.3.22 | citramalate | - |
Geobacter sulfurreducens DSM 12127 | 2-oxobutanoate + ? | - |
? | |
4.3.1.17 | L-serine | - |
Geobacter sulfurreducens | pyruvate + NH3 | - |
? | |
4.3.1.17 | L-serine | - |
Geobacter sulfurreducens ATCC 51573 / DSM 12127 | pyruvate + NH3 | - |
? | |
4.3.1.17 | L-serine | - |
Geobacter sulfurreducens DSM 12127 | pyruvate + NH3 | - |
? | |
4.3.1.17 | additional information | GSU0486 mainly catalyzes the deamination of L-threonine, EC 4.3.1.19 | Geobacter sulfurreducens | ? | - |
? | |
4.3.1.17 | additional information | GSU0486 mainly catalyzes the deamination of L-threonine, EC 4.3.1.19 | Geobacter sulfurreducens ATCC 51573 / DSM 12127 | ? | - |
? | |
4.3.1.17 | additional information | GSU0486 mainly catalyzes the deamination of L-threonine, EC 4.3.1.19 | Geobacter sulfurreducens DSM 12127 | ? | - |
? | |
4.3.1.19 | L-threonine | - |
Geobacter sulfurreducens | 2-oxobutanoate + NH3 | - |
? | |
4.3.1.19 | L-threonine | a step in the pathway for the biosynthesis of isoleucine via isoleucine precursor 2-oxobutanoate generated from threonine, this pathway accounts for a minor fraction of isoleucine biosynthesis, while the majority of isoleucine is instead derived from acetyl-coenzyme A and pyruvate, possibly via the citramalate pathway, overview | Geobacter sulfurreducens | 2-oxobutanoate + NH3 | - |
? | |
4.3.1.19 | L-threonine | - |
Geobacter sulfurreducens DSM 12127 | 2-oxobutanoate + NH3 | - |
? | |
4.3.1.19 | L-threonine | a step in the pathway for the biosynthesis of isoleucine via isoleucine precursor 2-oxobutanoate generated from threonine, this pathway accounts for a minor fraction of isoleucine biosynthesis, while the majority of isoleucine is instead derived from acetyl-coenzyme A and pyruvate, possibly via the citramalate pathway, overview | Geobacter sulfurreducens DSM 12127 | 2-oxobutanoate + NH3 | - |
? | |
4.3.1.19 | additional information | GSU0486 also catalyzes the deamination of L-serine, EC 4.3.1.17 | Geobacter sulfurreducens | ? | - |
? | |
4.3.1.19 | additional information | GSU0486 also catalyzes the deamination of L-serine, EC 4.3.1.17 | Geobacter sulfurreducens DSM 12127 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.3.21 | CimA | - |
Geobacter sulfurreducens |
2.3.3.21 | citramalate lyase | - |
Geobacter sulfurreducens |
4.1.3.22 | CimA | - |
Geobacter sulfurreducens |
4.1.3.22 | citramalate synthase | - |
Geobacter sulfurreducens |
4.1.3.22 | GSU1798 | represents a novel phylogenetic variant of the enzyme | Geobacter sulfurreducens |
4.3.1.17 | GSU0486 | - |
Geobacter sulfurreducens |
4.3.1.17 | tdcB | - |
Geobacter sulfurreducens |
4.3.1.19 | GSU0486 | - |
Geobacter sulfurreducens |
4.3.1.19 | tdcB | - |
Geobacter sulfurreducens |
4.3.1.19 | Thr ammonia-lyase | - |
Geobacter sulfurreducens |
4.3.1.19 | threonine ammonia-lyase | - |
Geobacter sulfurreducens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.21 | 37 | - |
enzyme assay | Geobacter sulfurreducens |