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Literature summary extracted from

  • Belchik, S.M.; Xun, L.
    Functions of flavin reductase and quinone reductase in 2,4,6-trichlorophenol degradation by Cupriavidus necator JMP134 (2008), J. Bacteriol., 190, 1615-1619.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.6.5.2 degradation TcpB is acting as a quinone reductase for 6-chlorohydroxyquinone reduction during 2,4,6-trichlorophenol degradation, a toxic pollutant Cupriavidus pinatubonensis JMP134

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.2 into the pET30-LIC vector, overexpressed in Escherichia coli BL21(DE3) Cupriavidus pinatubonensis JMP134

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.2 0.0096
-
 FMN
-
 Cupriavidus pinatubonensis JMP134
1.6.5.2 0.0863
-
 menadione
-
 Cupriavidus pinatubonensis JMP134
1.6.5.2 0.2311
-
 NADH
-
 Cupriavidus pinatubonensis JMP134

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.2 21000
-
 2 * 21000, SDS-PAGE Cupriavidus pinatubonensis JMP134

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Cupriavidus pinatubonensis JMP134
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.2 purified as a C-terminal His tag fusion by ultracentrifugation and gel filtration, to apparent homogeneity Cupriavidus pinatubonensis JMP134

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.2 3.5
-
 with FMN as substrate Cupriavidus pinatubonensis JMP134
1.6.5.2 8.1
-
 with ferricyanide and FAD as substrates Cupriavidus pinatubonensis JMP134
1.6.5.2 35.3
-
 with menadione and FAD as substrates Cupriavidus pinatubonensis JMP134
1.6.5.2 179.1
-
 with ferricyanide and FMN as substrates Cupriavidus pinatubonensis JMP134
1.6.5.2 911.4
-
 with menadione and FMN as substrates Cupriavidus pinatubonensis JMP134

Storage Stability

EC Number Storage Stability Organism
1.6.5.2 -80°C, KPi buffer, 10% glycerol, several months Cupriavidus pinatubonensis JMP134

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 2-chloro-6-hydroxy-1,4-benzoquinone + NADH
-
 Cupriavidus pinatubonensis JMP134 6-chlorobenzene-1,2,4-triol + NAD+
-
 ?
1.6.5.2 FAD + NADH
-
 Cupriavidus pinatubonensis JMP134 ?
-
 ?
1.6.5.2 ferricyanide + FAD + NADH
-
 Cupriavidus pinatubonensis JMP134 ?
-
 ?
1.6.5.2 ferricyanide + FMN + NADH
-
 Cupriavidus pinatubonensis JMP134 ?
-
 ?
1.6.5.2 FMN + NADH
-
 Cupriavidus pinatubonensis JMP134 ?
-
 ?
1.6.5.2 menadione + FAD + NADH
-
 Cupriavidus pinatubonensis JMP134 ?
-
 ?
1.6.5.2 menadione + FMN + NADH
-
 Cupriavidus pinatubonensis JMP134 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.2 dimer 2 * 21000, SDS-PAGE Cupriavidus pinatubonensis JMP134

Synonyms

EC Number Synonyms Comment Organism
1.6.5.2 Quinone reductase
-
 Cupriavidus pinatubonensis JMP134
1.6.5.2 TcpB
-
 Cupriavidus pinatubonensis JMP134

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.5.2 350.7
-
 menadione
-
 Cupriavidus pinatubonensis JMP134

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 FAD
-
 Cupriavidus pinatubonensis JMP134
1.6.5.2 FMN
-
 Cupriavidus pinatubonensis JMP134
1.6.5.2 NADH
-
 Cupriavidus pinatubonensis JMP134