EC Number | Cloned (Comment) | Organism |
---|---|---|
6.2.1.32 | gene pqsA is part of the pqsABCDE operon, sequence comparisons, expression of the His-tagged enzyme in Escherichia coli | Pseudomonas aeruginosa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.32 | 2-amino-3-chlorobenzoate | - |
Pseudomonas aeruginosa | |
6.2.1.32 | 3-fluoro-O-anisidine | - |
Pseudomonas aeruginosa | |
6.2.1.32 | 5-nitroanthranilonitrile | - |
Pseudomonas aeruginosa | |
6.2.1.32 | anthranilonitrile | - |
Pseudomonas aeruginosa | |
6.2.1.32 | methylanthranilate | i.e. 2-aminobenzoate methyl ester | Pseudomonas aeruginosa | |
6.2.1.32 | additional information | effects of substrate analogues on in vitro anthranilate-CoA ligase activity and in vivo PQS production in Pseudomonas aeruginosa, overview | Pseudomonas aeruginosa | |
6.2.1.32 | salicylate | - |
Pseudomonas aeruginosa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.32 | Mg2+ | - |
Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.32 | ATP + anthranilate + CoA | Pseudomonas aeruginosa | the Pseudomonas quinolone signal PQS is a 3-hydroxy-4-quinolone with a 2-alkyl substitution, which is synthesized by the condensation of anthranilic acid with a 3-keto-fatty acid, pathway for PQS biosynthesis, anthranilate is activated by PqsA to form anthraniloyl-CoA, and this is condensed with 2-oxo-decanoyl-ACP which is recruited from the fatty acid biosynthetic pathway, overview | AMP + diphosphate + anthranilyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.32 | Pseudomonas aeruginosa | Q9I4X3 | gene pqsA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.32 | recombinant His-tagged PqsA 58fold from Escherichia coli by ammonium sulfate fractionation, metal affinity and hydrophobic interaction chromatography, and anion exchange chromatography | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.32 | ATP + anthranilate + CoA | - |
Pseudomonas aeruginosa | AMP + diphosphate + anthranilyl-CoA | - |
? | |
6.2.1.32 | ATP + anthranilate + CoA | the Pseudomonas quinolone signal PQS is a 3-hydroxy-4-quinolone with a 2-alkyl substitution, which is synthesized by the condensation of anthranilic acid with a 3-keto-fatty acid, pathway for PQS biosynthesis, anthranilate is activated by PqsA to form anthraniloyl-CoA, and this is condensed with 2-oxo-decanoyl-ACP which is recruited from the fatty acid biosynthetic pathway, overview | Pseudomonas aeruginosa | AMP + diphosphate + anthranilyl-CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.2.1.32 | anthranilate-coenzyme A ligase | - |
Pseudomonas aeruginosa |
6.2.1.32 | PqsA | - |
Pseudomonas aeruginosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.2.1.32 | 37 | - |
assay at | Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.32 | 8.5 | - |
- |
Pseudomonas aeruginosa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.32 | 7 | 9 | - |
Pseudomonas aeruginosa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.32 | ATP | - |
Pseudomonas aeruginosa |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.32 | 0.0129 | - |
2-amino-3-chlorobenzoate | pH 8.0, 37°C | Pseudomonas aeruginosa | |
6.2.1.32 | 0.0183 | - |
salicylate | pH 8.0, 37°C | Pseudomonas aeruginosa | |
6.2.1.32 | 0.037 | - |
5-nitroanthranilonitrile | pH 8.0, 37°C | Pseudomonas aeruginosa | |
6.2.1.32 | 0.0897 | - |
3-fluoro-O-anisidine | pH 8.0, 37°C | Pseudomonas aeruginosa | |
6.2.1.32 | 1.3 | - |
anthranilonitrile | pH 8.0, 37°C | Pseudomonas aeruginosa | |
6.2.1.32 | 5.8 | - |
methylanthranilate | pH 8.0, 37°C | Pseudomonas aeruginosa |