EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.37 | overexpression in Escherichia coli in a soluble form | Pseudomonas protegens Pf-5 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.37 | 0.0032 | - |
FAD | pH 7.0, 30°C | Pseudomonas protegens Pf-5 | |
1.5.1.37 | 0.0286 | - |
FMN | pH 7.0, 30°C | Pseudomonas protegens Pf-5 | |
1.5.1.37 | 0.0383 | - |
riboflavin | pH 7.0, 30°C | Pseudomonas protegens Pf-5 | |
1.5.1.37 | 0.0431 | - |
NADH | pH 7.0, 30°C | Pseudomonas protegens Pf-5 | |
1.5.1.37 | 0.0584 | - |
Lumiflavin | pH 7.0, 30°C | Pseudomonas protegens Pf-5 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.37 | additional information | - |
the apparent molecular mass of the PrnD-PrnF complex appears to be approximately 120000 Da (gel filtration), confirming a 1:1 stoichiometry for binding of the two proteins, PrnD (86000 Da) and PrnF (39000 Da) | Pseudomonas protegens Pf-5 |
1.5.1.37 | 19000 | - |
2 * 19000, calculation from sequence | Pseudomonas protegens Pf-5 |
1.5.1.37 | 39000 | - |
gel filtration | Pseudomonas protegens Pf-5 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.37 | FAD + NADH + H+ | Pseudomonas protegens Pf-5 | involved in pyrrolnitrin biosynthesis. PrnF is the flavin:NADH reductase component of the two-component arylamine oxygenase system in Pseudomonas fluorescens Pf-5. PrnF reduces FAD to FADH2, which is then directly transferred to PrnD, where it is used by PrnD to catalyze the oxygenation of aminopyrrolnitrin. The PrnD oxygenase component requires a direct interaction with the PrnF reductase component to oxygenate arylamine | FADH2 + NAD+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.37 | Pseudomonas protegens Pf-5 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.37 | - |
Pseudomonas protegens Pf-5 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.37 | FAD + NADH + H+ | involved in pyrrolnitrin biosynthesis. PrnF is the flavin:NADH reductase component of the two-component arylamine oxygenase system in Pseudomonas fluorescens Pf-5. PrnF reduces FAD to FADH2, which is then directly transferred to PrnD, where it is used by PrnD to catalyze the oxygenation of aminopyrrolnitrin. The PrnD oxygenase component requires a direct interaction with the PrnF reductase component to oxygenate arylamine | Pseudomonas protegens Pf-5 | FADH2 + NAD+ | - |
? | |
1.5.1.37 | FAD + NADH + H+ | no activity with NADPH. PrnF reduces FAD to FADH2, which is then directly transferred to aminopyrrolnitrin oxygenase (PrnD), where it is used by PrnD to catalyze the oxygenation of aminopyrrolnitrin. The PrnD oxygenase component requires a direct interaction with the PrnF reductase component to oxygenate arylamine. Other flavin reductases present in the host cell would not supplant the role of PrnF. The turnover rate of PrnD in the presence of PrnF is almost two times higher than that in the presence of the Escherichia coli flavin SsuE reductase (EC 1.5.1.29) | Pseudomonas protegens Pf-5 | FADH2 + NAD+ | - |
? | |
1.5.1.37 | FMN + NADH + H+ | 12% of the activity with FAD | Pseudomonas protegens Pf-5 | FMNH2 + NAD+ | - |
? | |
1.5.1.37 | lumiflavin + NADH + H+ | 6% of the activity with FAD | Pseudomonas protegens Pf-5 | reduced lumiflavin + NAD+ | - |
? | |
1.5.1.37 | additional information | methylene blue and ferricyanide are less than 5% as effective as FAD | Pseudomonas protegens Pf-5 | ? | - |
? | |
1.5.1.37 | riboflavin + NADH + H+ | 9% of the activity with FAD | Pseudomonas protegens Pf-5 | reduced riboflavin + NAD+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.37 | dimer | 2 * 19000, calculation from sequence | Pseudomonas protegens Pf-5 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.37 | flavin:NADH reductase | - |
Pseudomonas protegens Pf-5 |
1.5.1.37 | PrnF | - |
Pseudomonas protegens Pf-5 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.37 | 4 | - |
pH 7.0, 1 mM dithiothreitol, 50 mM Tris-HCl, 50% loss of activity after 12 days | Pseudomonas protegens Pf-5 |
1.5.1.37 | 16 | - |
pH 7.0, 1 mM dithiothreitol, 50 mM Tris-HCl, 50% loss of activity after 5 days | Pseudomonas protegens Pf-5 |
1.5.1.37 | 30 | - |
pH 7.0, 1 mM dithiothreitol, 50 mM Tris-HCl, 50% loss of activity after 1 day | Pseudomonas protegens Pf-5 |
1.5.1.37 | 42 | - |
pH 7.0, 1 mM dithiothreitol, 50 mM Tris-HCl, 50% loss of activity after 30 min | Pseudomonas protegens Pf-5 |
1.5.1.37 | 50 | - |
pH 7.0, 1 mM dithiothreitol, 50 mM Tris-HCl, 50% loss of activity after 1 min | Pseudomonas protegens Pf-5 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.37 | 62.6 | - |
NADH | pH 7.0, 30°C | Pseudomonas protegens Pf-5 | |
1.5.1.37 | 65 | - |
FAD | pH 7.0, 30°C | Pseudomonas protegens Pf-5 | |
1.5.1.37 | 66.5 | - |
Lumiflavin | pH 7.0, 30°C | Pseudomonas protegens Pf-5 | |
1.5.1.37 | 68.2 | - |
riboflavin | pH 7.0, 30°C | Pseudomonas protegens Pf-5 | |
1.5.1.37 | 72.4 | - |
FMN | pH 7.0, 30°C | Pseudomonas protegens Pf-5 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.37 | 7 | - |
- |
Pseudomonas protegens Pf-5 |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.37 | 6 | 8 | pH 6.0: 73% of maximal activity, pH 8.0: 81% of maximal activity | Pseudomonas protegens Pf-5 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.37 | FAD | - |
Pseudomonas protegens Pf-5 | |
1.5.1.37 | FMN | 12% of the activity with FAD | Pseudomonas protegens Pf-5 | |
1.5.1.37 | Lumiflavin | 6% of the activity with FAD | Pseudomonas protegens Pf-5 | |
1.5.1.37 | NADH | no activity with NADPH | Pseudomonas protegens Pf-5 | |
1.5.1.37 | riboflavin | 9% of the activity with FAD | Pseudomonas protegens Pf-5 |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.5.1.37 | Pseudomonas protegens Pf-5 | calculated from sequence | - |
6.3 |
1.5.1.37 | Pseudomonas protegens Pf-5 | isoelectric focusing | - |
6.5 |