Any feedback?
Literature summary extracted from
- Purification and characterization of the PcrA helicase of Bacillus anthracis (2003), J. Bacteriol., 185, 6633-6639.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.3 | single-stranded DNA | stimulates | Bacillus anthracis |  |
| 5.6.2.4 | single-stranded DNA | stimulates | Bacillus anthracis | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.6.2.3 | PcrA protein is overexpressed with a His6 fusion at its amino-terminal end | Bacillus anthracis |
| 5.6.2.4 | PcrA protein is overexpressed with a His6 fusion at its amino-terminal end | Bacillus anthracis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.6.2.3 | Bacillus anthracis | A0A0F7RI68 | - |
- |
| 5.6.2.4 | Bacillus anthracis | A0A0F7RI68 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.6.2.3 | - |
Bacillus anthracis |
| 5.6.2.4 | - |
Bacillus anthracis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.2.3 | ATP + H2O | PcrA shows 3' to 5' as well as 5' to 3' helicase activities, with substrates containing a duplex region and a 3' or 5' ss poly(dT) tail. PcrA also efficiently unwinds oligonucleotides containing a duplex region and a 5' or 3' ss tail with the potential to form a secondary structure | Bacillus anthracis | ADP + phosphate | - |
? | |
| 5.6.2.4 | ATP + H2O | PcrA shows 3' to 5' as well as 5' to 3' helicase activities, with substrates containing a duplex region and a 3' or 5' ss poly(dT) tail. PcrA also efficiently unwinds oligonucleotides containing a duplex region and a 5' or 3' ss tail with the potential to form a secondary structure | Bacillus anthracis | ADP + phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.6.2.3 | PcrA | - |
Bacillus anthracis |
| 5.6.2.3 | PcrA helicase | - |
Bacillus anthracis |
| 5.6.2.4 | PcrA | - |
Bacillus anthracis |
| 5.6.2.4 | PcrA helicase | - |
Bacillus anthracis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
